LOCUS       sequence-290515-        9170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPAN2-del486-493GSG-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9170)
  AUTHORS   Jonas S, Christie M, Peter D, Bhandari D, Loh B, Huntzinger E, 
            Weichenrieder O, Izaurralde E
  TITLE     An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to 
            mediate mRNA deadenylation and decay.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):599-608. doi: 10.1038/nsmb.2837.
            Epub 2014 Jun 1.
  PUBMED    24880343
REFERENCE   2  (bases 1 to 9170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2837";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "599-608"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmPAN2-del486-493GSG-dsRNAr...
FEATURES             Location/Qualifiers
     source          1..9170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6455..6496
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6506..6523
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6546..6563)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6596..6730
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6620..6639)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6674..6693
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7352..7940)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7432..7451)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8111..8902,8903..8971))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8734..8753
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8972..9076)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9144..9162
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catggactat gtctactgtg gaacggatcc
     2761 gattggagct agcgaggata tcctgtccgt gtacgatgcc ggcagtgccc ccggcaatgg
     2821 tcatttcagc ccgtccttta acggctttaa tattggaacg acggaccccg agtacgtcga
     2881 actggtcccg gtcctggctg atggaggtga acattttgga gtgagctccg tcgcctttga
     2941 tgattatgag gaattgttgt ggatgggaaa tcaaggagga catgtgacca gctattatac
     3001 caatagcatg caaaaatata ccagcttcca agtgcatgct acggatatcg tccgtgatat
     3061 tagcacgttg gatagcggcg tcttggccct gacccaaacg agcttgcgcc accaaattcg
     3121 tcgcggcttg ccgaaattca cgtttaagag caataatatg aaagaaatgg tctccatgtt
     3181 gcaactgtcg ccccaccgct tggtgatggc tggcttgcaa gatgaactga ttgattttga
     3241 tttgcgtacc ctgaaagaga cgcgtattga acacgtcggc gctggaggct gtaccgtctt
     3301 gcgtaaaaac tcgcgctact tgttcgctgg agatcaattg ggcaccgtta ccctgcgtga
     3361 tttgaattcc ttgtccgtgc aacataccat taaaacccat acgaatattc tgagcgattt
     3421 ttccgtgcaa ggcaacttgc tgattagttg tggctacagc ggaaggcaaa ataacctggc
     3481 catcgaccgc ttcctaatgg tctacgatct gcgcatgctg cgcctgatcg cgcccattca
     3541 agtgatgatt gatccgcaga tgttgaagtt cctgccatcg ctgacatccc aactggccgt
     3601 cgtgtccagc tacggccagg ttcagctggt ggacactgtg gagctcagcg agccgcgagt
     3661 atccatgtac cagatcaaca ccaacggcag ccagtgcctc agcttcgaca ttagctcctc
     3721 ttcacaggcc atggcatttg gggaccagtc gggccacatc aacatgattg ccgctgtaca
     3781 aacgcctcag ccgcagttca atctctactc gcgaagcaca gagtttgcgg acgtggtgcc
     3841 acagctgccg atggtctcca ttacggacac caactttccg ctatcgtcgg tcatgctacc
     3901 gcacttgacg acgggcacac aatggttctc agattggccg gaggaactgc tgcgctaccg
     3961 ctaccacagg cccaagacca ttgatcccga agtgctgagc aacatgaaaa tgcagggacc
     4021 gattggatac tcgccaaacc cacgcaccgc ccgtcgtaat caaattccgt acgtgattga
     4081 gcagggcggc gtgtgcagtc cgaacggaaa tggaactgcg gcggcgacca aggcggagaa
     4141 cggtgtgaaa atcattccac ggcggtatcg caaagtcgag ctgaagggca gcggctttga
     4201 ctttgaccag cataatcaaa catgtttcgc ggggctggag gcaacgctac cgaactccta
     4261 ctgcaatgct atgctacaga ttttgtattt caccgacgcg cttcgcgtga agctgctgga
     4321 gcattcgtgc atcaaggagt tttgcctgtc ctgcgaacta gggttcctct tcaacatgct
     4381 ggataaaagt accgcttctt caccttgcca ggccagcaat ttcctgcgat ctttccgcac
     4441 tgtgccagag gcctcggcac tgggattgat tctcaccgac cgctcatcga acgtcaatct
     4501 aatatcgctg atacagaact ggaaccgatt cattttgcat caaatgcatt acgaaatatt
     4561 cgattctagc aagaacgcat ccacctacag cggatctgtg caaacatcaa cgaacgcgga
     4621 aaacgcgggc tcttcagaaa caagtggctc ctcggacctt tacgattcca taagtgatga
     4681 gaactccaag gaggacgatc gggaacgcag taagatcaac gcagaaactg acatcagtaa
     4741 gatctttggg accaagcaaa tctgtataaa ccgctgcatc aaatgccaag aagagaagat
     4801 aaaggaaagc attctacttg cctgcaattt gtcgtatccc aaccacatca aagatagtga
     4861 tcagtatttc aactttggca ccattttgaa gcgatctctc agctccgaga agagtattca
     4921 ggccttctgc gagagatgta agaagttttc gccaaccaat cagagcgtga aggtgacttc
     4981 gcttccgcaa attctgtcca ttaactgtgg cctcaacaac gaaaaagaca ttaccttcct
     5041 gaagcgccag ctcaatcgct gcagcgaaaa gacgactgtt gatgcggccg cttccttgag
     5101 cacaagcaag ccgtgtcgat atggagccaa ctgttcgcga agtgactgcc atttcatgca
     5161 tccggatcga aaatctccat cgcacacaag ccaaccgaat gctgtcaaca attctcccaa
     5221 tggacgccaa aagtcctggt ttccccttac atttactatg ggcatcaatg accaaggtga
     5281 ggtgcaggtg caaactcaat cagatgcaag cagtggcaaa tcggaacagg aggaggaaac
     5341 ggaaaagcca ccaacaaagg gcttggacaa caatcggatg tacgcgctgc atgccgtggt
     5401 gtgccaagtc gatgatggta cccaaaagaa cttggtatcg ttgataaatg tgcagcggcc
     5461 ataccacacc atgaaactgg ctgaatctgc agatgaccct cagagtcaat ggtatatttt
     5521 taatgatttt agcatatcgc ccgtttcgcc acaagaatcg gtatggttca cattggattg
     5581 gaaagttcct tgcatactct tctacagaca cgtagaggat gactctgagt ctgctagtac
     5641 tactagctcg accgtcacgg aaagtgaaga aaccattcca tcagagagca gcagcggctc
     5701 gccaacaaat ctatccaacc cttttctaga ggaaattgtg agtcctatgc taggcaactt
     5761 aagtgcagac gccacgctgc agcccctgca gtcagatgaa atgccgcagt cgggtgatct
     5821 ggtcgcaatg gatgccgagt tcgttaccct aaatccagag gaaaatgaga ttcgcccgga
     5881 tggcaaaacg gcgaccatta agccatgcca catgagtgtg gcccggattt catgtatacg
     5941 aggacaaggc ccggctgaag gagttccatt catggacgat tacatatcca cccaggagaa
     6001 ggtcgtggac tacctaactc agttctcggg aatcaagcct ggcgacttgg acgctaactt
     6061 cagcaaaaag cgactgactg ccttgaagta ctcataccag aaattaaaat acctcgttga
     6121 cgtaggagta atatttgtcg gacatggtct gaagaacgac tttagggtca taaacatcta
     6181 tgtaccgtcg gagcagataa tagatacggt gcacctgttt cacatgccac atcatcgcat
     6241 ggtttcccta agatttctag cctggcattt cttgggtact aagattcagt ccgagacgca
     6301 cgactccatc gaagatgcac ggaccactct gcagctctac aagcactatt tgaagctgca
     6361 ggaggagaag aagtttgcca acgctctcaa gaatctttac gaacgcggaa agcagctgca
     6421 gtggaaggtt ccagaggact gaccgcggtt cgaaggtaag cctatcccta accctctcct
     6481 cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacccgctg
     6541 atcagcctcg actgtgcctt ctaaggcctg agctcgctga tcagcctcga tcgaggatcc
     6601 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     6661 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     6721 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     6781 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcagtc
     6841 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     6901 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     6961 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     7021 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     7081 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
     7141 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     7201 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     7261 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccag caaaaggcca
     7321 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7381 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7441 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7501 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     7561 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7621 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7681 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7741 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     7801 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7861 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7921 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7981 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8041 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8101 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8161 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8221 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8281 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8341 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8401 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
     8461 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8521 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8581 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8641 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8701 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
     8761 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8821 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8881 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8941 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9001 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9061 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     9121 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
//
