LOCUS       sequence-290526-        9185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPAN2-T97RH102G-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Jonas S, Christie M, Peter D, Bhandari D, Loh B, Huntzinger E, 
            Weichenrieder O, Izaurralde E
  TITLE     An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to 
            mediate mRNA deadenylation and decay.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):599-608. doi: 10.1038/nsmb.2837.
            Epub 2014 Jun 1.
  PUBMED    24880343
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2837";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "599-608"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmPAN2-T97RH102G-dsRNAres_Y
FEATURES             Location/Qualifiers
     source          1..9185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6470..6511
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6521..6538
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6561..6578)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6611..6745
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6635..6654)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6689..6708
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7367..7955)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7447..7466)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8126..8917,8918..8986))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8749..8768
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8987..9091)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9159..9177
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catggactat gtctactgtg gaacggatcc
     2761 gattggagct agcgaggata tcctgtccgt gtacgatgcc ggcagtgccc ccggcaatgg
     2821 tcatttcagc ccgtccttta acggctttaa tattggaacg acggaccccg agtacgtcga
     2881 actggtcccg gtcctggctg atggaggtga acattttgga gtgagctccg tcgcctttga
     2941 tgattatgag gaattgttgt ggatgggaaa tcaaggagga catgtgacca gctattatac
     3001 caatagcatg caaaaatatc gcagcttcca agtgggtgct acggatatcg tccgtgatat
     3061 tagcacgttg gatagcggcg tcttggccct gacccaaacg agcttgcgcc accaaattcg
     3121 tcgcggcttg ccgaaattca cgtttaagag caataatatg aaagaaatgg tctccatgtt
     3181 gcaactgtcg ccccaccgct tggtgatggc tggcttgcaa gatgaactga ttgattttga
     3241 tttgcgtacc ctgaaagaga cgcgtattga acacgtcggc gctggaggct gtaccgtctt
     3301 gcgtaaaaac tcgcgctact tgttcgctgg agatcaattg ggcaccgtta ccctgcgtga
     3361 tttgaattcc ttgtccgtgc aacataccat taaaacccat acgaatattc tgagcgattt
     3421 ttccgtgcaa ggcaacttgc tgattagttg tggctacagc ggaaggcaaa ataacctggc
     3481 catcgaccgc ttcctaatgg tctacgatct gcgcatgctg cgcctgatcg cgcccattca
     3541 agtgatgatt gatccgcaga tgttgaagtt cctgccatcg ctgacatccc aactggccgt
     3601 cgtgtccagc tacggccagg ttcagctggt ggacactgtg gagctcagcg agccgcgagt
     3661 atccatgtac cagatcaaca ccaacggcag ccagtgcctc agcttcgaca ttagctcctc
     3721 ttcacaggcc atggcatttg gggaccagtc gggccacatc aacatgattg ccgctgtaca
     3781 aacgcctcag ccgcagttca atctctactc gcgaagcaca gagtttgcgg acgtggtgcc
     3841 acagctgccg atggtctcca ttacggacac caactttccg ctatcgtcgg tcatgctacc
     3901 gcacttgacg acgggcacac aatggttctc agattggccg gaggaactgc tgcgctaccg
     3961 ctaccacagg cccaagacca ttgatcccga agtgctgagc aacatgaaaa tgcagggacc
     4021 gattggatac tcgccaaacc cacgcaccgc ccgtcgtaat caaattccgt acgtgattga
     4081 gcagggcggc gtgtgcagtc cgaacggaaa tggaactgcg gcggcgacca aggcggagaa
     4141 cggtgtgaaa atcattccac ggcggtatcg caaagtcgag ctgaagtaca cgaagctggg
     4201 cacccaggac tttgactttg accagcataa tcaaacatgt ttcgcggggc tggaggcaac
     4261 gctaccgaac tcctactgca atgctatgct acagattttg tatttcaccg acgcgcttcg
     4321 cgtgaagctg ctggagcatt cgtgcatcaa ggagttttgc ctgtcctgcg aactagggtt
     4381 cctcttcaac atgctggata aaagtaccgc ttcttcacct tgccaggcca gcaatttcct
     4441 gcgatctttc cgcactgtgc cagaggcctc ggcactggga ttgattctca ccgaccgctc
     4501 atcgaacgtc aatctaatat cgctgataca gaactggaac cgattcattt tgcatcaaat
     4561 gcattacgaa atattcgatt ctagcaagaa cgcatccacc tacagcggat ctgtgcaaac
     4621 atcaacgaac gcggaaaacg cgggctcttc agaaacaagt ggctcctcgg acctttacga
     4681 ttccataagt gatgagaact ccaaggagga cgatcgggaa cgcagtaaga tcaacgcaga
     4741 aactgacatc agtaagatct ttgggaccaa gcaaatctgt ataaaccgct gcatcaaatg
     4801 ccaagaagag aagataaagg aaagcattct acttgcctgc aatttgtcgt atcccaacca
     4861 catcaaagat agtgatcagt atttcaactt tggcaccatt ttgaagcgat ctctcagctc
     4921 cgagaagagt attcaggcct tctgcgagag atgtaagaag ttttcgccaa ccaatcagag
     4981 cgtgaaggtg acttcgcttc cgcaaattct gtccattaac tgtggcctca acaacgaaaa
     5041 agacattacc ttcctgaagc gccagctcaa tcgctgcagc gaaaagacga ctgttgatgc
     5101 ggccgcttcc ttgagcacaa gcaagccgtg tcgatatgga gccaactgtt cgcgaagtga
     5161 ctgccatttc atgcatccgg atcgaaaatc tccatcgcac acaagccaac cgaatgctgt
     5221 caacaattct cccaatggac gccaaaagtc ctggtttccc cttacattta ctatgggcat
     5281 caatgaccaa ggtgaggtgc aggtgcaaac tcaatcagat gcaagcagtg gcaaatcgga
     5341 acaggaggag gaaacggaaa agccaccaac aaagggcttg gacaacaatc ggatgtacgc
     5401 gctgcatgcc gtggtgtgcc aagtcgatga tggtacccaa aagaacttgg tatcgttgat
     5461 aaatgtgcag cggccatacc acaccatgaa actggctgaa tctgcagatg accctcagag
     5521 tcaatggtat atttttaatg attttagcat atcgcccgtt tcgccacaag aatcggtatg
     5581 gttcacattg gattggaaag ttccttgcat actcttctac agacacgtag aggatgactc
     5641 tgagtctgct agtactacta gctcgaccgt cacggaaagt gaagaaacca ttccatcaga
     5701 gagcagcagc ggctcgccaa caaatctatc caaccctttt ctagaggaaa ttgtgagtcc
     5761 tatgctaggc aacttaagtg cagacgccac gctgcagccc ctgcagtcag atgaaatgcc
     5821 gcagtcgggt gatctggtcg caatggatgc cgagttcgtt accctaaatc cagaggaaaa
     5881 tgagattcgc ccggatggca aaacggcgac cattaagcca tgccacatga gtgtggcccg
     5941 gatttcatgt atacgaggac aaggcccggc tgaaggagtt ccattcatgg acgattacat
     6001 atccacccag gagaaggtcg tggactacct aactcagttc tcgggaatca agcctggcga
     6061 cttggacgct aacttcagca aaaagcgact gactgccttg aagtactcat accagaaatt
     6121 aaaatacctc gttgacgtag gagtaatatt tgtcggacat ggtctgaaga acgactttag
     6181 ggtcataaac atctatgtac cgtcggagca gataatagat acggtgcacc tgtttcacat
     6241 gccacatcat cgcatggttt ccctaagatt tctagcctgg catttcttgg gtactaagat
     6301 tcagtccgag acgcacgact ccatcgaaga tgcacggacc actctgcagc tctacaagca
     6361 ctatttgaag ctgcaggagg agaagaagtt tgccaacgct ctcaagaatc tttacgaacg
     6421 cggaaagcag ctgcagtgga aggttccaga ggactgaccg cggttcgaag gtaagcctat
     6481 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg
     6541 agtttaaacc cgctgatcag cctcgactgt gccttctaag gcctgagctc gctgatcagc
     6601 ctcgatcgag gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     6661 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     6721 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     6781 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     6841 tgattatgat cagtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     6901 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     6961 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg
     7021 cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct
     7081 tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg
     7141 cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc
     7201 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
     7261 ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca
     7321 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7381 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7441 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7501 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7561 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7621 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7681 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7741 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7801 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7861 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7921 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7981 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8041 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8101 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8161 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8221 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8281 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8341 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8401 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
     8461 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8521 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8581 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8641 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8701 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     8761 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8821 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8881 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8941 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9001 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9061 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9121 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     9181 ttcgt
//
