LOCUS       sequence-290531-        7846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPAN3-FLLYHRtoEEEAAA-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Jonas S, Christie M, Peter D, Bhandari D, Loh B, Huntzinger E, 
            Weichenrieder O, Izaurralde E
  TITLE     An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to 
            mediate mRNA deadenylation and decay.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):599-608. doi: 10.1038/nsmb.2837.
            Epub 2014 Jun 1.
  PUBMED    24880343
REFERENCE   2  (bases 1 to 7846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2837";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "599-608"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmPAN3-FLLYHRtoEEEAAA-dsRNA...
FEATURES             Location/Qualifiers
     source          1..7846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      2768..2777
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5131..5172
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5182..5199
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5222..5239)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5272..5406
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5296..5315)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5350..5369
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6028..6616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6108..6127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6787..7578,7579..7647))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7410..7429
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7648..7752)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7820..7838
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctatgtgttc cacatacggc ctgctttcat tcgaattctc
     2761 ggacaccgcc gccatggacc cgatcttttt ttccccgacc aacggcattc ccagtgaatc
     2821 gaagctggct acatacatga accgccaaaa cgtggctacg ccctccggat acggtttgaa
     2881 caatggattt agtcttctca acctggactc cccgctcaac aagaaaagcc aagtgacccc
     2941 ccaatccccg gaatttatcc ccacccgtct cggatccacc ccgaattttt acgccccgta
     3001 ccataatgcc ccgatgcagc tatcgaacgg actgaacgga gtgaacggtc taacggctgc
     3061 tgctgctgcg gccgccgccg ctgctgctgc tgctgctgct gccgtcccga gcagcaccag
     3121 ttccgccagc agcgcttccg tcacgatttc caagaccgct aatggcggag ctagcatgct
     3181 ggctttgcag aaaacggcta gcattgcttc agttacgatt gcccagcaac aaccgcaaca
     3241 cccgcaaaag caacagcaac accccccctc cgtcggaggc ggcgccgtca gcgctgctag
     3301 cgctccgatc gctatctcgg gagccccgcc caacccggct gcggccccct ttgtgtcctc
     3361 catgagcgcc caaaccccct tgaaaggacg aggagctatg atgcgtcaag agtcgccgac
     3421 cgctgctatg atctccggag ccggtccggg cgaaaaatcg ccaccgcacg ggatgacgcc
     3481 gcacggagca tcgcccatac catcagcgct gcccaccagc gtgcaccagg agaacgtagg
     3541 cggcaccata tacttctatc cgactgccaa cgcacagaac agccagcccg tcgtcaattc
     3601 catggtggtg gatggaacgc atccggctct ccatggcgtg agtgctgtgg cgccgatgag
     3661 tgcgggcgtt ccggcggcca tgatgtacac ggggcatgtg tatccgggac cgtcctccaa
     3721 cgtggtcacc atgcagccaa agacgctgct ggagtcggcc ttctttatgc ccgacgagat
     3781 gcgcgccgaa gtcctcgccc gcaacgagat ctctaacctg atcatggacg cagcggaggc
     3841 tgcgcagcac gctctaccgc tggaggtgga gaactaccat gccctgtatc cactggaacc
     3901 gccggcgcag ccgttgcacg ccaagctcac gttccccgcc accacttata gggccacgca
     3961 caacacgacg ggctacaagt actgcctgcg aagaatacat ggtttccgcc tgcagtcgac
     4021 caagtgcatg acgctggtgg agatgtggaa gaagctgcag cacacgaatg tggtacaact
     4081 gcgtgaggtg ttcacgacga aagcatttgg tgataactct ttagtattag tgtacgacta
     4141 tcatcccggc tcacaaacat tactggccaa atacttcact ccggcgccag agaccaacgg
     4201 gtacactgat ccattccaag gcgaggcccg ccccttcagt cataagagta acatgcagcg
     4261 aacgagcaat ggaccgctgc taccggaggc caccatctgg tcgatcatca tgcagctgac
     4321 cgccggctta aaagccatcc accatgccgg tcttgcctgc aaggttctgg atcccacaaa
     4381 gattatagtg acgggcaagc gtgtacggtt tagctcatgc tgcatttcgg atataacgca
     4441 attcgatccg aatgcatcca atcccttggc gctggttaat atgcatcagc aggacgatct
     4501 gaccgctttg ggtcgcttag tattagcttt ggcctgccgc tgtcttcaat ccgtgcaacg
     4561 cgacaatgtc cagtccagca tcgatatggt cacgcgaaac tactccaccg atctgcgaaa
     4621 ctttattgtt tacctcttca ccaccaataa tcgtcgctcc gtgaccgacc tgatgccgat
     4681 gattggtgcc cgcgaatata cgcaagaaga tgcagaacag agcaaaattg atatgcagga
     4741 ggacgagttg gccaaggaga tggagaacgg gcggttatat cgcattttgg tcaaacttaa
     4801 tagcatcaac gagcgacccg actttaatct ggactgcact tggtctgaga ctggtgatag
     4861 atacatgttg aaattattcc gtgatgcttt gttccattcc gtcactgagg atggccggcc
     4921 ctggctagat cacgcagcca ttgtacaatg cctcaacaag ctggatgctg gctccataga
     4981 ggcggtgcaa ctgatgtccc gcgacgagca atcggtactc atcgtctcct atgctgaact
     5041 caagaattgt ctggagaacg ccttctccga actgatgtca agtgcggcca actgagcggc
     5101 cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt
     5161 ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca
     5221 gcctcgactg tgccttctaa ggcctgagct cgctgatcag cctcgatcga ggatccagac
     5281 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     5341 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     5401 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     5461 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcagtcgacc
     5521 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     5581 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     5641 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
     5701 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
     5761 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
     5821 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     5881 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     5941 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agccagcaaa aggccaggaa
     6001 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6061 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
     6241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     6481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     6781 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6841 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6901 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6961 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7021 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7081 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7141 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7201 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7261 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7321 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7381 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
     7441 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7501 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7561 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7621 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7681 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7741 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     7801 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgt
//
