LOCUS       sequence-29054-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Rice JJ, Daugherty PS
  TITLE     Directed evolution of a biterminal bacterial display scaffold 
            enhances the display of diverse peptides.
  JOURNAL   Protein Eng Des Sel. 2008 Jul . 21(7):435-42.
  PUBMED    18480093
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein Eng
            Des Sel. 2008 Jul . 21(7):435-42."
COMMENT     Sequence Label: pB33eCPX
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(650..934)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(708..727)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 cgcaacagcc aagcttggcc accttggcct tattagcttg cagtacggct tttctcggtg
       61 taagtgaaag aaccgatcac acccagcggg ctgttgtctt cttcatagcg gtatttcagg
      121 ttgaaaccgc ccattttgtt catttggccc tgagcgtcgc tctgtgcgta accgccagtt
      181 acagtagaag tcgcgcgacg cgatttactc ccgaagcggt aaccaacaga caaaatccag
      241 gtgcctacgt caacgctacg aatacggctc tgctcgtaag agaagtccag agcaacgttt
      301 tccatcgggt tgaactgcag acccgcaccg taggagaaac cgtagtcgct ggtgtcgtgt
      361 ttgtaggtcg ggtattcagt ggtctggaat ttaccataac ccacacccac tacaccgtag
      421 atgcttgccc agtcgttaat gcggtaagcc ggaccagcag tgatgccgta gtactggttt
      481 ttgttgtagt caccagactg cccagactgc cctccctggc cagactggcc agctacggaa
      541 gtacctgcgg tgaaagccag aactgcggcc agtgctgaaa gacatgcaat ttttttcata
      601 accacctcaa aggtaccgag ctcgaattcg ctagcccaaa aaaacgggta tggagaaaca
      661 gtagagagtt gcgataaaaa gcgtcaggta ggatccgcta atcttatgga taaaaatgct
      721 atggcatagc aaagtgtgac gccgtgcaaa taatcaatgt ggacttttct gccgtgatta
      781 tagacacttt tgttacgcgt ttttgtcatg gctttggtcc cgctttgtta cagaatgctt
      841 ttaataagcg gggttaccgg tttggttagc gagaagagcc agtaaaagac gcagtgacgg
      901 caatgtctga tgcaatatgg acaattggtt tcttctctga tgc
//
