LOCUS       sequence-290653-        9234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmUNR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9234)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 9234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmUNR_AA
FEATURES             Location/Qualifiers
     source          1..9234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6519..6560
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6570..6587
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6610..6627)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6660..6794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6684..6703)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6738..6757
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7416..8004)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7496..7515)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8175..8966,8967..9035))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8798..8817
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9036..9140)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9208..9226
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgatgaata cccaatcgaa ggtgtaccgt accggcgaag aaatctatga
     3361 caacttgcca tgcgatagtt atttcaacat gaatgccatt cgcaacctgg gaatcccaac
     3421 aacgtttccg accattggaa catttacact tgactctact acgttgggac tgcagccgca
     3481 gggtcagggc ccgtcgcccc agcagcagca gcatcaacag caacaacaac agcagcagca
     3541 gcaacatcag cagaatatgc accaccatca acatcagcag caacacatgc agcagcagca
     3601 gcaacaacaa caacatcagc agcaacacca acagcagcaa catcaacaac aacagcaaca
     3661 tcagcagcag cagcagcagc aacaacagca tccaaccatt ggaatgttcg atgccaacga
     3721 ggttaacgat gtcatccaga atcctcctca aatcggggta ttccagtcta acagcgttct
     3781 gaccaacggt gccggcagtg gctcctcgat ctttggctcc caatcgagca attcatcggc
     3841 ggcagctgca gatcccagcc agaccacccg ggaaactggc atcattgaaa agttgttgca
     3901 ttcgtatgga ttcattcagt gctgtgagcg gcaggcgcgt cttttctttc atttctcgca
     3961 attcagcggt aatattgatc atttgaaaat cggagatccc gtggagtttg agatgaccta
     4021 tgatcgccgc accggcaagc cgatagctag tcaagtatca aaaatagccc cagaggttgt
     4081 tctctccgag gagcgggtca ccggcacggt gaccaccgag ctgcgcaccg atagcgccaa
     4141 caatgtgctt aactccagcg agaccacagg tcgcataagc tatgagaacc gaggcgaatg
     4201 cttcttttta ccctatacca aagacgatgt ggaaggtaat gtcaacttgc gcgctggcga
     4261 caaggtcagc tttcaaatcg caacgaacca aagaggtaat ctaggcgcct gccatattcg
     4321 cctggaaaat ccggcccagc cggttaagta tcgtggggtt gtctgctcga tgaaggagtc
     4381 gtttggcttt atcgaacgtg ccgatgtggt caaggagatt ttcttccact tctcagaggc
     4441 tgagggcaat gttgagctgc gtcccggtga cgatgtcgag ttcaccatcc agacgcggag
     4501 cgtaagtggc agtggcgatc cgaccaatcc caatatgctc caatcaaaaa tgcagtctgc
     4561 atctgtgcct ccgcagggcc gtgaatttgc gtgcaacatc acgcgcctag ctccgggatc
     4621 agtgatcttt gaggacgtcg atagcacggt gtataagggc caggtgctta agtcgcttga
     4681 tcgcaacaac ccggtgcgcc agaacaatga tcccttgccg ggtcgtattc gttatagggc
     4741 cttggattac tccgaagtgg aggttccttt cggggacaag gatcaaaagg gtgacttcac
     4801 cctgcgccac ggcgattggg tgcagttcct gcttgccacc gatcggcgtg atcaattgca
     4861 gagggccacc tccattgccc tgttggacga gactttcaag gtttccggcg agaagcggga
     4921 gcagggcacc attgcctctc tcaaggaggg cttcggattc ctgcgctgcg tagagcgtca
     4981 ggctcgttta tttttccact ttactgaagt tttagatacg agccgcgaaa tcgatatcaa
     5041 tgatgaggta gaattcactg tcattcagga gcctggattg gcctataata actcgcgttt
     5101 gcaggccatt cgcattaaac acttgccgcc caactcagtg caatttgaaa ctctagtggc
     5161 cagcaacata gaaggatgtg taactcgcga ggcgcctaag agcccaatta aatctcaaga
     5221 tcgcgtcgag ggcggtgtga ttacctatga acatgccgat gttaaaaaga ctataatgta
     5281 ttttcttaaa gactgcgaaa aaccaccaag gattggggag cgcgtacgct tcgatattta
     5341 catggtcaaa cgtaacaagg agtgtatagc cgtcaacgta cagcaagtat ccctgcatca
     5401 gcagcaacaa cagcagcaac agcagttgca tcttaatcag tcgaatgctg gggcaaatat
     5461 caaccaaaat gaccagctgg gaggattgtc gaatggaatc agtagcagca gctcgaatgc
     5521 ttcgctacag aatggctatg tcatgcacgg cagccctggc ggcagcacca gcagcgtggg
     5581 cagcaacaat ccggttcatc tggatgaatt caagatggag aacaacaacc atgccggatc
     5641 cgatgccggt caggtgtacc gtggcttcat agccgttatg aaggagaact ttggattcat
     5701 tgagactttg tcccacgatg aggaggtctt ctttcacttt agcaactata tgggcaatcc
     5761 caattggttg gagctgggtc aggaggtgga gtacactctt gctcgtaatg ggaatacatc
     5821 cgtttctgga aactgtctgc ctgcggaaaa tgtccgtatg ctgccaaaga actctattcc
     5881 ccagccggct gtgctggaaa ccacacacaa tggcgtggta gcacgtccac tgcgttgcat
     5941 caatcccgac cagcaggaat atgccggtct cattgaaatt ctcgatgagc tacgtaccac
     6001 tgtcatttcg caacacgaat tcgggattac tagtctggtg aacaagaggg atctgctcca
     6061 gaagggcgat ctcgttagct tccgcatcga tgagagtggt cgagcagcat gcgtgaatgc
     6121 cgtaaggcag aagaagcgtg ccactgtgga ctccatcaag ggtcagtttg gttttcttaa
     6181 ctttgaggtg gaggacggca aaaaactgtt cttccacatg tctgaggtgc agggcaatac
     6241 tgtcgcttta catccaggcg ataccgtgga gttctccgtg gtcaccaatc agcgcaatgg
     6301 aaagtcgtcg gcttgcaatg ttctgaaaat aaacgatcgt ccggatcgtc tgatctcgcg
     6361 ccttaagctc aacggcgatg acactgtgcc gcgactgatc ctcattcgcg cacccaaggg
     6421 accgcagggc aagggctttt ccgttctagc tcgtcatcca cgcattccag gcaacttggt
     6481 ggagtagccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
     6541 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc
     6601 gctgatcagc ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg
     6661 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     6721 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     6781 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     6841 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     6901 agtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     6961 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     7021 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     7081 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     7141 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     7201 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     7261 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     7321 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag ccagcaaaag
     7381 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7441 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7501 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7561 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
     7621 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7681 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7741 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7801 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     7861 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7921 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7981 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8041 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8101 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8161 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8221 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8281 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8341 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8401 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8461 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     8521 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8581 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8641 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8701 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8761 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     8821 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8881 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8941 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9001 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9061 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9121 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     9181 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgt
//
