LOCUS       sequence-29072-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Singla V, Hunkapiller J, Santos N, Seol AD, Norman AR, Wakenight P, 
            Skarnes WC, Reiter JF
  TITLE     Floxin, a resource for genetically engineering mouse ESCs.
  JOURNAL   Nat Methods. 2010 Jan . 7(1):50-2.
  PUBMED    19966808
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Jan . 7(1):50-2."
COMMENT     Sequence Label: pFloxin-MCS2-IRES-MCS
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(43..90)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    108..242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(132..151)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     186..205
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cacaggaaac agctatgaac atgattacga attcgcgtcg acgaagttcc tatactttct
       61 agagaatagg aacttcggaa taggaacttc tcgacgcggt acccccggat ctagacatga
      121 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      181 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      241 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
      301 tttaaagcaa gtaaaacctc tacagatgtg atatggctga ttatgatcat tacttatcta
      361 gccccccctc gaggtcgctc tagagcgacc ccactgggct gtaggtggtg gatccgctag
      421 cgatatcact agtggcgcgc cgcatgcgat cctattatca tcgtgttttt caaaggaaaa
      481 ccacgtcccc gtggttcggg gggcctagac gtttttttaa cctcgactaa acacatgtaa
      541 agcatgtgca ccgaggcccc agatcagatc ccatacaatg gggtaccttc tgggcatcct
      601 tcagcccctt gttgaatacg cttaggagag ccatttgact ctttccacaa ctatccaact
      661 cacaacgtgg cactggggtt gtgccgcctt tgcaggtgta tcttatacac gtggcttttg
      721 gccgcagagg cacctgtcgc caggtggggg gttccgctgc ctgcaaaggg tcgctaca
//
