LOCUS       sequence-29076-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Schaffer U, Schlosser A, Muller KM, Schafer A, Katava N, Baumeister 
            R, Schulze E
  TITLE     SnAvi--a new tandem tag for high-affinity protein-complex 
            purification.
  JOURNAL   Nucleic Acids Res. 2010 Apr;38(6):e91. doi: 10.1093/nar/gkp1178. 
            Epub 2010 Jan 4.
  PUBMED    20047968
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2010-04"; volume: "38(6):e91. doi"; pages: " 
            10.1093/nar/gkp1178. Epub 2010 Jan 4"
COMMENT     Sequence Label: pBY2887
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..126
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             150..173
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             180..194
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             303..323
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             330..350
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(414..435)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(675..694)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcccggagtt atccgggacc tttaattcaa cccaacacaa tatattatag ttaaataaga
       61 attattatca aatcatttgt atattaatta aaatactata ctgtaaatta cattttattt
      121 acaatcaaag gagatatacc atggcaagct ggagccaccc gcagttcgaa aagggtgcag
      181 atgacgacga caaggtaccg cgggcccggg atccaccggt tactaccatg gctccacgac
      241 catccaacaa acgtctccag cagtccggta tgccacgacc ctccaacaag cgtcttcaac
      301 aggagaacct ttactttcaa ggtcaattgg aaaatctcta tttccaggga ccaccagcgc
      361 caccacagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      421 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      481 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      541 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
      601 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      661 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag t
//
