LOCUS       sequence-29077-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Schaffer U, Schlosser A, Muller KM, Schafer A, Katava N, Baumeister 
            R, Schulze E
  TITLE     SnAvi--a new tandem tag for high-affinity protein-complex 
            purification.
  JOURNAL   Nucleic Acids Res. 2010 Apr;38(6):e91. doi: 10.1093/nar/gkp1178. 
            Epub 2010 Jan 4.
  PUBMED    20047968
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2010-04"; volume: "38(6):e91. doi"; pages: " 
            10.1093/nar/gkp1178. Epub 2010 Jan 4"
COMMENT     Sequence Label: pBY2807
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             72..116
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             159..188
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(278..297)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    343..398
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(397..416)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      486..533
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cggagagctg tcaggattat gatattccaa ctactgcaag cgagaatttg gcagcacagg
       61 gtgagggtgg tggcctgaac gacatcttcg aggcccagaa gatcgagtgg cacgagtaag
      121 cggccgcagg agctccgggc ttctcctcaa tttccgctca tcaccaccat catcaccatc
      181 accaccacta agtgattaac ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
      241 ggccaatgcc ctggctcaca aataccactg agatcgatct ttttccctct gccaaaaatt
      301 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
      361 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
      421 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
      481 tgtaactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      541 catgcggagg aaattctcct tgaagtttcc ctggtgttca aagtaaagga gtttgcacca
      601 gacgcacctc tgttcactgg tccggcgtat taaaacacga tacattgtta ttagtacatt
      661 tattaagcgc tagattctgt gcgttgttga tttacagaca attgttgtac gtattttaat
      721 aattcattaa atttataatc tttagggtgg tatgttagag cgaaaatcaa atgattttca
      781 gcgtctttat atctg
//
