LOCUS       sequence-29081-e         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Schaffer U, Schlosser A, Muller KM, Schafer A, Katava N, Baumeister 
            R, Schulze E
  TITLE     SnAvi--a new tandem tag for high-affinity protein-complex 
            purification.
  JOURNAL   Nucleic Acids Res. 2010 Apr;38(6):e91. doi: 10.1093/nar/gkp1178. 
            Epub 2010 Jan 4.
  PUBMED    20047968
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2010-04"; volume: "38(6):e91. doi"; pages: " 
            10.1093/nar/gkp1178. Epub 2010 Jan 4"
COMMENT     Sequence Label: pBY2807
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     211..233
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     295..314
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     356..375
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        356..374
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    375..399
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        415..524
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             548..571
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             578..592
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     protein_bind    625..749
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 agcagacgtc gtttagtgac cgtcagatca ctagatgctt tattgcggta gtttatcaca
       61 gttaaattgc taacgccagt ctcgaactta acgtgcagaa gttggtcgtg aggcactggg
      121 caggtaagta tcgggccctt tgtgcggggg gagcggctcg gggctgtccg cggggggacg
      181 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
      241 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
      301 tgctggttat tgtgctgtct catcattttg gcaaagaatt ggatcggacc gaaattaata
      361 cgactcacta taggggaatt gtgagcggat aacaattccc cggagttaat ccgggacctt
      421 taattcaacc caacacaata tattatagtt aaataagaat tattatcaaa tcatttgtat
      481 attaattaaa atactatact gtaaattaca ttttatttac aatcaaagga gatataccat
      541 ggcaagctgg agccacccgc agttcgaaaa gggtgcagat gacgacgaca aggtaccgcg
      601 ggcccgggat ccaccggtat gacaacaagt ttgtacaaaa aagctgaacg agaaacgtaa
      661 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
      721 tgtaaaacac aacatatcca gtcactatgg cggccgcatt a
//
