LOCUS       sequence-290815-        5081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression of CeeIF4E3_1-215.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Peter D, Weber R, Kone C, Chung MY, Ebertsch L, Truffault V, 
            Weichenrieder O, Igreja C, Izaurralde E
  TITLE     Mextli proteins use both canonical bipartite and novel tripartite 
            binding modes to form eIF4E complexes that display differential 
            sensitivity to 4E-BP regulation.
  JOURNAL   Genes Dev. 2015 Sep 1;29(17):1835-49. doi: 10.1101/gad.269068.115. 
            Epub 2015 Aug 20.
  PUBMED    26294658
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.269068.115"; journalName: "Genes Dev"; date: 
            "2015-09-1- 1"; volume: "29"; issue: "17"; pages: "1835-49"
COMMENT     Sequence Label: pnYC-NpH-CeeIF4E3_1-215opt_AB
FEATURES             Location/Qualifiers
     source          1..5081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2294..2313
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2402..2421
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2402..2420
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2421..2445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2460..2482
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2501..2518
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2540..2563
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(3313..3331)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3327..3374
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(3549..3554,3555..3571,3572..3577))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      3689..4234
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     4135..4154
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4351..4368
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4760..4862
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             join(4863..5081,1..441)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFGMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4929..4948)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
       61 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
      121 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
      181 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
      241 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
      301 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
      361 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
      421 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
      481 gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga
      541 cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac
      601 cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt
      661 tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca
      721 gagcctgata aaaacggtta gcgcttcgtt aatacagatg taggtgttcc acagggtagc
      781 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgcttg tttcggcgtg
      841 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ctgccggcac ctgtcctacg
      901 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
      961 cggaaggagc taccggacag cggtgcggac tgttgtaact cagaataaga aatgaggccg
     1021 ctcatggcgt tgactctcag tcatagtatc gtggtatcac cggttggttc cactctctgt
     1081 tgcgggcaac ttcagcagca cgtaggggac ttccgcgttt ccagacttta cgaaacacgg
     1141 aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt
     1201 cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct
     1261 agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg
     1321 cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa
     1381 gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg
     1441 gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg
     1501 caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca
     1561 acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga
     1621 tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat
     1681 ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa
     1741 gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca
     1801 gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
     1861 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
     1921 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
     1981 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
     2041 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac
     2101 gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga
     2161 gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca
     2221 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc
     2281 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg
     2341 tgatgccggc cacgatgcgt ccggcgtaga ggatcagact aagatctcga tcccgcgaaa
     2401 ttaatacgac tcactatagg ggaattgtga gcggataaca attccccact agtaataatt
     2461 ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag
     2521 cagcggtacc ggcagcggcc tggaagttct gttccagggg ccccatatga gcaccagcgt
     2581 tgcagaaaac aaagcactga gcgcaagcgg tgatgttaat gcaagtgatg caagcgttcc
     2641 gcctgaactg ctgacccgtc atccgctgca gaatcgttgg gcactgtggt atctgaaagc
     2701 agatcgtaat aaagaatggg aggattgcct gaaaatggtg agcctgtttg ataccgttga
     2761 agatttttgg agcctgtata accatattca gagtgccggt ggtctgaatt ggggtagcga
     2821 ttattacctg tttaaagaag gcattaaacc gatgtgggaa gatgtgaata atgttcaggg
     2881 tggtcgttgg ctggttgttg ttgataaaca gaaactgcag cgtcgtaccc agctgctgga
     2941 tcattattgg ctggaactgc tgatggccat tgttggtgaa cagtttgatg aatatggcga
     3001 ttacatttgt ggtgccgttg ttaatgttcg tcagaaaggt gataaagtta gcctgtggac
     3061 ccgtgatgca acacgtgatg atgtgaatct gcgtattggt caggttctga aacaaaaact
     3121 gagcattccg gataccgaaa ttctgcgtta tgaagtgcat aaagatagca gcgcacgtac
     3181 cagcagcacc gttaaaccgc gtatttgtct gtaactcgag cttaagcaat tgggatccta
     3241 atagtctaga gctagcccta ggagatccgg ctgctaacaa agcccgaaag gaagctgagt
     3301 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     3361 tgaggggttt tttgctgaaa ggaggaacta tatccggata tcccgcaaga ggcccggcag
     3421 taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat
     3481 gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa
     3541 actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattac aacttatatc
     3601 gtatggggct gacttcaggt gctacatttg aagagataaa ttgcactgaa atcaagaaat
     3661 attttatctg attaataaga tgatcttctt gagatcgttt tggtctgcgc gtaatctctt
     3721 gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc tgagctacca
     3781 actctttgaa ccgaggtaac tggcttggag gagcgcagtc accaaaactt gtcctttcag
     3841 tttagcctta accggcgcat gacttcaaga ctaactcctc taaatcaatt accagtggct
     3901 gctgccagtg gtgcttttgc atgtctttcc gggttggact caagacgata gttaccggat
     3961 aaggcgcagc ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact
     4021 gcctacccgg aactgagtgt caggcgtgga atgagacaaa cgcggccata acagcggaat
     4081 gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccgc cagggggaaa
     4141 cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc
     4201 gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg ccctctcact
     4261 tccctgttaa gtatcttcct ggcatcttcc aggaaatctc cgccccgttc gtaagccatt
     4321 tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga agcggaatat
     4381 atcctgtatc acatattctg ctgacgcacc ggtgcagcct tttttctcct gccacatgaa
     4441 gcacttcact gacaccctca tcagtgccaa catagtaagc cagtatacac tcccctagcg
     4501 ctgatgtccg gcggtgcttt tgccgttacg caccaccccg tcagtagctg aacaggaggg
     4561 acagctgata gaaacagaag ccactggagc acctcaaaaa caccatcata cactaaatca
     4621 gtaagttggc agcatcaccc gacgcacttt gcgccgaata aatacctgtg acggaagatc
     4681 acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc tgggccaact
     4741 tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac cataatgaaa
     4801 taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta
     4861 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
     4921 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
     4981 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
     5041 ttcacattct tgcccgcctg atgaatgctc atccggaatt c
//
