LOCUS       sequence-290842-        8410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmAGO1-L819Y-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Kuzuoglu-Ozturk D, Bhandari D, Huntzinger E, Fauser M, Helms S, 
            Izaurralde E
  TITLE     miRISC and the CCR4-NOT complex silence mRNA targets independently 
            of 43S ribosomal scanning.
  JOURNAL   EMBO J. 2016 Jun 1;35(11):1186-203. doi: 10.15252/embj.201592901. 
            Epub 2016 Mar 23.
  PUBMED    27009120
REFERENCE   2  (bases 1 to 8410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201592901"; journalName: "EMBO J"; date: "2016-06-1- 
            1"; volume: "35"; issue: "11"; pages: "1186-203"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmAGO1-L819Y-siRNAres_AC
FEATURES             Location/Qualifiers
     source          1..8410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2589..2654
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2685..2711
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5695..5736
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5746..5763
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5786..5803)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5836..5970
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5860..5879)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5914..5933
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6592..7180)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6672..6691)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7351..8142,8143..8211))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7974..7993
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8212..8316)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8384..8402
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacccaacat ggacgcacaa acacgacgac gtgagcgtcg cgctgagaaa caagctcaat
     2641 ggaaagctgc aaacggtgga tctcctccaa gcttgaattc tatgtaccca tacgatgttc
     2701 cagattacgc tatgtccacg gagcgtgagc tggctcccgg tgggccagct cagctccatc
     2761 cgcacacgct gccgctgact tttccggatc tccagatgac ctccaccgtg ggcatcattg
     2821 ggaaagtcta cgagtcacag tggaccccct cgcccactcg gcctcagagt ccctctcagg
     2881 cgcagactag cttcgacacg ctcacatcac caccagcgcc cggctcgtcg gtcaatccca
     2941 ccgccgtcac cagcccaagt gcccagaatg tggccgctgg tggagcaact gtggccggtg
     3001 cagcagcaac tgccgcccag gtggcctccg ccttgggtgc caccaccggc agcgtgacgc
     3061 cagcaattgc caccgcaacg ccagccacgc agccggatat gcccgtcttt acgtgtccac
     3121 gacgcccgaa tctcggacga gagggtcgcc cgattgtgct gcgcgccaat cacttccagg
     3181 tgacaatgcc gcgtggctat gtgcatcact atgacatcaa tatacagccg gacaagtgtc
     3241 cgcgaaaggt gaaccgtgag attatcgaga ctatggtgca tgcctatagc aagatcttcg
     3301 gagtgctcaa gccggtgttc gatggtcgca acaatctgta tacccgcgat cccctgccca
     3361 ttggcaacga gcgtctagag ctggaggtta ctctacccgg cgagggcaaa gatcgaatct
     3421 ttcgcgtgac gatcaagtgg caggctcagg tctcgctctt caatttggag gaagctctcg
     3481 aaggccgcac gcggcagata ccctatgatg ccattttggc gctcgatgtg gtcatgcgcc
     3541 atctgcccag catgacgtac acgccagtgg gacgtagctt cttcagttcc ccggagggtt
     3601 actaccatcc cctgggtggt ggacgcgagg tttggttcgg tttccatcag agcgcaaggc
     3661 cctcgcagtg gaagatgatg ctcaatatcg atgtctcggc caccgctttc tacaaggctc
     3721 aaccagtcat tgacttcatg tgcgaggtgc tggacattcg cgacatcaac gagcagcgca
     3781 aaccgctcac cgattcgcag cgcgtcaagt tcaccaaaga aattaaaggc ctgaagatcg
     3841 agatcaccca ctgcggccag atgcgtcgca agtatcgtgt gtgcaacgtc actcgccgcc
     3901 ccgctcagat gcaatcattc ccactgcagc tggagaacgg acagaccgta gagtgcaccg
     3961 tggccaagta cttcctggac aagtaccgca tgaaattgcg ctacccgcac ttgccctgcc
     4021 tgcaggttgg ccaagagcac aagcacactt acctgcctct agaggtgtgc aacattgtgg
     4081 ccggacagcg gtgcattaaa aagctgaccg atatgcagac gtcgaccatg atcaaggcca
     4141 cagctcgttc agctccggat cgtgagcgtg agattaacaa cttggtaaag cgcgccgact
     4201 tcaacaacga ttcgtatgtg caagagtttg gcctgaccat ctccaattcg atgatggagg
     4261 tacgaggacg cgtcttgcct cctcccaagc ttcagtatgg gggacgtgtg tctaccggcc
     4321 tcaccggcca gcagctgttc ccgccacaga acaaggtgag cttggcctcg cccaaccagg
     4381 gtgtatggga tatgcgaggc aagcagttct tcactggcgt cgagatccgc atctgggcca
     4441 tcgcctgttt tgccccacag cgcacggtgc gcgaggatgc gctgcgtaat ttcacccagc
     4501 agctgcagaa gatctcaaac gatgcaggca tgccgataat tggacagccg tgcttctgta
     4561 agtacgccac cgggccggat caagtggaac ccatgttccg ttacctgaag atcaccttcc
     4621 ccggcctgca actcgtcgtg gttgtgctgc ccggcaagac tccagtatac gccgaggtga
     4681 agcgtgtagg tgacaccgtt ctgggtatgg ccacccagtg tgtgcaggcc aagaacgtga
     4741 acaagacatc gccacagacg ctctctaatc tgtgtctgaa gatcaacgtc aagttgggcg
     4801 gcatcaattc aattctggtc ccctccattc ggccaaaggt cttcaatgag ccggtcatct
     4861 ttttgggtgc cgatgtgaca cacccaccag ctggcgacaa caagaaacca tcgattgccg
     4921 ccgtcgtggg ctccatggat gcccatccat cgcggtatgc cgccaccgtt cgcgtacagc
     4981 agcaccgaca ggagatcatc caggagctga gcagcatggt gcgcgagctg ttgatcatgt
     5041 tctacaagtc gacgggcggc tacaagcccc accgcatcat actctatcgt gacggagtct
     5101 ccgagggaca attcccacat gtcctgcaac acgaattgac cgccattcgc gaggcctgca
     5161 ttaagtatga gccagagtat cggccgggca tcacattcat tgtggtgcag aagcgccatc
     5221 acactcgact cttctgcgcg gagaagaagg agcagagcgg caaatcgggc aatattcccg
     5281 caggcaccac cgtcgatgtg ggcatcacac atcccaccga atttgatttc tatctatgca
     5341 gccatcaggg catccagggc accagtcgcc cctcgcacta ccacgttctg tgggacgaca
     5401 atcactttga ctcggacgag ctgcagtgcc tcacgtatca gctatgccgt acgtacgtgc
     5461 gctgcacccg atccgtcagt ataccggcgc cagcctacta cgcccattta gtggccttcc
     5521 gtgccagata tcatctggtg gagaaggagc acgattcggg cgagggttcg caccagagcg
     5581 gctgctcaga ggatcgtacg ccaggtgcca tggccagggc catcactgtg cacgcggata
     5641 ccaagaaggt catgtacttt gcctaactcg agtctagagg gcccgcggtt cgaaggtaag
     5701 cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac
     5761 cattgagttt aaacccgctg atcagcctcg actgtgcctt ctaaggcctg agctcgctga
     5821 tcagcctcga tcgaggatcc agacatgata agatacattg atgagtttgg acaaaccaca
     5881 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     5941 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     6001 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     6061 atggctgatt atgatcagtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     6121 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     6181 actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg
     6241 gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca
     6301 agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg
     6361 catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc
     6421 cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat
     6481 gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct
     6541 taccagccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6601 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6661 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6721 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6781 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6841 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6901 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6961 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7021 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7081 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7141 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7201 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7261 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7321 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7381 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7441 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7501 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7561 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7621 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt
     7681 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7741 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7801 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7861 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7921 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
     7981 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8041 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8101 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8161 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8221 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8281 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8341 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     8401 gccctttcgt
//
