LOCUS       sequence-290847-        7905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot1_1149-1962-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Kuzuoglu-Ozturk D, Bhandari D, Huntzinger E, Fauser M, Helms S, 
            Izaurralde E
  TITLE     miRISC and the CCR4-NOT complex silence mRNA targets independently 
            of 43S ribosomal scanning.
  JOURNAL   EMBO J. 2016 Jun 1;35(11):1186-203. doi: 10.15252/embj.201592901. 
            Epub 2016 Mar 23.
  PUBMED    27009120
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201592901"; journalName: "EMBO J"; date: "2016-06-1- 
            1"; volume: "35"; issue: "11"; pages: "1186-203"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmNot1_1149-1962-dsRNAres_AC
FEATURES             Location/Qualifiers
     source          1..7905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5190..5231
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5241..5258
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5281..5298)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5331..5465
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5355..5374)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5409..5428
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6087..6675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6167..6186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6846..7637,7638..7706))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7469..7488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7707..7811)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7879..7897
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttaatta aggtgccacc agagcctgtg caggacaaaa ctgcctttat
     2761 tttcaacaac ttgagccagc tgaacatacc gcagaagtgc gatgagataa aggagattat
     2821 gaccaaggag tattggccgt ggctagcgca atatctggtc ctcaaacgcg catcaatgga
     2881 gttcaacttc cacacactat attacaactt tttggatgcc cttaaaaacg gcgagatcaa
     2941 ccgattcgta accaaagaga ccctacgcaa catcaaggta ctcttgcgct ccgataaggg
     3001 agttattaat ttctccgatc gaagtctgtt gaagaacctc ggacactggt tgggcatgat
     3061 gaccttaggt cgcaatcgcc ccatcctgca gttggatctg gatcttaaat cccttttggc
     3121 tgaggcttac cacaagggcc agcaggaact gctttttgtg gttcctttcg tagccaagat
     3181 ccttgagtca tccgccaagt ctcgcatctt ccgatcgccc aatccatgga caatgggtat
     3241 catgtacgtg cttgctgagc ttcaccagga gccagacctt aagctgaacc tgaagtttga
     3301 aatcgaagta ctctgcaaaa cacttaatct ggagttggcc aagttgcgac aagtgatcta
     3361 cctgaaggat cccaatcgaa ctcaccggat tgaagagcag atgtcgcaac caaaaccgaa
     3421 acaacttgag ccagtggctt ctgcgccagc tctaccgcgt gaacagcaat ccccggcaca
     3481 acctccgcca ccaccacaac agcagcaacc accgcagcag caggtacctc caccgccatc
     3541 ctctgcggac gtggacgccc aaaatgcggc cgctatgatg atggcagcgg gcggagctaa
     3601 tagtactcct ggatcggtat cttcgcccaa tctacccacc gattccagcc aggtggcact
     3661 accgccacca gagccgcgct attcatacgt ggacgtaaac gtgagcaact tccagcttat
     3721 tggtcagcag ttggtactgc cacccaatac tccgttcctg cacgccaatc ccggaatcaa
     3781 acacatggtc gtcaacgccg tggagcgcac gataaccgac tggttgcagc ctattgtgga
     3841 ccgaagcatc cgcattgcct gtgccaccac cgagcagatc atccgcaagg acttcgccct
     3901 ggatgctgac gagaaccgaa tgcgcactgc cgctcatcag atggtacgaa accttgccgc
     3961 cggcatggcc atgattaccg gtaaggatga gatagcgcgt gctattagcc agaatctgca
     4021 caaggccttg ctgtcgggtc taaatgggat gcccagcatg gctgagatcc aagctgctgc
     4081 tatgcagtta gccagcgaaa atgttgagct agtttgcgct ttcattcaga aaacatctgc
     4141 tgaaaaggca gccgcagaga tcgataggcg tctaagcacc gatttcgaga ccagaaaaat
     4201 tgctcgtgaa gagggcaacc gctttgtgga cgcccagatc ctcacctatc aacaggagcg
     4261 tcttccagaa gcagtgcgca tcaaggtggg cgcggctcca gctacacttt atgctgtgta
     4321 ctcggagttc gctagaagca tacctggctt ccagcagatg agcgatcgcg atattgccct
     4381 gtttgtgccc aagccgacgg atttgtctca gccaaatgtg tttgctaatg atgatagcat
     4441 ggtatacggg gaactggcta gcaaaatgga ggccttcatg aacacggcaa tcggagtgcc
     4501 gacactccag atccaggcta gcaagatgca catgctactc aacgccctga tagcgacgcg
     4561 tcgactgcgt gaccaagagt ctgcctttaa tctgctgacc cgcgcggtcg agggtttgac
     4621 cgaaggattg gtcaatatgc atgaaaacat ggagcaaatg aagatgtacc aaaacatcca
     4681 tctccgtatc cttggcttgc tgaacaacag tttcggtgct ccaaacacag agcgagcggt
     4741 aacaaagtgt ttcttcgata tccgagagga ggtgcgatat aacgtggagg cagctcgcgc
     4801 tttgattacg tcgcactttg tcaatctgaa tcagtttgac ggaatgctac gcgactgcat
     4861 ggacaacgga aacaattatg tggctatatc gttcggtatc gcactgctgg agcgcctcat
     4921 catggacgat cgagtgatta atattgtttc ggacaacgaa ttcatggcaa ctgttgagct
     4981 gctaggtagg ctgacccaac accgtcaccg ctaccccgag tgtatcgtca acgccatcga
     5041 caccctgtgg tccggcaatt tcaatagctc ctccgattat tcccccttta atggaaacga
     5101 ccgctatctg agcggcgcta gccactatat tcatagcgga atgcaccatg tccgctcgtg
     5161 tgactaatct agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc
     5221 tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag
     5281 cctcgactgt gccttctaag gcctgagctc gctgatcagc ctcgatcgag gatccagaca
     5341 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     5401 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     5461 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     5521 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cagtcgaccg
     5581 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
     5641 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
     5701 ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg
     5761 cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc
     5821 aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac
     5881 gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct
     5941 tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac
     6001 catcagggac agcttcaagg atcgctcgcg gctcttacca gccagcaaaa ggccaggaac
     6061 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6121 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6181 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6241 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     6301 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6361 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6421 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6481 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     6541 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6601 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6661 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6721 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6781 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6841 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6901 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6961 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7021 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7081 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7141 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7201 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7261 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7321 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7381 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7441 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     7501 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7561 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7621 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7681 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7741 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7801 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     7861 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgt
//
