LOCUS       sequence-290890-       11280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11280)
  AUTHORS   Kuzuoglu-Ozturk D, Bhandari D, Huntzinger E, Fauser M, Helms S, 
            Izaurralde E
  TITLE     miRISC and the CCR4-NOT complex silence mRNA targets independently 
            of 43S ribosomal scanning.
  JOURNAL   EMBO J. 2016 Jun 1;35(11):1186-203. doi: 10.15252/embj.201592901. 
            Epub 2016 Mar 23.
  PUBMED    27009120
REFERENCE   2  (bases 1 to 11280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201592901"; journalName: "EMBO J"; date: "2016-06-1- 
            1"; volume: "35"; issue: "11"; pages: "1186-203"
COMMENT     Sequence Label: pLNHA-C1-HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNA...
FEATURES             Location/Qualifiers
     source          1..11280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             665..730
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             755..781
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    8068..8189
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8105..8124)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8159..8178
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8196..8651)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8333..8354)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8545..8564
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8678..8782
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8784..9141
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8784..8975
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8807..8827)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8992..9127
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9176..9970
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9176..9970
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9230..9249)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9840..9859
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10158..10177)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10202..10249
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10578..11166
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11067..11086
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
      721 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
      781 tcctccatct cgagctatga atcttgactc gctctcgctg gccttgtctc aaatcagcta
      841 cctggtggac aatttaacca agaaaaatta ccgagccagc cagcaggaaa tacagcatat
      901 tgtgaatcgg cacggtcctg aggcagacag gcatttatta cgctgcctat tttcgcatgt
      961 ggatttcagt ggcgatggta aaagcagtgg caaagatttc catcagactc agtttctgat
     1021 tcaggagtgt gcgttgctga ttacaaagcc aaattttatc tcgacgctgt cctatgccat
     1081 tgataatcca ttgcactatc agaagagttt aaagcctgca ccccacttat ttgcccagct
     1141 gagtaaagtg ctcaaattaa gcaaagtaca agaggtaatt tttggccttg ccctgttgaa
     1201 ttcttccagc tcagatctta gaggtttcgc tgcccagttt atcaaacaga agcttccaga
     1261 tcttctgcgt tcttacattg acgcagacgt cagtggaaat caagaaggtg gcttccaaga
     1321 tatagcaata gaggtcctac acctcctcct ctcccatctc ctctttgggc agaagggagc
     1381 ctttggagtt ggacaagaac agatagacgc ttttcttaag acgctgcgca gagattttcc
     1441 ccaagaacgc tgtcccgtgg tgctcgcacc acttttatac cctgaaaaac gggacattct
     1501 aatggacagg atcctgcctg attccggagg ggtagctaaa accatgatgg agagctcttt
     1561 ggctgatttc atgcaagaag taggctatgg cttttgtgca agtattgaag aatgtcgcaa
     1621 tataatcgtg cagtttggtg ttcgggaggt cacagctgcc caggttgcaa gggttttggg
     1681 aatgatggct cgaactcatt caggattaac agatggcatt ccattacaga gtatttctgc
     1741 tccgggcagt gggatctgga gtgatgggaa agataaaagt gatggagcac aggcacacac
     1801 atggaatgta gaagtcttga ttgacgttct taaagaactg aatccaagtt tgaatttcaa
     1861 ggaagtaact tatgaactgg accatcctgg atttcaaatt cgtgacagta aaggacttca
     1921 taatgtggtt tatggcattc agaggggttt gggtatggaa gtgttcccag tagacctcat
     1981 atatagacct tggaaacatg ctgaaggcca gctctccttc atccagcact cactaatcaa
     2041 tccagagatc ttctgttttg ctgactatcc ctgtcatact gttgccactg atattctgaa
     2101 agcaccacca gaggatgaca atcgagaaat tgccacatgg aagagcttgg atttgattga
     2161 atctctgctg aggcttgcag aggttgggca gtatgagcaa gtcaaacagc tcttcagctt
     2221 ccctatcaaa cactgtccag acatgctggt attggcctta ctacaaatta acacctcttg
     2281 gcataccttg cgccatgaac ttatctccac tctgatgcca attttccttg gaaaccatcc
     2341 taactcagct attattttgc actatgcatg gcatgggcag ggacagtctc cctcaattcg
     2401 ccaacttatc atgcatgcaa tggcagaatg gtacatgaga ggggagcagt atgatcaggc
     2461 caaattgtct cgaatacttg atgtggccca ggacttgaag gccttgtcaa tgctgctaaa
     2521 tggtactcca tttgcctttg ttattgacct tgctgcactt gcttcacgtc gtgaatacct
     2581 caaacttgat aagtggctca cagataaaat tcgagagcat ggggagcctt ttatccaggc
     2641 gtgtatgact tttttaaaga gacggtgtcc ttctattttg ggcggacttg ccccagaaaa
     2701 agaccagccc aaaagtgctc aacttcctcc agaaactttg gcgacaatgt tggcctgtct
     2761 gcaagcttgt gcagggagtg tttctcagga gctatcagaa actatcctca ccatggtagc
     2821 caattgcagt aatgttatga ataaggccag acaaccacca cctggagtta tgccaaaagg
     2881 acgtcctcct agtgctagca gcttagatgc catttctcct gttcagattg accctcttgc
     2941 tggaatgaca tctcttagta taggtggttc agctgcccct cacacccaga gtatgcaggg
     3001 ttttcctcca aatttgggtt ctgcattcag tacccctcag tcaccagcaa aagcatttcc
     3061 acccctttca acccccaatc agaccactgc attcagtggt attggaggac tttcatcaca
     3121 gcttccagta ggtggtcttg gcacaggcag cctgactggt ataggaactg gtgctcttgg
     3181 actccctgca gtgaataacg acccttttgt acagaggaaa ctgggcacct ctggactgaa
     3241 tcagcctaca ttccagcaga gtaagatgaa accttcggac ttgtctcagg tgtggccaga
     3301 ggcaaaccag cactttagta aagagataga tgatgaagca aacagctatt tccagcgaat
     3361 atataatcat ccaccacatc caaccatgtc tgttgatgag gtattagaaa tgctgcagag
     3421 atttaaagac tctactataa agagggaacg agaagtattt aactgtatgc taaggaactt
     3481 gtttgaagaa tatcgttttt ttccccagta tcctgataaa gagttacata taacagcctg
     3541 cctatttggt ggtataattg agaaaggact ggtcacttac atggcactag gtctggctct
     3601 acgatatgtt cttgaagcct tacgcaagcc ttttggatcc aaaatgtatt atttcgggat
     3661 tgctgcacta gatagattta aaaacagatt gaaggactat ccccagtatt gtcagcattt
     3721 ggcttctatc agtcacttta tgcaatttcc acatcattta caggagtata ttgagtatgg
     3781 acagcagtct agagatcctc ctgtgaaaat gcaaggctct atcacaaccc ctggaagtat
     3841 tgcactggct caggcccagg ctcaggccca ggttccagca aaagctcctc ttgctggtca
     3901 agttagcact atggtaacca cctcaacaac taccactgtt gctaaaacgg ttacggtcac
     3961 caggccaact ggagtcagct ttaagaaaga tgtgccacct tctattaata ctacaaatat
     4021 agatacgttg cttgtggcca cagatcaaac tgagagaatt gtggagcccc cagaaaatat
     4081 ccaggagaaa attgctgcta ttttcaatgc tctctcacag tcaaatatga cacaagcggt
     4141 tgaagagcta aaggaaacgg tgaaagaaga atttatgcct tgggtttcac agtatctggt
     4201 tatgaaggca gtcagtattg agccagccgc tcatagcctg tattcaaact tccttgacac
     4261 gctgaagaat cctgaattta acaagatggt tctgaatgag acctacagaa acattaaagt
     4321 gctcctgacc tctgataaag ctgcagccaa tttctcagat cgttctttgc tgaagaactt
     4381 gggacattgg ctaggaatga tcacattagc taaaaacaaa cccatcttac acactgactt
     4441 ggatgtgaaa tcattgctgc tagaggctta tgttaaagga caacaagaat tgctctatgt
     4501 agtgcccttt gttgccaaag tcttagaatc tagcattcgt agtgtggttt ttaggccacc
     4561 aaacccttgg acaatggcaa ttatgaatgt attagctgag ctacatcagg agcatgactt
     4621 agcgttaaac ttgaaggctg aaatcgcggt tctctgcaag aaccttgcat tagacatcaa
     4681 tgagctaaaa cctggaaacc tcctaaagga taaagatcgc ctgaagaatt tagatgagca
     4741 actctctgct ccaaagaaag atgtcaagca gccagaagaa ctccctccca tcacaaccac
     4801 aacaacttct actacaccag ctaccaacac cacttgtaca gccacggttc caccacagcc
     4861 acagtacagc taccacgaca tcaatgtcta ttcccttgcg ggcttggcac cacacattac
     4921 tctaaatcca acaattccct tgtttcaggc ccatccacag ttgaagcagt gtgtgcgtca
     4981 ggcaattgaa cgggctgtcc aggagctggt ccatcctgtg gtggatcgat caattaagat
     5041 tgccatgact acttgtgagc aaatagtcag gaaggatttt gccctggatt cggaggaatc
     5101 tcgaatgcga atagcagctc atcacatgat gcgtaacttg acagctggaa tggctatgat
     5161 tacatgcagg gaacctttgc tcatgagcat atctaccaac ttaaaaaaca gttttgcctc
     5221 agcccttcgt actgcttccc cacaacaaag agaaatgatg gatcaggcag ctgctcaatt
     5281 agctcaggac aattgtgagt tggcttgctg ttttattcag aagactgcag tagaaaaagc
     5341 aggccctgag atggacaaga gattagcaac tgaatttgag ctgagaaaac atgctaggca
     5401 agaaggacgc agatactgtg atcctgttgt tttaacatat caagctgaac ggatgccaga
     5461 gcaaatcagg ctgaaagttg gtggtgtgga cccaaagcag ttggctgttt acgaagagtt
     5521 tgcacgcaat gttcctggct tcttacctac aaatgactta agtcagccca cgggattttt
     5581 agcccagccc atgaagcaag cttgggcaac agatgatgta gctcagattt atgataagtg
     5641 tattacagaa ctggagcaac atctacatgc catcccacca actttggcca tgaaccctca
     5701 agctcaggct cttcgaagtc tcttggaggt tgtagtttta tctcgaaact ctcgggatgc
     5761 catagctgct cttggattgc tccaaaaggc tgtagagggc ttactagatg ccacaagtgg
     5821 tgctgatgct gaccttctgc tgcgctacag ggaatgccac ctcttggtcc taaaagctct
     5881 gcaggatggc cgggcatatg ggtctccatg gtgcaacaaa cagatcacaa ggtgcctaat
     5941 tgaatgtcga gatgaatata aatataatgt ggaggctgtg gagctgctaa ttcgcaatca
     6001 tttggttaat atgcagcagt atgatcttca cctagcgcag tcaatggaga atggcttaaa
     6061 ctacatggct gtggcatttg ctatgcagtt agtaaaaatc ctgctggtgg atgaaaggag
     6121 tgttgctcat gttactgagg cagatctgtt ccacaccatt gaaaccctca tgaggattaa
     6181 tgctcattcc agaggcaatg ctccagaagg attgccccag ctgatggaag tagtgcgatc
     6241 caactatgaa gcaatgattg atcgtgctca tggaggccca aactttatga tgcattctgg
     6301 gatctctcaa gcctcagagt atgatgaccc tccaggcctg agggagaagg cagagtatct
     6361 tctgagggaa tgggtgaatc tctaccattc agcagcagct ggccgcgaca gtaccaaagc
     6421 tttctctgca tttgttggac agatgcacca gcaaggaata ctgaagaccg atgatctcat
     6481 aacaaggttc tttcgtctgt gtactgaaat gtgtgttgaa atcagttacc gtgctcaggc
     6541 tgagcagcag cacaatcctg ctgccaatcc caccatgatc cgagccaagt gctatcacaa
     6601 cctggatgcc tttgttcgac tcattgcact gctcgtgaaa cactcagggg aggccaccaa
     6661 cactgtcaca aagattaatc tgctgaacaa ggtccttggt atagtagtgg gagttctcct
     6721 tcaggatcat gatgttcgtc agagtgaatt tcagcaactt ccctaccatc gaatttttat
     6781 catgcttctc ttggaactca atgcacctga gcatgtgttg gaaaccatta atttccagac
     6841 acttacagct ttctgcaata cattccacat cttgaggcct accaaagctc ctggctttgt
     6901 atatgcctgg cttgaactga tttcccatcg gatatttatt gcaagaatgc tggcacatac
     6961 gccacagcag aaggggtggc ctatgtatgc acagctactg attgatttat tcaaatattt
     7021 agcgcctttc cttagaaatg tggaactcac caaacctatg caaatcctct acaagggcac
     7081 tttaagagtg ctgctggttc ttttgcatga tttcccagag ttcctttgtg attaccatta
     7141 tgggttctgt gatgtgatcc cacctaattg tatccagtta agaaatttga tcctgagtgc
     7201 ctttccaaga aacatgaggc tccccgaccc attcactcct aatctaaagg tggacatgtt
     7261 gagtgaaatt aacattgctc cccggattct caccaatttc actggagtaa tgccacctca
     7321 gttcaaaaag gatttggatt cctatcttaa aactcgatca ccagtcactt tcctgtctga
     7381 tctgcgcagc aacctacagg tatccaatga acctgggaat cgctacaacc tccagctcat
     7441 caatgcactg gtgctctatg tcgggactca ggccattgcg cacatccaca acaagggcag
     7501 cacaccttca atgagcacca tcactcactc agcacacatg gatatcttcc agaatttggc
     7561 tgtggacttg gacactgagg gtcgctatct ctttttgaat gcaattgcaa atcagctccg
     7621 gtacccaaat agccacactc actacttcag ttgcaccatg ctgtaccttt ttgcagaggc
     7681 caatacggaa gccatccaag aacagatcac aagagttctc ttggaacggt tgattgtaaa
     7741 taggccacat ccttggggtc ttcttattac cttcattgag ctgattaaaa acccagcgtt
     7801 taagttctgg aaccatgaat ttgtacactg tgccccagaa atcgaaaagt tattccagtc
     7861 ggtcgcacag tgctgcatgg gacagaagca ggcccagcaa gtaatggaag ggacaggtgc
     7921 cagttagccg cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc
     7981 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     8041 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     8101 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     8161 ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt
     8221 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     8281 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     8341 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     8401 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     8461 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     8521 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     8581 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     8641 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     8701 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     8761 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     8821 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     8881 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     8941 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     9001 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     9061 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     9121 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
     9181 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     9241 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     9301 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     9361 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     9421 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     9481 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     9541 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     9601 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     9661 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     9721 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     9781 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     9841 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     9901 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     9961 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
    10021 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
    10081 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
    10141 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
    10201 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
    10261 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
    10321 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
    10381 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
    10441 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    10501 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    10561 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    10621 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    10681 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
    10741 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    10801 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    10861 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    10921 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    10981 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    11041 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    11101 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    11161 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    11221 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
