LOCUS       sequence-290925-        6346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of A60-HhR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6346)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1C-RLuc-V5His6-A90-HhR_AD
FEATURES             Location/Qualifiers
     source          1..6346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     29..47
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        111..2623
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2668..3600
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKTEQ"
     CDS             3622..3663
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3673..3690
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3713..3730)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(3787..3806)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3841..3860
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_RNA        3990..4041
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     rep_origin      complement(4583..5171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4663..4682)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5342..6133,6134..6202))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5965..5984
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6203..6307)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
       61 aggaattaat tctatattct aaaaacacaa atgatacttc taaaaaaaat catgaatggc
      121 atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact gtcacatcat
      181 ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt tatggccatt
      241 aaagctggct gatcgtcgcc aaacaccaaa tacatatcaa tatgtacatt cgagaaagaa
      301 gcgatcaaag aagcgtcttc gggcgagtag gagaatgcgg aggagaagga gaacgagctg
      361 atctagtatc tctccacaat ccaatgccaa ctgaccaact ggccatattc ggagcaattt
      421 gaagccaatt tccatcgcct ggcgatcgct ccattcttgg ctatatgttt ttcaccgttc
      481 ccggggccat tttcaaagac tcgtcggtaa gataagattg tgtcactcgc tgtctctctt
      541 catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt tgaagaatga
      601 gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa catgttatct
      661 atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta tataccttta
      721 atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg atattttaag
      781 atgatgccag accacaggct gatttctgcg tcttttgccg aacgcagtgc atgtgcggtt
      841 gttgtttttt ggaatagttt caattttcgg actgtccgct ttgatttcag tttcttggct
      901 tattcaaaaa gcaaagtaaa gccaaaaaag cgagatggca ataccaaatg cggcaaaacg
      961 gtagtggaag gaaaggggtg cggggcagcg gaaggaaggg tggggcgggg cgtggcgggg
     1021 tctgtggctg ggcgcgacgt caccgacgtt ggagccactc ctttgaccat gtgtgcgtgt
     1081 gtgtattatt cgtgtctcgc cactcgccgg ttgttttttt ctttttatct cgctctctct
     1141 agcgccatct cgtacgcatg ctcaacgcac cgcatgttgc cgtgtccttt atgcgtcatt
     1201 ttggctcgaa ataggcaatt atttaaacaa agattagtca acgaaaacgc taaaataaat
     1261 aagtctacaa tatggttact tattgccatg tgtgtgcagc caacgatagc aacaaaagca
     1321 acaacacagt ggctttccct ctttcacttt ttgtttgcaa gcgcgtgcga gcaagacggc
     1381 acgaccggca aacgcaatta cgctgacaaa gagcagacga agttttggcc gaaaaacatc
     1441 aaggcgcctg atacgaatgc atttgcaata acaattgcga tatttaatat tgtttatgaa
     1501 gctgtttgac ttcaaaacac acaaaaaaaa aaataaaaca aattatttga aagagaatta
     1561 ggaatcggac agcttatcgt tacgggctaa cagcacaccg agacgaaata gcttacctga
     1621 cgtcacagcc tctggaagaa ctgccgccaa gcagagagag agagaaaaag agggagagca
     1681 gcttagaccg catgtgcttg tgtgtgaggc gtctctctct tcgtctcctg tttgcgcaaa
     1741 cgcatagact gcactgagaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1801 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1861 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1921 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1981 aaaaccggct taagttaggt gtgttttcta cgactagtga tgcccctact agaagatgtg
     2041 tgttgcacaa atgtccctga ataaccaatt tgaagtgcag atagcagtaa acgtaagcta
     2101 atatgaatat tatttaactg taatgtttta atatcgctgg acattactaa taaacccact
     2161 ataaacacat gtacatatgt atgttttggc atacaatgag tagttgggga aaaaatgtgt
     2221 aaaagcaccg tgaccatcac agcataaaga taaccagctg aagtatcgaa tatgagtaac
     2281 ccccaaattg aatcacatgc cgcaactgat aggacccatg gaagtacact cttcatggcg
     2341 atatacaaga cacacacaag cacgaacacc cagttgcgga ggaaattctc cgtaaatgaa
     2401 aacccaatcg gcgaacaatt catacccata tatggtaaaa gttttgaacg cgacttgaga
     2461 gcggagagca ttgcggctga taaggtttta gcgctaagcg ggctttataa aacgggctgc
     2521 gggaccagtt ttcatatcac taccgtttga gttcttgtgc tgtgtggata ctcctcccga
     2581 cacaaagccg ctccatcagc cagcagtcgt ctaatccaga gaccccggat cggggtacct
     2641 actagtccag tgtggtggaa ttccaacatg acttcgaaag tttatgatcc agaacaaagg
     2701 aaacggatga taactggtcc gcagtggtgg gccagatgta aacaaatgaa tgttcttgat
     2761 tcatttatta attattatga ttcagaaaaa catgcagaaa atgctgttat ttttttacat
     2821 ggtaacgcgg cctcttctta tttatggcga catgttgtgc cacatattga gccagtagcg
     2881 cggtgtatta taccagacct tattggtatg ggcaaatcag gcaaatctgg taatggttct
     2941 tataggttac ttgatcatta caaatatctt actgcatggt ttgaacttct taatttacca
     3001 aagaagatca tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca ttatagctat
     3061 gagcatcaag ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga tgtgattgaa
     3121 tcatgggatg aatggcctga tattgaagaa gatattgcgt tgatcaaatc tgaagaagga
     3181 gaaaaaatgg ttttggagaa taacttcttc gtggaaacca tgttgccatc aaaaatcatg
     3241 agaaagttag aaccagaaga atttgcagca tatcttgaac cattcaaaga gaaaggtgaa
     3301 gttcgtcgtc caacattatc atggcctcgt gaaatcccgt tagtaaaagg tggtaaacct
     3361 gacgttgtac aaattgttag gaattataat gcttatctac gtgcaagtga tgatttacca
     3421 aaaatgttta ttgaatcgga cccaggattc ttttccaatg ctattgttga aggtgccaag
     3481 aagtttccta atactgaatt tgtcaaagta aaaggtcttc atttttcgca agaagatgca
     3541 cctgatgaaa tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa aactgaacaa
     3601 gctcgaggtc acccattcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
     3661 acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact
     3721 gtgccttcta aggcctgagc tcgctgatca gcctcgatcg aggatccaga catgataaga
     3781 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     3841 gaaatttgtg atgctattgc tgcggccgca tacaaaggat ctggctgcta gataaaaaaa
     3901 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3961 aaaaaaaaaa aaaaaaaaaa aaaaaaaaac ctgtcaccgg atgtgttttc cggtctgatg
     4021 agtccgtgag gacgaaacag ggaattcgat atcaagctta tcgataccgt cgaccgatgc
     4081 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
     4141 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     4201 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     4261 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
     4321 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     4381 tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     4441 gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     4501 agggacagct tcaaggatcg ctcgcggctc ttaccagcca gcaaaaggcc aggaaccgta
     4561 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4621 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4681 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4741 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
     4801 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4861 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4921 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4981 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     5041 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     5101 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     5161 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5221 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     5281 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     5341 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5401 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5461 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5521 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5581 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5641 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5701 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     5761 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     5821 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5881 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5941 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6001 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6061 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6121 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6181 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6241 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6301 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaac
//
