LOCUS       sequence-290948-        7848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot1_1710-2505-H2067D-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Raisch T, Bhandari D, Sabath K, Helms S, Valkov E, Weichenrieder O, 
            Izaurralde E
  TITLE     Distinct modes of recruitment of the CCR4-NOT complex by Drosophila 
            and vertebrate Nanos.
  JOURNAL   EMBO J. 2016 May 2;35(9):974-90. doi: 10.15252/embj.201593634. Epub 
            2016 Mar 11.
  PUBMED    26968986
REFERENCE   2  (bases 1 to 7848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201593634"; journalName: "EMBO J"; date: "2016-05-2- 
            2"; volume: "35"; issue: "9"; pages: "974-90"
COMMENT     Sequence Label: 
            pAc5.1B-lambdaN-HA-DmNot1_1710-2505-H2067D-dsRNAres_AD
FEATURES             Location/Qualifiers
     source          1..7848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5133..5174
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5184..5201
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5224..5241)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5274..5408
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5298..5317)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5352..5371
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6030..6618)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6110..6129)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6789..7580,7581..7649))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7412..7431
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7650..7754)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7822..7840
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttaatta agcagccaaa tgtgtttgct aatgatgata gcatggtata
     2761 cggggaactg gctagcaaaa tggaggcctt catgaacacg gcaatcggag tgccgacact
     2821 ccagatccag gctagcaaga tgcacatgct actcaacgcc ctgatagcga cgcgtcgact
     2881 gcgtgaccaa gagtctgcct ttaatctgct gacccgcgcg gtcgagggtt tgaccgaagg
     2941 attggtcaat atgcatgaaa acatggagca aatgaagatg taccaaaaca tccatctccg
     3001 tatccttggc ttgctgaaca acagtttcgg tgctccaaac acagagcgag cggtaacaaa
     3061 gtgtttcttc gatatccgag aggaggtgcg atataacgtg gaggcagctc gcgctttgat
     3121 tacgtcgcac tttgtcaatc tgaatcagtt tgacggaatg ctacgcgact gcatggacaa
     3181 cggaaacaat tatgtggcta tatcgttcgg tatcgcactg ctggagcgcc tcatcatgga
     3241 cgatcgagtg attaatattg tttcggacaa cgaattcatg gcaactgttg agctgctagg
     3301 taggctgacc caacaccgtc accgctaccc cgagtgtatc gtcaacgcca tcgacaccct
     3361 gtggtccggc aatttcaata gctcctccga ttattccccc tttaatggaa acgaccgcta
     3421 tctgagcggc gctagccact atattcatag cggaatgcac catgtccgct cgtgtgacac
     3481 cgacgacccc cccggactcc aggaaaaaac cgaattcttg ttgaaagact gggtggcctt
     3541 gtatacccag caaaatcaac aatcgacccg tgacgcccgt aattttggcg ccttcgtgca
     3601 aaaaatgaat acctacggca ttttgaagac cgatgatctg attacgcgct tctttcgtca
     3661 agctacccat atctgtaccg acgtcgtcta tcgtatgttt gctgaaccca gccttcccat
     3721 taatcaagct aagaataaaa tttttcaatg gattgacgcc tttgtgcact tgatcgccat
     3781 gctggtccgc gactcgggag aggccggcaa ccccacgacg aaaattaatt tgctcaataa
     3841 agtcttggga atcgtcttgg gcaccttgat caaagatcac gaaatgcgcg gagtgtcctt
     3901 tcaacaagtg ggatatcacc gtttttttat gatgttgttt atggaattgt gtaccgctga
     3961 cgtcatcctg gaaagcttga tgcatagcat cgtcagcgct tttgcttaca catatcactt
     4021 gttgaatccc agcgtggctc cgggattctg ctttgcttgg ttggagctca tttcgcatcg
     4081 cgtcttcctg ggccgcatcc tggtccaaat tcccgggcaa aagggctggc cactttacgc
     4141 tcaattgctg caggaccttt ttaaatatct tgcgcccttc ttacgcaaca ctgagctcgg
     4201 caaaccagtc cagcttttgt acaaggggac gctacgtgtt ctgctcgttc tgcttcacga
     4261 cttcccggag ttcttgtgcg actatcattt tgggttctgt gacaccatcc cgcccaattg
     4321 cgtccagatg cgtaacatca tcctgtcggc gtttccgcgc aacatgcgct tgcccgatcc
     4381 cttcacgccc aacctaaaag tggacatgct gtcggacagt agtaatgcgc ctaaggtgct
     4441 cagtagctac attatgaaca tccagcccgc gaactttaag aaggatttgg actcgtacct
     4501 caaggcccgg gcgccagtta cttttctatc ggaattacgt ggtcacttgc aggtgacaag
     4561 cgagccagga actcgttaca acatggcgct gatgaatgct ctggtcatgt atgtgggaac
     4621 tcaggcgatt gctttgatca gaaacaaaaa ctttgtgccc aacacctcga acatagcaca
     4681 cagcgcccac atggatatct tccagaatct tgctgttgac ttggatacgg agggtcgcta
     4741 tctgttcttg aatgcgattg cgaaccagtt gcgctatccc aacagccaca cgcactactt
     4801 cagctgtgcg gtgctgcatc tgtttgccga ggccaactcg gaggcaattc aggagcagat
     4861 cactcgcgta cttctggaac gattgatcgt gaaccgcccg catccttggg gactgttgat
     4921 cacttttatt gagctgatca aaaatcccat ctacaagttc tgggaccatg actttgtgca
     4981 ctgtgcgcca gagatcacca agctctttga atcggtggcc cgttcctgtc tggccaagtc
     5041 gaacgtcacc cagcagttga acatgcccgt cgtcgatggc gagggccagg aggtagccac
     5101 catcaactga tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg
     5161 gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa acccgctgat
     5221 cagcctcgac tgtgccttct aaggcctgag ctcgctgatc agcctcgatc gaggatccag
     5281 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     5341 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     5401 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     5461 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagtcga
     5521 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
     5581 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
     5641 gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg
     5701 tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc
     5761 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc
     5821 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc
     5881 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac
     5941 gaccatcagg gacagcttca aggatcgctc gcggctctta ccagccagca aaaggccagg
     6001 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6061 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6121 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6181 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     6241 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6301 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6361 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6421 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     6481 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6541 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6601 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6661 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6721 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6781 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6841 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6901 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6961 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7021 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7081 ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg
     7141 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7201 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7261 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7321 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7381 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta
     7441 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7501 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7561 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7621 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7681 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     7741 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     7801 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgt
//
