LOCUS       sequence-290970-        9603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HseIF4G1-Y619AL624AL625A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9603)
  AUTHORS   Gruner S, Peter D, Weber R, Wohlbold L, Chung MY, Weichenrieder O, 
            Valkov E, Igreja C, Izaurralde E
  TITLE     The Structures of eIF4E-eIF4G Complexes Reveal an Extended Interface
            to Regulate Translation Initiation.
  JOURNAL   Mol Cell. 2016 Nov 3;64(3):467-479. doi: 
            10.1016/j.molcel.2016.09.020. Epub 2016 Oct 20.
  PUBMED    27773676
REFERENCE   2  (bases 1 to 9603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.09.020"; journalName: "Mol Cell"; date: 
            "2016-11-3- 3"; volume: "64"; issue: "3"; pages: "467-479"
COMMENT     Sequence Label: pT7-EGFP-C1-HseIF4G1-Y619AL624AL625A_AD
FEATURES             Location/Qualifiers
     source          1..9603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(710..731)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(971..990)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1318..1339
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6391..6512
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6428..6447)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6482..6501
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6519..6974)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6656..6677)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6868..6887
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7001..7105
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7107..7464
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7107..7298
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7130..7150)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7315..7450
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7499..8293
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7499..8293
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7553..7572)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8163..8182
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8481..8500)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8525..8572
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8901..9489
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9390..9409
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      721 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      781 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      841 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      901 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      961 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1021 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1081 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1141 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1201 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1261 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1321 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1381 gtccggactc agatctcgag ctatgaacaa agctccacag tccacaggcc ccccacccgc
     1441 cccatccccc ggactcccac agccagcgtt tcccccgggg cagacagcgc cggtggtgtt
     1501 cagtacgcca caagcgacac aaatgaacac gccttctcag ccccgccagg gaggattcag
     1561 gtctctgcag cacttctacc ctagccgggc ccagcccccg agcagtgcag cctcccgagt
     1621 gcagagtgca gcccctgccc gccctggccc agctgcccat gtctaccctg ctggatccca
     1681 agtaatgatg atcccttccc agatctccta cccagcctcc cagggggcct actacatccc
     1741 tggacagggg cgttccacat acgttgtccc gacacagcag taccctgtgc agccaggagc
     1801 cccaggcttc tatccaggtg caagccctac agaatttggg acctacgctg gcgcctacta
     1861 tccagcccaa ggggtgcagc agtttcccac tggcgtggcc cccgccccag ttttgatgaa
     1921 ccagccaccc cagattgctc ccaagaggga gcgtaagacg atccgaattc gagatccaaa
     1981 ccaaggagga aaggatatca cagaggagat catgtctggg gcccgcactg cctccacacc
     2041 cacccctccc cagacgggag gcggtctgga gcctcaagct aatggggaga cgccccaggt
     2101 tgctgtcatt gtccggccag atgaccggtc acagggagca atcattgctg accggccagg
     2161 gctgcctggc ccagagcata gcccttcaga atcccagcct tcgtcgcctt ctccgacccc
     2221 atcaccatcc ccagtcttgg aaccggggtc tgagcctaat ctcgcagtcc tctctattcc
     2281 tggggacact atgacaacta tacaaatgtc tgtagaagaa tcaaccccca tctcccgtga
     2341 aactggggag ccatatcgcc tctctccaga acccactcct ctcgccgaac ccatactgga
     2401 agtagaagtg acacttagca aaccggttcc agaatctgag ttttcttcca gtcctctcca
     2461 ggctcccacc cctttggcat ctcacacagt ggaaattcat gagcctaatg gcatggtccc
     2521 atctgaagat ctggaaccag aggtggagtc aagcccagag cttgctcctc ccccagcttg
     2581 cccctccgaa tcccctgtgc ccattgctcc aactgcccaa cctgaggaac tgctcaacgg
     2641 agccccctcg ccaccagctg tggacttaag cccagtcagt gagccagagg agcaggccaa
     2701 ggaggtgaca gcatcagtgg cgccccccac catcccctct gctactccag ctacggctcc
     2761 ttcagctact tccccagctc aggaggagga aatggaagaa gaagaagaag aggaagaagg
     2821 agaagcagga gaagcaggag aagctgagag tgagaaagga ggagaggaac tgctcccccc
     2881 agagagtacc cctattccag ccaacttgtc tcagaatttg gaggcagcag cagccactca
     2941 agtggcagta tctgtgccaa agaggagacg gaaaattaag gagctaaata agaaggaggc
     3001 tgttggagac cttctggatg ccttcaagga ggcgaacccg gcagtaccag aggtggaaaa
     3061 tcagcctcct gcaggcagca atccaggccc agagtctgag ggcagtggtg tgcccccacg
     3121 tcctgaggaa gcagatgaga cctgggactc aaaggaagac aaaattcaca atgctgagaa
     3181 catccagccc ggggaacaga agtatgaata taagtcagat cagtggaagc ctctaaacct
     3241 agaggagaaa aaacgtgcgg accgtgagtt cgcggccggt tttcagttca tctttgccag
     3301 tatgcagaag ccagagggat tgccacatat cagtgacgtg gtgctggaca aggccaataa
     3361 aacaccactg cggccactgg atcccactag actacaaggc ataaattgtg gcccagactt
     3421 cactccatcc tttgccaacc ttggccggac aacccttagc acccgtgggc ccccaagggg
     3481 tgggccaggt ggggagctgc cccgtgggcc ggctggcctg ggaccccggc gctctcagca
     3541 gggaccccga aaagaaccac gcaagatcat tgccacagtg ttaatgaccg aagatataaa
     3601 actgaacaaa gcagagaaag cctggaaacc cagcagcaag cggacggcgg ctgataagga
     3661 tcgaggggaa gaagatgctg atggcagcaa aacccaggac ctattccgca gggtgcgctc
     3721 catcctgaat aaactgacac cccagatgtt ccagcagctg atgaagcaag tgacgcagct
     3781 ggccatcgac accgaggaac gcctcaaagg ggtcattgac ctcatttttg agaaggccat
     3841 ttcagagccc aacttctctg tggcctatgc caacatgtgc cgctgcctca tggcgctgaa
     3901 agtgcccact acggaaaagc caacagtgac tgtgaacttc cgaaagctgt tgttgaatcg
     3961 atgtcagaag gagtttgaga aagacaaaga tgatgatgag gtttttgaga agaagcaaaa
     4021 agagatggat gaagctgcta cggcagagga acgaggacgc ctgaaggaag agctggaaga
     4081 ggctcgggac atagcccggc ggcgctcttt agggaatatc aagtttattg gagagttgtt
     4141 caaactgaag atgttaacag aggcaataat gcatgactgt gtggtcaaac tgcttaagaa
     4201 ccatgatgaa gagtcccttg agtgcctttg tcgtctgctc accaccattg gcaaagacct
     4261 ggactttgaa aaagccaagc cccgaatgga tcagtatttc aaccagatgg aaaaaatcat
     4321 taaagaaaag aagacgtcat cccgcatccg ctttatgctg caggacgtgc tggatctgcg
     4381 agggagcaat tgggtgccac gccgagggga tcagggtccc aagaccattg accagatcca
     4441 taaggaggct gagatggaag aacatcgaga gcacatcaaa gtgcagcagc tcatggccaa
     4501 gggcagtgac aagcgtcggg gcggtcctcc aggccctccc atcagccgtg gacttcccct
     4561 tgtggatgat ggtggctgga acacagttcc catcagcaaa ggtagccgcc ccattgacac
     4621 ctcacgactc accaagatca ccaagcctgg ctccatcgat tctaacaacc agctctttgc
     4681 acctggaggg cgactgagct ggggcaaggg cagcagcgga ggctcaggag ccaagccctc
     4741 agacgcagca tcagaagctg ctcgcccagc tactagtact ttgaatcgct tctcagccct
     4801 tcaacaagcg gtacccacag aaagcacaga taatagacgt gtggtgcaga ggagtagctt
     4861 gagccgagaa cgaggcgaga aagctggaga ccgaggagac cgcctagagc ggagtgaacg
     4921 gggaggggac cgtggggacc ggcttgatcg tgcgcggaca cctgctacca agcggagctt
     4981 cagcaaggaa gtggaggagc ggagtagaga acggccctcc cagcctgagg ggctgcgcaa
     5041 ggcagctagc ctcacggagg atcgggaccg tgggcgggat gccgtgaagc gagaagctgc
     5101 cctaccccca gtgagccccc tgaaggcggc tctctctgag gaggagttag agaagaaatc
     5161 caaggctatc attgaggaat atctccatct caatgacatg aaagaggcag tccagtgcgt
     5221 gcaggagctg gcctcaccct ccttgctctt catctttgta cggcatggtg tcgagtctac
     5281 gctggagcgc agtgccattg ctcgtgagca tatggggcag ctgctgcacc agctgctctg
     5341 tgctgggcat ctgtctactg ctcagtacta ccaagggttg tatgaaatct tggaattggc
     5401 tgaggacatg gaaattgaca tcccccacgt gtggctctac ctagcggaac tggtaacacc
     5461 cattctgcag gaaggtgggg tgcccatggg ggagctgttc agggagatta caaagcctct
     5521 gagaccgttg ggcaaagctg cttccctgtt gctggagatc ctgggcctcc tgtgcaaaag
     5581 catgggtcct aaaaaggtgg ggacgctgtg gcgagaagcc gggcttagct ggaaggaatt
     5641 tctacctgaa ggccaggaca ttggtgcatt cgtcgctgaa cagaaggtgg agtataccct
     5701 gggagaggag tcggaagccc ctggccagag ggcactcccc tccgaggagc tgaacaggca
     5761 gctggagaag ctgctgaagg agggcagcag taaccagcgg gtgttcgact ggatagaggc
     5821 caacctgagt gagcagcaga tagtatccaa cacgttagtt cgagccctca tgacggctgt
     5881 ctgctattct gcaattattt ttgagactcc cctccgagtg gacgttgcag tgctgaaagc
     5941 gcgagcgaag ctgctgcaga aatacctgtg tgacgagcag aaggagctac aggcgctcta
     6001 cgccctccag gcccttgtag tgaccttaga acagcctccc aacctgctgc ggatgttctt
     6061 tgacgcactg tatgacgagg acgtggtgaa ggaggatgcc ttctacagtt gggagagtag
     6121 caaggacccc gctgagcagc agggcaaggg tgtggccctt aaatctgtca cagccttctt
     6181 caagtggctc cgtgaagcag aggaggagtc tgaccacaac tgaaagcttc gaattctgca
     6241 gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat aatcagccat
     6301 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     6361 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     6421 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6481 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt
     6541 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     6601 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     6661 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     6721 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     6781 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     6841 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     6901 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     6961 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     7021 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     7081 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
     7141 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7201 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7261 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7321 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7381 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7441 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
     7501 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     7561 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     7621 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     7681 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     7741 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     7801 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     7861 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     7921 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     7981 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     8041 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     8101 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     8161 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     8221 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     8281 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     8341 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     8401 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     8461 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     8521 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
     8581 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     8641 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     8701 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
     8761 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8821 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8881 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8941 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9001 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     9061 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9121 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9181 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9241 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9301 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9361 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9421 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9481 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9541 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
     9601 cat
//
