LOCUS       sequence-291038-        8431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmRoquin-del725-755.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Sgromo A, Raisch T, Bawankar P, Bhandari D, Chen Y, Kuzuoglu-Ozturk 
            D, Weichenrieder O, Izaurralde E
  TITLE     A CAF40-binding motif facilitates recruitment of the CCR4-NOT 
            complex to mRNAs targeted by Drosophila Roquin.
  JOURNAL   Nat Commun. 2017 Feb 6;8:14307. doi: 10.1038/ncomms14307.
  PUBMED    28165457
REFERENCE   2  (bases 1 to 8431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-02-6"; volume: "8:14307. doi"; pages: " 
            10.1038/ncomms14307"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmRoquin-del725-755_AE
FEATURES             Location/Qualifiers
     source          1..8431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5716..5757
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5767..5784
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5807..5824)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5857..5991
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5881..5900)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5935..5954
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6613..7201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6693..6712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7372..8163,8164..8232))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7995..8014
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8233..8337)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8405..8423
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgatgccga ttcaggctcc ctcatggaca gattttctga actgtcccat
     3361 ctgctgcaat gaattcgcgg ccagccagcg atgtccggtc agtctgggct gcgggcacac
     3421 catctgcaag ctatgcctga cgaccctcta caaccggcag tgtcccttcg atcagaccgt
     3481 gattgtcagt gacatcgaca atttgcccat caaccatgcc ttgctccagt tggtcaagga
     3541 ctcggagctc ctcgagctgg caccaccacc gcccagtgtg cagaagctgg agccggagca
     3601 ccttaagtgt taccaactgg gtcagcggtg catcgaggag ctggctttgc acttgaagtc
     3661 cttcctcaac ctgaacggaa atggcaatct tctaactcga cccatgttac ggaaactggt
     3721 tactctggtc aactgccagc tgatggagga ggagggacgg gtgagagcgc ttcgagcagc
     3781 cagatcccta ggcgaacgca cagtcaccga gctgatcctg cagcaccaaa atccccagca
     3841 gttgagctca aatctgtggg ctgcagtgcg gacaagagga tgccagttcc ttggacccgc
     3901 catgcaggag gaggtgctca aactggtgct gctggccttg gaggagggat cagcgctttc
     3961 gcgcaaagtg ctggtcatgt tcgtcgtgca gcgactagag ccgaagtttc cccaggcctc
     4021 caagacgagt atcggacatg tggtacagct gctctaccgc gccagctgct tcaaggtgtc
     4081 caagcgggag gcggactcgt cgttgatgca gctcaaagag gaattccgta cgtatgacgc
     4141 cctacgccgg gagcacgatg cccaaatcgt tcagatagcc accgaggcgg gtctgcgaat
     4201 tgcaccggaa caatggtctt ccctattgta tggcgacgtg atgcataaaa gccacatgca
     4261 gagcatcatc gataagctgc agacgccaag ctcctttgcg caatcagtcc aggaattggt
     4321 catcgccttg cagcggacca gcgatcctgc gaagctatcc agtttgcatc atcatctgaa
     4381 gtacctggcc aatattgatc cctgtgcgga ggttgccccg tggtcggttt tggctgaggc
     4441 cttggatgca gtgcggcatt cggtcgttgg actggtgaac ttccttcagc accacggagt
     4501 gcgcaaggcg caggacggca tcagtggcgg tggcagtggt gggacgacca atagcaatcc
     4561 gaagtacaag atcagcttat gcagggactt gaatgtgcgg cgggtgtgtc cccgaggttc
     4621 tagctgtacc tttgcccatt cccaggagga ggtagaacgc tatcgtgcac ggaatcgagg
     4681 aaaacatatg aagacaccgt tggcgctgca gggacctcca gcggtaggcg taggagccat
     4741 aaagaaacca ctaggcgagc aggagggacc gcctctaggt aacatgccac ccatgttacc
     4801 tatgtcgcct atgcattata tgggctctcc taggggatat ctggatccca gcttgggact
     4861 ttcgccaggt ggtggtctcc cgccgtcgca tcacagtccc attaccaggc tcatagtgcc
     4921 gagccgctac gattcgcgct ttagcgggtt tggcggtggc acgcccagga ttccttcgcc
     4981 cagagaatac caagccaatc cggtagcgcc aacgcaacgc aatgccaacc caccgaattt
     5041 tagtgtgaac agtaacctgc acaagggcta catgttgccc gccagcggtg gagatgtctt
     5101 ccatctggcc aatccgtggg agcaagcata cctggcacag cagcaacatc ctccacagca
     5161 tccccaacag cagcagccac cgtccagcaa gccgaatccc agccgaccgt tgtcgatttt
     5221 acccgcaaca gctgatacct cattttttga aaagaaaccg ccgaatagtg ttagcataga
     5281 tctggacagg gtcccggagg tcaatgtgga tgcagtgcct ctgttccggc gatccaacaa
     5341 taaccacaat aacaacaata gtaactccca caataacaac aacaacaatc atggctcatc
     5401 actgctgttc tggaacaata cgggtaaaga ttcagccaac tttgtacgat cagactccat
     5461 attggatgac gatgcgtcca cattctatgg cggcttcaag agtgacagaa acgatagctt
     5521 caatgccaat aagcagcaac agccgatgtg gggcaggaaa ccgcaactga tgtcggaaga
     5581 cagtttcgag ggcggcatcg atagtggaat gatgctgcag ctggagaaga atctggtgga
     5641 catcgtcgat cccgatgaca gtgggatcaa ggtggactag gcggccgctc gagtctagag
     5701 ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt
     5761 accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct
     5821 tctaaggcct gagctcgctg atcagcctcg atcgaggatc cagacatgat aagatacatt
     5881 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     5941 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     6001 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     6061 taaaacctct acaaatgtgg tatggctgat tatgatcagt cgaccgatgc ccttgagagc
     6121 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     6181 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt
     6241 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg
     6301 aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga
     6361 gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt
     6421 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
     6481 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
     6541 tcaaggatcg ctcgcggctc ttaccagcca gcaaaaggcc aggaaccgta aaaaggccgc
     6601 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6661 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6721 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6781 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     6841 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6901 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6961 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7021 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     7081 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7141 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7201 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7261 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     7321 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     7381 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     7441 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     7501 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     7561 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     7621 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     7681 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7741 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7801 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7861 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7921 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7981 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8041 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8101 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8161 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     8221 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     8281 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     8341 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     8401 taaaaatagg cgtatcacga ggccctttcg t
//
