LOCUS       sequence-29104-e         655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Lee HH, Kim YS, Kim KH, Heo I, Kim SK, Kim O, Kim HK, Yoon JY, Kim 
            HS, Kim do J, Lee SJ, Yoon HJ, Kim SJ, Lee BG, Song HK, Kim VN, Park
            CM, Suh SW
  TITLE     Structural and functional insights into dom34, a key component of 
            no-go mRNA decay.
  JOURNAL   Mol Cell. 2007 Sep 21. 27(6):938-50.
  PUBMED    17889667
REFERENCE   2  (bases 1 to 655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2007 Sep 21. 27(6):938-50."
COMMENT     Sequence Label: pGEM-7Zf-wild
FEATURES             Location/Qualifiers
     source          1..655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(232..250)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(233..250)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(268..284)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(268..284)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(281..303)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    292..308
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(316..322,323..340,341..346))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    361..382
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(499..516)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcgttcgttg agcgagttct caaaaatgaa caaatgtcga cgatatcccg tggtggtggg
       61 ctgcagccgc caccacgcgc ccctccacgg gatatcggat ccttcaactt ccctgagctc
      121 gaagacgcca aaaacataaa gaaaggctcg aggaattcgg taccccgggt tcgaaatcga
      181 taagcttgga tccggagagc tcccaacgcg ttggatgcat agcttgagta ttctatagtg
      241 tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      301 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      361 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      421 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      481 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      541 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      601 aggaaagaac atgtgagcaa aaggccagca aagggccagg aaccgtaaaa aggcc
//
