LOCUS       sequence-291051-        7927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmRoquin-ILLVtoEEEE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7927)
  AUTHORS   Sgromo A, Raisch T, Bawankar P, Bhandari D, Chen Y, Kuzuoglu-Ozturk 
            D, Weichenrieder O, Izaurralde E
  TITLE     A CAF40-binding motif facilitates recruitment of the CCR4-NOT 
            complex to mRNAs targeted by Drosophila Roquin.
  JOURNAL   Nat Commun. 2017 Feb 6;8:14307. doi: 10.1038/ncomms14307.
  PUBMED    28165457
REFERENCE   2  (bases 1 to 7927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-02-6"; volume: "8:14307. doi"; pages: " 
            10.1038/ncomms14307"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmRoquin-ILLVtoEEEE_AE
FEATURES             Location/Qualifiers
     source          1..7927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5212..5253
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5263..5280
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5303..5320)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5353..5487
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5377..5396)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5431..5450
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6109..6697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6189..6208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6868..7659,7660..7728))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7491..7510
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7729..7833)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7901..7919
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgatgc cgattcaggc tccctcatgg acagattttc tgaactgtcc
     2761 catctgctgc aatgaattcg cggccagcca gcgatgtccg gtcagtctgg gctgcgggca
     2821 caccatctgc aagctatgcc tgacgaccct ctacaaccgg cagtgtccct tcgatcagac
     2881 cgtgattgtc agtgacatcg acaatttgcc catcaaccat gccttgctcc agttggtcaa
     2941 ggactcggag ctcctcgagc tggcaccacc accgcccagt gtgcagaagc tggagccgga
     3001 gcaccttaag tgttaccaac tgggtcagcg gtgcatcgag gagctggctt tgcacttgaa
     3061 gtccttcctc aacctgaacg gaaatggcaa tcttctaact cgacccatgt tacggaaact
     3121 ggttactctg gtcaactgcc agctgatgga ggaggaggga cgggtgagag cgcttcgagc
     3181 agccagatcc ctaggcgaac gcacagtcac cgagctgatc ctgcagcacc aaaatcccca
     3241 gcagttgagc tcaaatctgt gggctgcagt gcggacaaga ggatgccagt tccttggacc
     3301 cgccatgcag gaggaggtgc tcaaactggt gctgctggcc ttggaggagg gatcagcgct
     3361 ttcgcgcaaa gtgctggtca tgttcgtcgt gcagcgacta gagccgaagt ttccccaggc
     3421 ctccaagacg agtatcggac atgtggtaca gctgctctac cgcgccagct gcttcaaggt
     3481 gtccaagcgg gaggcggact cgtcgttgat gcagctcaaa gaggaattcc gtacgtatga
     3541 cgccctacgc cgggagcacg atgcccaaat cgttcagata gccaccgagg cgggtctgcg
     3601 aattgcaccg gaacaatggt cttccctatt gtatggcgac gtgatgcata aaagccacat
     3661 gcagagcatc atcgataagc tgcagacgcc aagctccttt gcgcaatcag tccaggaatt
     3721 ggtcatcgcc ttgcagcgga ccagcgatcc tgcgaagcta tccagtttgc atcatcatct
     3781 gaagtacctg gccaatattg atccctgtgc ggaggttgcc ccgtggtcgg ttttggctga
     3841 ggccttggat gcagtgcggc attcggtcgt tggactggtg aacttccttc agcaccacgg
     3901 agtgcgcaag gcgcaggacg gcatcagtgg cggtggcagt ggtgggacga ccaatagcaa
     3961 tccgaagtac aagatcagct tatgcaggga cttgaatgtg cggcgggtgt gtccccgagg
     4021 ttctagctgt acctttgccc attcccagga ggaggtagaa cgctatcgtg cacggaatcg
     4081 aggaaaacat atgaagacac cgttggcgct gcagggacct ccagcggtag gcgtaggagc
     4141 cataaagaaa ccactaggcg agcaggaggg accgcctcta ggtaacatgc cacccatgtt
     4201 acctatgtcg cctatgcatt atatgggctc tcctagggga tatctggatc ccagcttggg
     4261 actttcgcca ggtggtggtc tcccgccgtc gcatcacagt cccattacca ggctcatagt
     4321 gccgagccgc tacgattcgc gctttagcgg gtttggcggt ggcacgccca ggattccttc
     4381 gcccagagaa taccaagcca atccggtagc gccaacgcaa cgcaatgcca acccaccgaa
     4441 ttttagtgtg aacagtaacc tgcacaaggg ctacatgttg cccgccagcg gtggagatgt
     4501 cttccatctg gccaatccgt gggagcaagc atacctggca cagcagcaac atcctccaca
     4561 gcatccccaa cagcagcagc caccgtccag caagccgaat cccagccgac cgttgtcgat
     4621 tttacccgca acagctgata cctcattttt tgaaaagaaa ccgccgaata gtgttagcat
     4681 agatctggac agggtcccgg aggtcaatgt ggatgcagtg cctctgttcc ggcgatccaa
     4741 caataaccac aataacaaca atagtaactc ccacaataac aacaacaaca atcatggctc
     4801 atcactgctg ttctggaaca atacgggtaa agattcagcc aactttgtac gatcagactc
     4861 catattggat gacgatgcgt ccacattcga tgtgcccact ggctcctcca tgctgagtat
     4921 ctacggcccg atttgcccca agagcagtac gaccggcaac tggaacaact ttgatctggg
     4981 atatggcggc ttcaagagtg acagaaacga tagcttcaat gccaataagc agcaacagcc
     5041 gatgtggggc aggaaaccgc aactgatgtc ggaagacagt ttcgagggcg gcgaagatag
     5101 tggaatgatg ctgcaggaag agaagaatga agtggacatc gaagatcccg atgacagtgg
     5161 gatcaaggtg gactaggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct
     5221 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat
     5281 tgagtttaaa cccgctgatc agcctcgact gtgccttcta aggcctgagc tcgctgatca
     5341 gcctcgatcg aggatccaga catgataaga tacattgatg agtttggaca aaccacaact
     5401 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     5461 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     5521 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     5581 gctgattatg atcagtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
     5641 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     5701 cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag
     5761 cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc
     5821 cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat
     5881 ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt
     5941 ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct
     6001 gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg cggctcttac
     6061 cagccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6121 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6181 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6241 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6301 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6361 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6421 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6481 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6541 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6601 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6661 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6721 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6781 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6841 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6901 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6961 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7021 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7081 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7141 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc
     7201 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7261 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7321 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7381 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7441 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc
     7501 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7561 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7621 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7681 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7741 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     7801 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     7861 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     7921 ctttcgt
//
