LOCUS       sequence-291057-        8258 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of DmRoquin-del790-812.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8258)
  AUTHORS   Sgromo A, Raisch T, Bawankar P, Bhandari D, Chen Y, Kuzuoglu-Ozturk 
            D, Weichenrieder O, Izaurralde E
  TITLE     A CAF40-binding motif facilitates recruitment of the CCR4-NOT 
            complex to mRNAs targeted by Drosophila Roquin.
  JOURNAL   Nat Commun. 2017 Feb 6;8:14307. doi: 10.1038/ncomms14307.
  PUBMED    28165457
REFERENCE   2  (bases 1 to 8258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-02-6"; volume: "8:14307. doi"; pages: " 
            10.1038/ncomms14307"
COMMENT     Sequence Label: pcDNA3.1-MS2-HA-DmRoquin-del790-812_AE
FEATURES             Location/Qualifiers
     source          1..8258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      895..904
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1333..1359
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3814..3832)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3815..3832)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3852..3869)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3858..4082
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4128..4556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4215..4234)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4425..4446
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4565..4585)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4570..4899
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4750..4885
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4812..4831
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4966..5760
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5020..5039)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5630..5649
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5934..6055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5971..5990)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6025..6044
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6104..6120)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6104..6120)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6117..6139)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6128..6144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6152..6158,6159..6176,6177..6182))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6197..6218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6335..6352)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6506..7091)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6586..6605)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7262..8053,8054..8122))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7885..7904
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8123..8227)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgccacc
      901 atggcttcta actttactca gttcgttctc gtcgacaatg gcggaactgg cgacgtgact
      961 gtcgccccaa gcaacttcgc taacggggtc gctgaatgga tcagctctaa ctcgcgatca
     1021 caggcttaca aagtaacctg tagcgttcgt cagagctctg cgcagaatcg caaatacacc
     1081 atcaaagtcg aggtgcctaa agtggcaacc cagactgttg gtggtgaaga gcttcctgta
     1141 gccggatgga gatcttactt aaatatggaa ctaaccattc caattttcgc cacgaattcc
     1201 gactgcgagc ttattgttaa ggcaatgcaa ggtctcctaa aagatggaaa cccgattccc
     1261 tcggccatcg cggccaactc cggcatctac ggaggtggag gtggatctgg tggatctcct
     1321 ccacatatgg cttacccata cgatgttcca gattacgctc ctccaaagct tggatccgaa
     1381 ttcccgggtc gactcgagtc tagagcggcc gccatgccga ttcaggctcc ctcatggaca
     1441 gattttctga actgtcccat ctgctgcaat gaattcgcgg ccagccagcg atgtccggtc
     1501 agtctgggct gcgggcacac catctgcaag ctatgcctga cgaccctcta caaccggcag
     1561 tgtcccttcg atcagaccgt gattgtcagt gacatcgaca atttgcccat caaccatgcc
     1621 ttgctccagt tggtcaagga ctcggagctc ctcgagctgg caccaccacc gcccagtgtg
     1681 cagaagctgg agccggagca ccttaagtgt taccaactgg gtcagcggtg catcgaggag
     1741 ctggctttgc acttgaagtc cttcctcaac ctgaacggaa atggcaatct tctaactcga
     1801 cccatgttac ggaaactggt tactctggtc aactgccagc tgatggagga ggagggacgg
     1861 gtgagagcgc ttcgagcagc cagatcccta ggcgaacgca cagtcaccga gctgatcctg
     1921 cagcaccaaa atccccagca gttgagctca aatctgtggg ctgcagtgcg gacaagagga
     1981 tgccagttcc ttggacccgc catgcaggag gaggtgctca aactggtgct gctggccttg
     2041 gaggagggat cagcgctttc gcgcaaagtg ctggtcatgt tcgtcgtgca gcgactagag
     2101 ccgaagtttc cccaggcctc caagacgagt atcggacatg tggtacagct gctctaccgc
     2161 gccagctgct tcaaggtgtc caagcgggag gcggactcgt cgttgatgca gctcaaagag
     2221 gaattccgta cgtatgacgc cctacgccgg gagcacgatg cccaaatcgt tcagatagcc
     2281 accgaggcgg gtctgcgaat tgcaccggaa caatggtctt ccctattgta tggcgacgtg
     2341 atgcataaaa gccacatgca gagcatcatc gataagctgc agacgccaag ctcctttgcg
     2401 caatcagtcc aggaattggt catcgccttg cagcggacca gcgatcctgc gaagctatcc
     2461 agtttgcatc atcatctgaa gtacctggcc aatattgatc cctgtgcgga ggttgccccg
     2521 tggtcggttt tggctgaggc cttggatgca gtgcggcatt cggtcgttgg actggtgaac
     2581 ttccttcagc accacggagt gcgcaaggcg caggacggca tcagtggcgg tggcagtggt
     2641 gggacgacca atagcaatcc gaagtacaag atcagcttat gcagggactt gaatgtgcgg
     2701 cgggtgtgtc cccgaggttc tagctgtacc tttgcccatt cccaggagga ggtagaacgc
     2761 tatcgtgcac ggaatcgagg aaaacatatg aagacaccgt tggcgctgca gggacctcca
     2821 gcggtaggcg taggagccat aaagaaacca ctaggcgagc aggagggacc gcctctaggt
     2881 aacatgccac ccatgttacc tatgtcgcct atgcattata tgggctctcc taggggatat
     2941 ctggatccca gcttgggact ttcgccaggt ggtggtctcc cgccgtcgca tcacagtccc
     3001 attaccaggc tcatagtgcc gagccgctac gattcgcgct ttagcgggtt tggcggtggc
     3061 acgcccagga ttccttcgcc cagagaatac caagccaatc cggtagcgcc aacgcaacgc
     3121 aatgccaacc caccgaattt tagtgtgaac agtaacctgc acaagggcta catgttgccc
     3181 gccagcggtg gagatgtctt ccatctggcc aatccgtggg agcaagcata cctggcacag
     3241 cagcaacatc ctccacagca tccccaacag cagcagccac cgtccagcaa gccgaatccc
     3301 agccgaccgt tgtcgatttt acccgcaaca gctgatacct cattttttga aaagaaaccg
     3361 ccgaatagtg ttagcataga tctggacagg gtcccggagg tcaatgtgga tgcagtgcct
     3421 ctgttccggc gatccaacaa taaccacaat aacaacaata gtaactccca caataacaac
     3481 aacaacaatc atggctcatc actgctgttc tggaacaata cgggtaaaga ttcagccaac
     3541 tttgtacgat cagactccat attggatgac gatgcgtcca cattcgatgt gcccactggc
     3601 tcctccatgc tgagtatcta cggcccgatt tgccccaaga gcagtacgac cggcaactgg
     3661 aacaactttg atctgggata tggcggcttc aagagtgaca gaaacgatag cttcaatgcc
     3721 aataagcagc aacagccgat gtggggcagg aaaccgcaac tgatgtcgga agacagtttc
     3781 gacagtggga tcaaggtgga ctaggggccc tattctatag tgtcacctaa atgctagagc
     3841 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3901 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     3961 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     4021 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     4081 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     4141 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4201 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4261 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4321 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4381 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4441 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4501 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     4561 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     4621 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     4681 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     4741 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4801 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     4861 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     4921 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     4981 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     5041 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     5101 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     5161 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     5221 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     5281 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     5341 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     5401 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     5461 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     5521 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     5581 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     5641 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     5701 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     5761 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     5821 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     5881 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     5941 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6001 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6061 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     6121 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6181 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6241 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6301 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6361 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6421 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6481 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6541 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6601 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6661 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6721 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6781 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6841 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6901 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6961 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7021 aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7081 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7141 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7201 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7261 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7321 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7381 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7441 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7501 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7561 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7621 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7681 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7741 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7801 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7861 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7921 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7981 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8041 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8101 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8161 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8221 ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
