LOCUS       sequence-29114-e         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Jang JY, Yoon HJ, Yoon JY, Kim HS, Lee SJ, Kim KH, Kim do J, Jang S,
            Han BG, Lee BI, Suh SW
  TITLE     Crystal structure of the TNF-alpha-Inducing protein (Tipalpha) from 
            Helicobacter pylori: Insights into Its DNA-binding activity.
  JOURNAL   J Mol Biol. 2009 Sep 11. 392(1):191-7.
  PUBMED    19596016
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2009 Sep 11. 392(1):191-7."
COMMENT     Sequence Label: Tip alpha (21-192)
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..39
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             58..75
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             85..102
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tcccctctag aataattttg tttaacttta agaaggagat ataccatggg cagcagccat
       61 catcatcatc atcacagcag cggcctggtg ccgcgcggca gccatatgct gcaggcttgc
      121 acttgcccaa acacttcaca aaggaattct ttcttgcaag atgtgcctta ttggatgttg
      181 caaaatcgca gtgagtatat cacgcaaggg gtggatagct cgcacattgt agatggtaag
      241 aaaactgaag agatagaaaa aatcgctacc aaaagagcga caataagagt ggcacaaaat
      301 attgtgcata aacttaaaga agcttacctt tccaaaacca atcgcatcaa gcaaaagatc
      361 actaatgaaa tgtttatcca aatgacacag cccatttatg acagcttgat gaatgtggat
      421 cgtttaggga tttatatcaa tcctaacaat gaggaagtgt ttgcgttagt gcgcgcgcgt
      481 ggttttgata aggacgcttt gagcgaaggg ttgcataaaa tgtccttaga caatcaagcg
      541 gtgagtatcc ttgtggctaa agtggaagaa atctttaaag attctgtcaa ttacggagat
      601 gttaaagtcc ctatagccat gtaactcgag ctnccacccc ccccaccacc actgaga
//
