LOCUS       sequence-29115-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Jang JY, Yoon HJ, Yoon JY, Kim HS, Lee SJ, Kim KH, Kim do J, Jang S,
            Han BG, Lee BI, Suh SW
  TITLE     Crystal structure of the TNF-alpha-Inducing protein (Tipalpha) from 
            Helicobacter pylori: Insights into Its DNA-binding activity.
  JOURNAL   J Mol Biol. 2009 Sep 11. 392(1):191-7.
  PUBMED    19596016
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2009 Sep 11. 392(1):191-7."
COMMENT     Sequence Label: Tip alpha (28-192)
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..39
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             58..75
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             85..102
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             613..630
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcccctctag aataattttg tttaacttta agaaggagat ataccatggg cagcagccat
       61 catcatcatc atcacagcag cggcctggtg ccgcgcggca gccatatgcc aaacacttca
      121 caaaggaatt cttttttaca agatgtgcct tattggatgt tgcaaaatcg cagccagtat
      181 ctcacgcaag gggtggatag ctcgcacatt gtagatggta aggcaactga agagatagag
      241 aaaatcgcta ccaaaagagc gacaataaga gtggcgcaaa atatcgtgca taaacttaaa
      301 gaagcttatc tttctaaatc caaccgcatc aagcaaaaga tcactaatga aatgtttatc
      361 caaatgacaa agcccatttt cgacagcttg atgaatgtgg atcgtttagg gatttatatc
      421 aatcctaaca atgaagaagt gtttgcgttg gtgcgcgcgc gttcctttga taaggacgct
      481 ttgagcgaag ggttgcataa aatgtcttta gacgatcaag cggtgagtat ccttgtttct
      541 aaagtggaag aaatctttaa agattctatc aattacggag atgttaaagt ccctatagcc
      601 atgtaactcg agcaccacca ccaccaccac tgaganccgg ctgctaacaa agcccaaaan
      661 ggaagctgag ttgggctgct gccaaccgcc tgagcaatnc naagcataaa ccccttgggg
      721 gcctctaaac ggtgtctttg aggggttttt tgctgaaagg aggatctata tccgggattg
      781 gccgaatggg gacgcgccct gtagcggcgc atttaggcgc ggccgggtgg tggtgggtta
      841 cggcnncagg cgngaccgcc taccacttgc cca
//
