LOCUS       sequence-29116-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Jang JY, Yoon HJ, Yoon JY, Kim HS, Lee SJ, Kim KH, Kim do J, Jang S,
            Han BG, Lee BI, Suh SW
  TITLE     Crystal structure of the TNF-alpha-Inducing protein (Tipalpha) from 
            Helicobacter pylori: Insights into Its DNA-binding activity.
  JOURNAL   J Mol Biol. 2009 Sep 11. 392(1):191-7.
  PUBMED    19596016
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2009 Sep 11. 392(1):191-7."
COMMENT     Sequence Label: Tip alpha K102A/K104A (21-192)
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             59..76
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             86..103
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(711..729)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ttcccctcta gantaatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca
       61 tcatcatcat catcacagca gcggcctggt gccgcgcggc agccatatgc tgcaggcttg
      121 cacttgccca aacacttcac aaaggaattc tttcttgcaa gatgtgcctt attggatgtt
      181 gcaaaatcgc agtgagtata tcacgcaagg ggtggatagc tcgcacattg tagatggtaa
      241 gaaaactgaa gagatagaaa aaatcgctac caaaagagcg acaataagag tggcacaaaa
      301 tattgtgcat aaacttaaag aagcttacct ttccaaaacc aatcgcatcg cgcaagcgat
      361 cactaatgaa atgtttatcc aaatgacaca gcccatttat gacagcttga tgaatgtgga
      421 tcgtttaggg atttatatca atcctaacaa tgaggaagtg tttgcgttag tgcgcgcgcg
      481 tggttttgat aaggacgctt tgagcgaagg gttgcataaa atgtccttag acaatcaagc
      541 ggtgagtatc cttgtggcta aagtggaaga aatctttaaa gattctgtca attacggaga
      601 tgttaaagtc cctatagcca tgtaacctcg agccacccac cacccaccca ccactgagat
      661 cccggcnnnn taacaaagcc cgaaaggaaa gctgagttgg ctgctgccaa ccgctgagca
      721 ataactagca taaccctctt ggggcctcta aacgggtgcn tcgtggaggt ggtttttttg
      781 ctgaaaggag gact
//
