LOCUS       sequence-29117-e         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Tang JB, Goellner EM, Wang XH, Trivedi RN, St Croix CM, Jelezcova E,
            Svilar D, Brown AR, Sobol RW
  TITLE     Bioenergetic metabolites regulate base excision repair-dependent 
            cell death in response to DNA damage.
  JOURNAL   Mol Cancer Res. 2010 Jan;8(1):67-79. doi: 
            10.1158/1541-7786.MCR-09-0411. Epub 2010 Jan 12.
  PUBMED    20068071
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/1541-7786.MCR-09-0411"; journalName: "Mol Cancer Res"; 
            date: "2010-01"; volume: "8"; issue: "1"; pages: "67-79"
COMMENT     Sequence Label: pSIF-H1-hpolb-copGFP
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    318..439
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(355..374)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     409..428
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      479..614
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(647..663)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(647..663)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(660..682)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    671..687
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(695..701,702..719,720..725))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 cttataagtt ctgtatgaga ccacttggat ccgctggcag acgcaaccta atgggacttc
       61 ctgtcagatc ctattgggtt gtgtctgcca gtttttgaat tctagcgtcc ataagcattc
      121 tttctttcga tagcttccag tgctttgtga aacttcgagg agtctctttg ttgaggactt
      181 ttgagttctc ccttgaggct cccacagata caataaatat ttgagattga accctgtcga
      241 gtatctgtgt aatctttttt acctgtgagg tctcggaatc cgggccgaga acttcgcagc
      301 gagctcattg taccgcgaac ttgtttattg cagcttataa tggttacaaa taaagcaata
      361 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
      421 aactcatcaa tgtatcttat catgtctggn tctagctatc ccgcccctaa ctccgcccat
      481 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
      541 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
      601 cttttttgga ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc
      661 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      721 gtaaagcctg gggtg
//
