LOCUS       sequence-291244-       11959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsHelz.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11959)
  AUTHORS   Hanet A, Rasch F, Weber R, Ruscica V, Fauser M, Raisch T, 
            Kuzuoglu-Ozturk D, Chang CT, Bhandari D, Igreja C, Wohlbold L
  TITLE     HELZ directly interacts with CCR4-NOT and causes decay of bound 
            mRNAs.
  JOURNAL   Life Sci Alliance. 2019 Sep 30;2(5). pii: 2/5/e201900405. doi: 
            10.26508/lsa.201900405. Print 2019 Oct.
  PUBMED    31570513
REFERENCE   2  (bases 1 to 11959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2019-09-30"; volume: "2(5). pii: 2/5/e201900405. 
            doi"; pages: " 10.26508/lsa.201900405. Print 2019 Oct"
COMMENT     Sequence Label: pLIB-2STREP-HsHelz-GSSG-MBP_AG
FEATURES             Location/Qualifiers
     source          1..11959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(4048..4081)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4129..4220
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4145..4162
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4313..4336
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4346..4369
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             10220..11314
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="TEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEK
                     FPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIA
                     YPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAAD
                     GGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETA
                     MTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLEN
                     YLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWY
                     AVRTAVINAASGRQTVDEALKDAQT"
     polyA_signal    11468..11602
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11505..11524)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11559..11578
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  11712..11877
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cagctttgtt tgtactatca acaggttgaa ctgctgatca
     4021 acagatcctc tacgcggccg cggtaccata acttcgtata gcatacatta tacgaagtta
     4081 tctggtttta ctagtatcga ttcgcgacct actccggaat attaatagat catggagata
     4141 attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg
     4201 taataaaaaa acctataaat attccggatt attcataccg tcccaccatc gggcgcggat
     4261 cccggtccga agcgcgcgga attcaaaggc ctacgtcgac atgggcagcg gctggagcca
     4321 cccccagttc gagaagggca gcggctggag ccacccccag ttcgagaagg aagacagaag
     4381 agctgaaaag tcatgtgaac aagcatgtga atcacttaag aggcaggact atgaaatggc
     4441 cctcaagcac tgcacagagg cccttctttc tcttggccag tactccatgg cagacttcac
     4501 agggccttgt ccattggaaa tagaacgcat caaaatcgag agtcttctct acagaattgc
     4561 ctcatttttg caactgaaaa attatgtgca agctgatgaa gattgtagac atgtgctggg
     4621 agaaggactg gccaagggag aagatgcctt tcgggcagtg ctttgctgca tgcagctgaa
     4681 agggaagctc caacctgtat ccaccattct tgccaagtca ctcacaggag agtccctgaa
     4741 tgggatggta acaaaggatt tgacaagact aaaaacactt ctctcagaaa cagagacagc
     4801 aactagtaac gccctctctg gatatcacgt ggaagactta gatgaggggt cttgtaatgg
     4861 ttggcatttc cgcccaccac ctaggggaat cacaagcagc gaggaatata ctttgtgtaa
     4921 aagattttta gaacaaggaa tctgtaggta tggtgcccag tgtacttcag cacattccca
     4981 ggaagaacta gcagaatggc agaaaagata tgcttcacgg ctgataaaat tgaaacagca
     5041 aaatgagaat aaacagctct caggcagtta catggaaacc ttgatagaaa agtggatgaa
     5101 ttcattgtct cctgagaaag tgcttagtga atgtatagaa ggagtaaagg tagagcacaa
     5161 tcctgacctg tcagttactg tcagcaccaa aaaatcccac cagacatgga cctttgctct
     5221 cacttgtaag cctgcaagaa tgctgtatcg tgtagcattg ctttatgatg ctcatcgtcc
     5281 tcattttagt atcattgcaa tatctgccgg agatagtact acccaggtat cacaagaagt
     5341 cccagaaaac tgtcaagaat ggataggagg aaagatggcc caaaatggat tagatcatta
     5401 cgtgtataaa gtcgggatag catttaacac agaaatattt ggaacttttc gccaaaccat
     5461 agttttcgac tttggattgg aaccagtact catgcaaaga gtaatgattg atgcagcttc
     5521 tacagaagat ctcgaatacc tgatgcatgc aaaacagcag ctagtaacca cagctaaacg
     5581 ttgggattct tcctctaaga ctattataga ttttgaacct aatgaaacta ctgatttgga
     5641 gaagagcctt cttatcagat accaaattcc cctctctgct gaccagctat ttactcagtc
     5701 cgttttagac aaatcattga ccaagagcaa ctatcagtca cggttacatg accttcttta
     5761 tattgaggag atagcccagt ataaagaaat cagcaagttc aaccttaaag tgcaattgca
     5821 gattctggca agcttcatgc tcactggtgt ttctggaggt gcaaagtatg ctcagaatgg
     5881 acaacttttt ggtcgcttta agcttactga aacactttct gaagatactt tggctggacg
     5941 actggtgatg accaaagtca atgctgttta tttattacca gtccctaaac agaagttagt
     6001 acagacccag ggaaccaaag agaaggttta tgaagctact attgaagaaa aaacaaagga
     6061 atatatattt ttaaggctat ctagggaatg ctgtgaagaa cttaatcttc ggcctgactg
     6121 tgacacacag gttgaacttc agtttcaatt aaatcgatta cccctctgtg aaatgcacta
     6181 tgcactagac aggatcaagg acaatggggt tttgtttcca gacatcagta tgactcccac
     6241 cataccatgg agtcctaaca gacaatggga tgaacagttg gatcctcgac taaatgcaaa
     6301 acagaaagag gctgttctgg ccattaccac tccacttgca atccagctgc cgcctgtgct
     6361 tatcatcgga ccctatggga caggcaaaac gttcactcta gctcaggctg tcaaacatat
     6421 tctgcagcaa caggagacta ggattctcat ttgcacccat tctaatagtg ctgctgatct
     6481 ctacataaag gattatttac atccatatgt agaagcaggc aatccccagg caagacctct
     6541 cagggtatat ttcagaaatc gctgggtaaa gactgtccac ccagttgtgc atcagtactg
     6601 tttgatctca agcgcacatt ccacctttca gatgccccag aaagaagata ttcttaaaca
     6661 tcgagtggtg gttgttacct tgaatacttc ccagtacctc tgtcagttgg accttgaacc
     6721 tgggtttttt acacacattc tattagatga agctgcccag gccatggagt gtgaaaccat
     6781 tatgcctcta gcattagcaa ctcaaaacac tcggattgtc ttggctggtg atcacatgca
     6841 gctcagtcct tttgtttaca gcgagtttgc cagggagaga aaccttcacg tttcattact
     6901 tgaccgactc tatgagcatt accctgctga gttcccatgt aggattctcc tgtgtgagaa
     6961 ctaccgctcc catgaagcta tcatcaatta tacctctgag cttttctatg agggcaaact
     7021 gatggccagt gggaagcagc cagcacacaa agatttctac ccactaactt tctttacagc
     7081 acgaggagaa gatgtacaag aaaaaaatag cacagctttt tataataatg cagaggtgtt
     7141 tgaagtggtg gaacgtgtag aagagttaag aaggaagtgg ccagtagcgt gggggaagtt
     7201 agatgatggc agtattggtg tggtgactcc atatgctgat caagtgttta gaatacgtgc
     7261 tgaacttcga aaaaagagat tatctgatgt taatgtagaa agggtgctaa atgttcaagg
     7321 aaagcaattc agagttttgt ttcttagcac agtacgtaca agacatactt gtaaacataa
     7381 acagacacca attaaaaaga aagagcaact tctggaagat tccacagagg acttagatta
     7441 tggtttttta tctaactaca agcttctcaa tactgccatc acaagagcac aatccctggt
     7501 tgctgtggtg ggtgatccca ttgctctgtg ctctattgga agatgcagga aattttggga
     7561 acggtttatt gccctgtgtc atgaaaacag tagcctacat ggaatcactt ttgaacagat
     7621 caaagcccag ttagaggctt tagaactaaa gaagacatat gtgttgaatc cgctggcacc
     7681 tgaatttatc ccccgggctc taagactgca gcattcagga agtaccaaca aacagcagca
     7741 atcaccaccc aaggggaaaa gtcttcatca tacccagaat gatcacttcc agaatgatgg
     7801 aattgttcag cccaatcctt ctgtacttat tggcaatcct attagagcat atactcctcc
     7861 accccctctt ggacctcacc caaatttggg aaaatctcca agccctgttc aaagaataga
     7921 tcctcacact gggacaagta ttctttatgt acctgctgtc tatggaggga atgtagttat
     7981 gtcggtgcct ttacctgtac catggacagg ataccagggt aggtttgcag ttgatcctcg
     8041 aattattaca catcaggcag caatggccta taacatgaac ctattacaga cacatggacg
     8101 aggatctcct attccttatg gccttggaca tcacccacct gtcaccatag gccagccaca
     8161 aaatcagcat caggagaagg atcaacatga gcaaaatcga aatggtaaaa gtgatacaaa
     8221 taattccgga cctgaaatta ataagattcg aacaccagag aaaaagccaa cagaaccaaa
     8281 acaggttgat ttggaatcaa atccacagaa cagaagtcct gaatcacgtc ctagtgttgt
     8341 ttatcccagt accaaatttc ctcgcaaaga taatctcaac ccaagacaca taaatcttcc
     8401 ccttcctgct ccccacgcac agtatgcaat ccctaatcgc cactttcatc cccttcccca
     8461 gctaccaaga ccaccctttc caattccaca gcagcacacc ttgttaaatc agcagcagaa
     8521 taatttgcct gaacaaccaa atcagatacc acctcagcca aatcaggtag tccagcagca
     8581 aagtcagttg aatcagcagc ctcagcagcc acctcctcag ctttctcctg catatcaggc
     8641 gggacccaac aatgcttttt ttaatagtgc agttgctcat cggccacagt ctcctcctgc
     8701 agaagctgta attccggagc agcagccccc tcccatgctg caagaaggcc acagtcctct
     8761 gagagccatt gcacaacccg gccccattct tccttcacat ctgaatagct tcattgatga
     8821 gaacccctcg ggattaccta taggggaggc tttagatcgt atacatggga gtgtcgctct
     8881 ggaaacatta aggcagcagc aggcacggtt ccagcagtgg agcgagcatc atgcctttct
     8941 cagtcagggc agcgttccat acccacacca tcaccatcct cacctccagc atcttcctca
     9001 gccgcccctg ggattacatc agccgccagt gagggcagac tggaagctca ccagcagtgc
     9061 cgaagatgaa gtggagacca catactcaag gtttcaagac ttaatcagag aactgtctca
     9121 tcgtgatcaa agtgaaacac gggaactagc tgaaatgcca ccacctcaat caagactttt
     9181 gcaatataga caagtacaga gtagaagccc accagcagtc ccatctcccc catccagtac
     9241 agaccacagt agccactttt ctaactttaa tgataacagc agagacattg aagtagccag
     9301 caacccagca tttccacagc gcctcccacc ccagatattc aactcacctt tctcgttgcc
     9361 atctgaacac cttgcccctc ctcccttgaa atacctggca cctgatggag catggacttt
     9421 tgctaacttg caacagaatc acctaatggg gccaggtttt ccctatggcc tacctccatt
     9481 gcctcacagg ccaccgcaga acccttttgt acaaatacag aatcatcaac atgctattgg
     9541 tcaagagcca tttcacccat tgtcatctcg aacagtatct tcttcttcgc tccctagctt
     9601 agaagagtat gagcccagag gacctggtcg gcccttgtac caaagaagaa tctcatctag
     9661 ctcagttcaa ccttgttctg aagaagtaag cactcctcaa gacagtctgg ctcagtgtaa
     9721 agagcttcag gaccacagta accaatcttc tttcaacttt tcatccccgg agtcctgggt
     9781 aaacaccacc tcatctactc cttatcagaa cattccgtgc aatggatcca gcaggacagc
     9841 tcagcccaga gagttgatag cgccacccaa gactgtcaaa ccccctgagg atcaactgaa
     9901 gtcggagaac ctcgaggtgt ccagttcctt caactacagt gtgctgcagc atcttggcca
     9961 gtttccaccc cttatgccta acaagcagat cgcggagtcg gccaatagca gtagccccca
    10021 gagctctgcg gggggcaagc ccgccatgtc ctatgccagc gctctgcggg cccctccaaa
    10081 gcccaggccc cctcctgagc aggccaagaa gagtagcgac cctctgtctc tcttccagga
    10141 actgagccta gggagctcat ctggcagcaa tggcttttac tcatatttta aagggagctc
    10201 aggcgggagc tcaggcgaaa ctgaagaagg taaactggta atctggatta acggcgataa
    10261 aggctataac ggtctcgctg aagtcggtaa gaaattcgag aaagataccg gaattaaagt
    10321 caccgttgag catccggata aactggaaga gaaattccca caggttgcgg caactggcga
    10381 tggccctgac attatcttct gggcacacga ccgctttggt ggctacgctc aatctggcct
    10441 gttggctgaa atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt ttacctggga
    10501 tgccgtacgt tacaacggca agctgattgc ttacccgatc gctgttgaag cgttatcgct
    10561 gatttataac aaagacctgc tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct
    10621 ggataaagaa ctgaaagcga aaggtaagag cgccctgatg ttcaacctgc aagaaccgta
    10681 cttcacctgg ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa
    10741 gtacgacatt aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct
    10801 ggttgacctg attaaaaaca aacacatgaa tgcagacacc gattactcca tcgcagaagc
    10861 tgcctttaat aaaggcgaaa cagcgatgac catcaacggc ccgtgggcat ggtccaacat
    10921 cgacaccagc aaagtgaatt atggtgtaac ggtactgccg accttcaagg gtcaaccatc
    10981 caaaccgttc gttggcgtgc tgagcgcagg tattaacgcc gccagtccga acaaagagct
    11041 ggcaaaagag ttcctcgaaa actatctgct gactgatgaa ggtctggaag cggttaataa
    11101 agacaaaccg ctgggtgccg tagcgctgaa gtcttacgag gaagagttgg cgaaagatcc
    11161 acgtattgcc gccacgatgg aaaacgccca gaaaggtgaa atcatgccga acatcccgca
    11221 gatgtccgct ttctggtatg ccgtgcgtac tgcggtgatc aacgccgcca gcggtcgtca
    11281 gactgtcgat gaagccctta aagacgcgca gacttaatct agagcctgca gtctcgacaa
    11341 gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag aggttttact
    11401 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    11461 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    11521 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    11581 tgtatcttat catgtctgga tctgatcact gcttgagcct agaagatccg gctgctaaca
    11641 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta tcataacccc
    11701 taggagatcc gaaccagata agtgaaatct agttccaaac tattttgtca tttttaattt
    11761 tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata
    11821 atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc gcccacagcg
    11881 gggcattttt cttcctgtta tgtttttaat caaacatcct gccaactcca tgtgacaaac
    11941 cgtcatcttc ggctacttt
//
