LOCUS       sequence-291245-        9991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsHelz.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Hanet A, Rasch F, Weber R, Ruscica V, Fauser M, Raisch T, 
            Kuzuoglu-Ozturk D, Chang CT, Bhandari D, Igreja C, Wohlbold L
  TITLE     HELZ directly interacts with CCR4-NOT and causes decay of bound 
            mRNAs.
  JOURNAL   Life Sci Alliance. 2019 Sep 30;2(5). pii: 2/5/e201900405. doi: 
            10.26508/lsa.201900405. Print 2019 Oct.
  PUBMED    31570513
REFERENCE   2  (bases 1 to 9991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2019-09-30"; volume: "2(5). pii: 2/5/e201900405. 
            doi"; pages: " 10.26508/lsa.201900405. Print 2019 Oct"
COMMENT     Sequence Label: pLNHA-C1-HsHelz_AG
FEATURES             Location/Qualifiers
     source          1..9991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             665..730
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             755..781
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6779..6900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6816..6835)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6870..6889
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6907..7362)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7044..7065)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7256..7275
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7389..7493
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7495..7852
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7495..7686
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7518..7538)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7703..7838
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7887..8681
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7887..8681
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7941..7960)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8551..8570
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8869..8888)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8913..8960
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9289..9877
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9778..9797
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
      721 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
      781 tcctccatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg atatggaaga
      841 cagaagagct gaaaagtcat gtgaacaagc atgtgaatca cttaagaggc aggactatga
      901 aatggccctc aagcactgca cagaggccct tctttctctt ggccagtact ccatggcaga
      961 cttcacaggg ccttgtccat tggaaataga acgcatcaaa atcgagagtc ttctctacag
     1021 aattgcctca tttttgcaac tgaaaaatta tgtgcaagct gatgaagatt gtagacatgt
     1081 gctgggagaa ggactggcca agggagaaga tgcctttcgg gcagtgcttt gctgcatgca
     1141 gctgaaaggg aagctccaac ctgtatccac cattcttgcc aagtcactca caggagagtc
     1201 cctgaatggg atggtaacaa aggatttgac aagactaaaa acacttctct cagaaacaga
     1261 gacagcaact agtaacgccc tctctggata tcacgtggaa gacttagatg aggggtcttg
     1321 taatggttgg catttccgcc caccacctag gggaatcaca agcagcgagg aatatacttt
     1381 gtgtaaaaga tttttagaac aaggaatctg taggtatggt gcccagtgta cttcagcaca
     1441 ttcccaggaa gaactagcag aatggcagaa aagatatgct tcacggctga taaaattgaa
     1501 acagcaaaat gagaataaac agctctcagg cagttacatg gaaaccttga tagaaaagtg
     1561 gatgaattca ttgtctcctg agaaagtgct tagtgaatgt atagaaggag taaaggtaga
     1621 gcacaatcct gacctgtcag ttactgtcag caccaaaaaa tcccaccaga catggacctt
     1681 tgctctcact tgtaagcctg caagaatgct gtatcgtgta gcattgcttt atgatgctca
     1741 tcgtcctcat tttagtatca ttgcaatatc tgccggagat agtactaccc aggtatcaca
     1801 agaagtccca gaaaactgtc aagaatggat aggaggaaag atggcccaaa atggattaga
     1861 tcattacgtg tataaagtcg ggatagcatt taacacagaa atatttggaa cttttcgcca
     1921 aaccatagtt ttcgactttg gattggaacc agtactcatg caaagagtaa tgattgatgc
     1981 agcttctaca gaagatctcg aatacctgat gcatgcaaaa cagcagctag taaccacagc
     2041 taaacgttgg gattcttcct ctaagactat tatagatttt gaacctaatg aaactactga
     2101 tttggagaag agccttctta tcagatacca aattcccctc tctgctgacc agctatttac
     2161 tcagtccgtt ttagacaaat cattgaccaa gagcaactat cagtcacggt tacatgacct
     2221 tctttatatt gaggagatag cccagtataa agaaatcagc aagttcaacc ttaaagtgca
     2281 attgcagatt ctggcaagct tcatgctcac tggtgtttct ggaggtgcaa agtatgctca
     2341 gaatggacaa ctttttggtc gctttaagct tactgaaaca ctttctgaag atactttggc
     2401 tggacgactg gtgatgacca aagtcaatgc tgtttattta ttaccagtcc ctaaacagaa
     2461 gttagtacag acccagggaa ccaaagagaa ggtttatgaa gctactattg aagaaaaaac
     2521 aaaggaatat atatttttaa ggctatctag ggaatgctgt gaagaactta atcttcggcc
     2581 tgactgtgac acacaggttg aacttcagtt tcaattaaat cgattacccc tctgtgaaat
     2641 gcactatgca ctagacagga tcaaggacaa tggggttttg tttccagaca tcagtatgac
     2701 tcccaccata ccatggagtc ctaacagaca atgggatgaa cagttggatc ctcgactaaa
     2761 tgcaaaacag aaagaggctg ttctggccat taccactcca cttgcaatcc agctgccgcc
     2821 tgtgcttatc atcggaccct atgggacagg caaaacgttc actctagctc aggctgtcaa
     2881 acatattctg cagcaacagg agactaggat tctcatttgc acccattcta atagtgctgc
     2941 tgatctctac ataaaggatt atttacatcc atatgtagaa gcaggcaatc cccaggcaag
     3001 acctctcagg gtatatttca gaaatcgctg ggtaaagact gtccacccag ttgtgcatca
     3061 gtactgtttg atctcaagcg cacattccac ctttcagatg ccccagaaag aagatattct
     3121 taaacatcga gtggtggttg ttaccttgaa tacttcccag tacctctgtc agttggacct
     3181 tgaacctggg ttttttacac acattctatt agatgaagct gcccaggcca tggagtgtga
     3241 aaccattatg cctctagcat tagcaactca aaacactcgg attgtcttgg ctggtgatca
     3301 catgcagctc agtccttttg tttacagcga gtttgccagg gagagaaacc ttcacgtttc
     3361 attacttgac cgactctatg agcattaccc tgctgagttc ccatgtagga ttctcctgtg
     3421 tgagaactac cgctcccatg aagctatcat caattatacc tctgagcttt tctatgaggg
     3481 caaactgatg gccagtggga agcagccagc acacaaagat ttctacccac taactttctt
     3541 tacagcacga ggagaagatg tacaagaaaa aaatagcaca gctttttata ataatgcaga
     3601 ggtgtttgaa gtggtggaac gtgtagaaga gttaagaagg aagtggccag tagcgtgggg
     3661 gaagttagat gatggcagta ttggtgtggt gactccatat gctgatcaag tgtttagaat
     3721 acgtgctgaa cttcgaaaaa agagattatc tgatgttaat gtagaaaggg tgctaaatgt
     3781 tcaaggaaag caattcagag ttttgtttct tagcacagta cgtacaagac atacttgtaa
     3841 acataaacag acaccaatta aaaagaaaga gcaacttctg gaagattcca cagaggactt
     3901 agattatggt tttttatcta actacaagct tctcaatact gccatcacaa gagcacaatc
     3961 cctggttgct gtggtgggtg atcccattgc tctgtgctct attggaagat gcaggaaatt
     4021 ttgggaacgg tttattgccc tgtgtcatga aaacagtagc ctacatggaa tcacttttga
     4081 acagatcaaa gcccagttag aggctttaga actaaagaag acatatgtgt tgaatccgct
     4141 ggcacctgaa tttatccccc gggctctaag actgcagcat tcaggaagta ccaacaaaca
     4201 gcagcaatca ccacccaagg ggaaaagtct tcatcatacc cagaatgatc acttccagaa
     4261 tgatggaatt gttcagccca atccttctgt acttattggc aatcctatta gagcatatac
     4321 tcctccaccc cctcttggac ctcacccaaa tttgggaaaa tctccaagcc ctgttcaaag
     4381 aatagatcct cacactggga caagtattct ttatgtacct gctgtctatg gagggaatgt
     4441 agttatgtcg gtgcctttac ctgtaccatg gacaggatac cagggtaggt ttgcagttga
     4501 tcctcgaatt attacacatc aggcagcaat ggcctataac atgaacctat tacagacaca
     4561 tggacgagga tctcctattc cttatggcct tggacatcac ccacctgtca ccataggcca
     4621 gccacaaaat cagcatcagg agaaggatca acatgagcaa aatcgaaatg gtaaaagtga
     4681 tacaaataat tccggacctg aaattaataa gattcgaaca ccagagaaaa agccaacaga
     4741 accaaaacag gttgatttgg aatcaaatcc acagaacaga agtcctgaat cacgtcctag
     4801 tgttgtttat cccagtacca aatttcctcg caaagataat ctcaacccaa gacacataaa
     4861 tcttcccctt cctgctcccc acgcacagta tgcaatccct aatcgccact ttcatcccct
     4921 tccccagcta ccaagaccac cctttccaat tccacagcag cacaccttgt taaatcagca
     4981 gcagaataat ttgcctgaac aaccaaatca gataccacct cagccaaatc aggtagtcca
     5041 gcagcaaagt cagttgaatc agcagcctca gcagccacct cctcagcttt ctcctgcata
     5101 tcaggcggga cccaacaatg ctttttttaa tagtgcagtt gctcatcggc cacagtctcc
     5161 tcctgcagaa gctgtaattc cggagcagca gccccctccc atgctgcaag aaggccacag
     5221 tcctctgaga gccattgcac aacccggccc cattcttcct tcacatctga atagcttcat
     5281 tgatgagaac ccctcgggat tacctatagg ggaggcttta gatcgtatac atgggagtgt
     5341 cgctctggaa acattaaggc agcagcaggc acggttccag cagtggagcg agcatcatgc
     5401 ctttctcagt cagggcagcg ttccataccc acaccatcac catcctcacc tccagcatct
     5461 tcctcagccg cccctgggat tacatcagcc gccagtgagg gcagactgga agctcaccag
     5521 cagtgccgaa gatgaagtgg agaccacata ctcaaggttt caagacttaa tcagagaact
     5581 gtctcatcgt gatcaaagtg aaacacggga actagctgaa atgccaccac ctcaatcaag
     5641 acttttgcaa tatagacaag tacagagtag aagcccacca gcagtcccat ctcccccatc
     5701 cagtacagac cacagtagcc acttttctaa ctttaatgat aacagcagag acattgaagt
     5761 agccagcaac ccagcatttc cacagcgcct cccaccccag atattcaact cacctttctc
     5821 gttgccatct gaacaccttg cccctcctcc cttgaaatac ctggcacctg atggagcatg
     5881 gacttttgct aacttgcaac agaatcacct aatggggcca ggttttccct atggcctacc
     5941 tccattgcct cacaggccac cgcagaaccc ttttgtacaa atacagaatc atcaacatgc
     6001 tattggtcaa gagccatttc acccattgtc atctcgaaca gtatcttctt cttcgctccc
     6061 tagcttagaa gagtatgagc ccagaggacc tggtcggccc ttgtaccaaa gaagaatctc
     6121 atctagctca gttcaacctt gttctgaaga agtaagcact cctcaagaca gtctggctca
     6181 gtgtaaagag cttcaggacc acagtaacca atcttctttc aacttttcat ccccggagtc
     6241 ctgggtaaac accacctcat ctactcctta tcagaacatt ccgtgcaatg gatccagcag
     6301 gacagctcag cccagagagt tgatagcgcc acccaagact gtcaaacccc ctgaggatca
     6361 actgaagtcg gagaacctcg aggtgtccag ttccttcaac tacagtgtgc tgcagcatct
     6421 tggccagttt ccacccctta tgcctaacaa gcagatcgcg gagtcggcca atagcagtag
     6481 cccccagagc tctgcggggg gcaagcccgc catgtcctat gccagcgctc tgcgggcccc
     6541 tccaaagccc aggccccctc ctgagcaggc caagaagagt agcgaccctc tgtctctctt
     6601 ccaggaactg agcctaggga gctcatctgg cagcaatggc ttttactcat attttaaata
     6661 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     6721 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     6781 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6841 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6901 acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     6961 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     7021 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     7081 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     7141 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     7201 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     7261 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     7321 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
     7381 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7441 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
     7501 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7561 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     7621 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     7681 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7741 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7801 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
     7861 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     7921 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     7981 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     8041 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     8101 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     8161 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     8221 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     8281 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     8341 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     8401 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     8461 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     8521 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     8581 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     8641 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     8701 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     8761 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     8821 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
     8881 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     8941 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     9001 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     9061 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     9121 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     9181 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     9241 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     9301 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     9361 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     9421 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     9481 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     9541 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     9601 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9661 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9721 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9781 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9841 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9901 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9961 ttctgtggat aaccgtatta ccgccatgca t
//
