LOCUS       sequence-291250-       10608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsHelz-F1107V.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10608)
  AUTHORS   Hanet A, Rasch F, Weber R, Ruscica V, Fauser M, Raisch T, 
            Kuzuoglu-Ozturk D, Chang CT, Bhandari D, Igreja C, Wohlbold L
  TITLE     HELZ directly interacts with CCR4-NOT and causes decay of bound 
            mRNAs.
  JOURNAL   Life Sci Alliance. 2019 Sep 30;2(5). pii: 2/5/e201900405. doi: 
            10.26508/lsa.201900405. Print 2019 Oct.
  PUBMED    31570513
REFERENCE   2  (bases 1 to 10608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2019-09-30"; volume: "2(5). pii: 2/5/e201900405. 
            doi"; pages: " 10.26508/lsa.201900405. Print 2019 Oct"
COMMENT     Sequence Label: pT7-EGFP-C1-HsHelz-F1107V_AG
FEATURES             Location/Qualifiers
     source          1..10608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(710..731)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(971..990)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1318..1339
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7396..7517
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7433..7452)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7487..7506
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7524..7979)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7661..7682)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7873..7892
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8006..8110
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8112..8469
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8112..8303
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8135..8155)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8320..8455
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8504..9298
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8504..9298
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8558..8577)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9168..9187
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9486..9505)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9530..9577
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9906..10494
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10395..10414
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      721 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      781 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      841 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      901 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      961 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1021 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1081 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1141 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1201 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1261 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1321 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1381 gtccggactc agatctcgag ctcaagcttc gaattctgca gtcgacatgg aagacagaag
     1441 agctgaaaag tcatgtgaac aagcatgtga atcacttaag aggcaggact atgaaatggc
     1501 cctcaagcac tgcacagagg cccttctttc tcttggccag tactccatgg cagacttcac
     1561 agggccttgt ccattggaaa tagaacgcat caaaatcgag agtcttctct acagaattgc
     1621 ctcatttttg caactgaaaa attatgtgca agctgatgaa gattgtagac atgtgctggg
     1681 agaaggactg gccaagggag aagatgcctt tcgggcagtg ctttgctgca tgcagctgaa
     1741 agggaagctc caacctgtat ccaccattct tgccaagtca ctcacaggag agtccctgaa
     1801 tgggatggta acaaaggatt tgacaagact aaaaacactt ctctcagaaa cagagacagc
     1861 aactagtaac gccctctctg gatatcacgt ggaagactta gatgaggggt cttgtaatgg
     1921 ttggcatttc cgcccaccac ctaggggaat cacaagcagc gaggaatata ctttgtgtaa
     1981 aagattttta gaacaaggaa tctgtaggta tggtgcccag tgtacttcag cacattccca
     2041 ggaagaacta gcagaatggc agaaaagata tgcttcacgg ctgataaaat tgaaacagca
     2101 aaatgagaat aaacagctct caggcagtta catggaaacc ttgatagaaa agtggatgaa
     2161 ttcattgtct cctgagaaag tgcttagtga atgtatagaa ggagtaaagg tagagcacaa
     2221 tcctgacctg tcagttactg tcagcaccaa aaaatcccac cagacatgga cctttgctct
     2281 cacttgtaag cctgcaagaa tgctgtatcg tgtagcattg ctttatgatg ctcatcgtcc
     2341 tcattttagt atcattgcaa tatctgccgg agatagtact acccaggtat cacaagaagt
     2401 cccagaaaac tgtcaagaat ggataggagg aaagatggcc caaaatggat tagatcatta
     2461 cgtgtataaa gtcgggatag catttaacac agaaatattt ggaacttttc gccaaaccat
     2521 agttttcgac tttggattgg aaccagtact catgcaaaga gtaatgattg atgcagcttc
     2581 tacagaagat ctcgaatacc tgatgcatgc aaaacagcag ctagtaacca cagctaaacg
     2641 ttgggattct tcctctaaga ctattataga ttttgaacct aatgaaacta ctgatttgga
     2701 gaagagcctt cttatcagat accaaattcc cctctctgct gaccagctat ttactcagtc
     2761 cgttttagac aaatcattga ccaagagcaa ctatcagtca cggttacatg accttcttta
     2821 tattgaggag atagcccagt ataaagaaat cagcaagttc aaccttaaag tgcaattgca
     2881 gattctggca agcttcatgc tcactggtgt ttctggaggt gcaaagtatg ctcagaatgg
     2941 acaacttttt ggtcgcttta agcttactga aacactttct gaagatactt tggctggacg
     3001 actggtgatg accaaagtca atgctgttta tttattacca gtccctaaac agaagttagt
     3061 acagacccag ggaaccaaag agaaggttta tgaagctact attgaagaaa aaacaaagga
     3121 atatatattt ttaaggctat ctagggaatg ctgtgaagaa cttaatcttc ggcctgactg
     3181 tgacacacag gttgaacttc agtttcaatt aaatcgatta cccctctgtg aaatgcacta
     3241 tgcactagac aggatcaagg acaatggggt tttgtttcca gacatcagta tgactcccac
     3301 cataccatgg agtcctaaca gacaatggga tgaacagttg gatcctcgac taaatgcaaa
     3361 acagaaagag gctgttctgg ccattaccac tccacttgca atccagctgc cgcctgtgct
     3421 tatcatcgga ccctatggga caggcaaaac gttcactcta gctcaggctg tcaaacatat
     3481 tctgcagcaa caggagacta ggattctcat ttgcacccat tctaatagtg ctgctgatct
     3541 ctacataaag gattatttac atccatatgt agaagcaggc aatccccagg caagacctct
     3601 cagggtatat ttcagaaatc gctgggtaaa gactgtccac ccagttgtgc atcagtactg
     3661 tttgatctca agcgcacatt ccacctttca gatgccccag aaagaagata ttcttaaaca
     3721 tcgagtggtg gttgttacct tgaatacttc ccagtacctc tgtcagttgg accttgaacc
     3781 tgggtttttt acacacattc tattagatga agctgcccag gccatggagt gtgaaaccat
     3841 tatgcctcta gcattagcaa ctcaaaacac tcggattgtc ttggctggtg atcacatgca
     3901 gctcagtcct tttgtttaca gcgagtttgc cagggagaga aaccttcacg tttcattact
     3961 tgaccgactc tatgagcatt accctgctga gttcccatgt aggattctcc tgtgtgagaa
     4021 ctaccgctcc catgaagcta tcatcaatta tacctctgag cttttctatg agggcaaact
     4081 gatggccagt gggaagcagc cagcacacaa agatttctac ccactaactt tctttacagc
     4141 acgaggagaa gatgtacaag aaaaaaatag cacagctttt tataataatg cagaggtgtt
     4201 tgaagtggtg gaacgtgtag aagagttaag aaggaagtgg ccagtagcgt gggggaagtt
     4261 agatgatggc agtattggtg tggtgactcc atatgctgat caagtgttta gaatacgtgc
     4321 tgaacttcga aaaaagagat tatctgatgt taatgtagaa agggtgctaa atgttcaagg
     4381 aaagcaattc agagttttgt ttcttagcac agtacgtaca agacatactt gtaaacataa
     4441 acagacacca attaaaaaga aagagcaact tctggaagat tccacagagg acttagatta
     4501 tggtttttta tctaactaca agcttctcaa tactgccatc acaagagcac aatccctggt
     4561 tgctgtggtg ggtgatccca ttgctctgtg ctctattgga agatgcagga aattttggga
     4621 acggtttatt gccctgtgtc atgaaaacag tagcctacat ggaatcactt ttgaacagat
     4681 caaagcccag ttagaggctt tagaactaaa gaagacatat gtgttgaatc cgctggcacc
     4741 tgaagttatc ccccgggctc taagactgca gcattcagga agtaccaaca aacagcagca
     4801 atcaccaccc aaggggaaaa gtcttcatca tacccagaat gatcacttcc agaatgatgg
     4861 aattgttcag cccaatcctt ctgtacttat tggcaatcct attagagcat atactcctcc
     4921 accccctctt ggacctcacc caaatttggg aaaatctcca agccctgttc aaagaataga
     4981 tcctcacact gggacaagta ttctttatgt acctgctgtc tatggaggga atgtagttat
     5041 gtcggtgcct ttacctgtac catggacagg ataccagggt aggtttgcag ttgatcctcg
     5101 aattattaca catcaggcag caatggccta taacatgaac ctattacaga cacatggacg
     5161 aggatctcct attccttatg gccttggaca tcacccacct gtcaccatag gccagccaca
     5221 aaatcagcat caggagaagg atcaacatga gcaaaatcga aatggtaaaa gtgatacaaa
     5281 taattccgga cctgaaatta ataagattcg aacaccagag aaaaagccaa cagaaccaaa
     5341 acaggttgat ttggaatcaa atccacagaa cagaagtcct gaatcacgtc ctagtgttgt
     5401 ttatcccagt accaaatttc ctcgcaaaga taatctcaac ccaagacaca taaatcttcc
     5461 ccttcctgct ccccacgcac agtatgcaat ccctaatcgc cactttcatc cccttcccca
     5521 gctaccaaga ccaccctttc caattccaca gcagcacacc ttgttaaatc agcagcagaa
     5581 taatttgcct gaacaaccaa atcagatacc acctcagcca aatcaggtag tccagcagca
     5641 aagtcagttg aatcagcagc ctcagcagcc acctcctcag ctttctcctg catatcaggc
     5701 gggacccaac aatgcttttt ttaatagtgc agttgctcat cggccacagt ctcctcctgc
     5761 agaagctgta attccggagc agcagccccc tcccatgctg caagaaggcc acagtcctct
     5821 gagagccatt gcacaacccg gccccattct tccttcacat ctgaatagct tcattgatga
     5881 gaacccctcg ggattaccta taggggaggc tttagatcgt atacatggga gtgtcgctct
     5941 ggaaacatta aggcagcagc aggcacggtt ccagcagtgg agcgagcatc atgcctttct
     6001 cagtcagggc agcgttccat acccacacca tcaccatcct cacctccagc atcttcctca
     6061 gccgcccctg ggattacatc agccgccagt gagggcagac tggaagctca ccagcagtgc
     6121 cgaagatgaa gtggagacca catactcaag gtttcaagac ttaatcagag aactgtctca
     6181 tcgtgatcaa agtgaaacac gggaactagc tgaaatgcca ccacctcaat caagactttt
     6241 gcaatataga caagtacaga gtagaagccc accagcagtc ccatctcccc catccagtac
     6301 agaccacagt agccactttt ctaactttaa tgataacagc agagacattg aagtagccag
     6361 caacccagca tttccacagc gcctcccacc ccagatattc aactcacctt tctcgttgcc
     6421 atctgaacac cttgcccctc ctcccttgaa atacctggca cctgatggag catggacttt
     6481 tgctaacttg caacagaatc acctaatggg gccaggtttt ccctatggcc tacctccatt
     6541 gcctcacagg ccaccgcaga acccttttgt acaaatacag aatcatcaac atgctattgg
     6601 tcaagagcca tttcacccat tgtcatctcg aacagtatct tcttcttcgc tccctagctt
     6661 agaagagtat gagcccagag gacctggtcg gcccttgtac caaagaagaa tctcatctag
     6721 ctcagttcaa ccttgttctg aagaagtaag cactcctcaa gacagtctgg ctcagtgtaa
     6781 agagcttcag gaccacagta accaatcttc tttcaacttt tcatccccgg agtcctgggt
     6841 aaacaccacc tcatctactc cttatcagaa cattccgtgc aatggatcca gcaggacagc
     6901 tcagcccaga gagttgatag cgccacccaa gactgtcaaa ccccctgagg atcaactgaa
     6961 gtcggagaac ctcgaggtgt ccagttcctt caactacagt gtgctgcagc atcttggcca
     7021 gtttccaccc cttatgccta acaagcagat cgcggagtcg gccaatagca gtagccccca
     7081 gagctctgcg gggggcaagc ccgccatgtc ctatgccagc gctctgcggg cccctccaaa
     7141 gcccaggccc cctcctgagc aggccaagaa gagtagcgac cctctgtctc tcttccagga
     7201 actgagccta gggagctcat ctggcagcaa tggcttttac tcatatttta aataaccgcg
     7261 ggcccgggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
     7321 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     7381 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7441 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7501 ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     7561 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     7621 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     7681 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     7741 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     7801 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     7861 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     7921 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     7981 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     8041 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8101 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     8161 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8221 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     8281 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     8341 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8401 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     8461 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     8521 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     8581 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     8641 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     8701 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     8761 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     8821 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     8881 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     8941 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     9001 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     9061 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     9121 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     9181 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     9241 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     9301 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     9361 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     9421 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     9481 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     9541 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     9601 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     9661 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     9721 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     9781 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9841 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9901 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9961 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    10021 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
    10081 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    10141 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    10201 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    10261 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    10321 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    10381 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    10441 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    10501 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    10561 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
