LOCUS       sequence-291255-        7183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsHelz_1051-1942.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Hanet A, Rasch F, Weber R, Ruscica V, Fauser M, Raisch T, 
            Kuzuoglu-Ozturk D, Chang CT, Bhandari D, Igreja C, Wohlbold L
  TITLE     HELZ directly interacts with CCR4-NOT and causes decay of bound 
            mRNAs.
  JOURNAL   Life Sci Alliance. 2019 Sep 30;2(5). pii: 2/5/e201900405. doi: 
            10.26508/lsa.201900405. Print 2019 Oct.
  PUBMED    31570513
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2019-09-30"; volume: "2(5). pii: 2/5/e201900405. 
            doi"; pages: " 10.26508/lsa.201900405. Print 2019 Oct"
COMMENT     Sequence Label: pT7-MS2-HA-HsHelz_1051-1942_AG
FEATURES             Location/Qualifiers
     source          1..7183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1097..1123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3971..4092
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4008..4027)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4062..4081
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4099..4554)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4236..4257)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4448..4467
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4581..4685
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4687..5044
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4687..4878
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4710..4730)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4895..5030
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5079..5873
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5079..5873
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5133..5152)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5743..5762
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6061..6080)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6105..6152
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6481..7069
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6970..6989
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggct tctaacttta ctcagttcgt tctcgtcgac aatggcggaa ctggcgacgt
      721 gactgtcgcc ccaagcaact tcgctaacgg ggtcgctgaa tggatcagct ctaactcgcg
      781 atcacaggct tacaaagtaa cctgtagcgt tcgtcagagc tctgcgcaga atcgcaaata
      841 caccatcaaa gtcgaggtgc ctaaagtggc aacccagact gttggtggtg aagagcttcc
      901 tgtagccgga tggagatctt acttaaatat ggaactaacc attccaattt tcgccacgaa
      961 ttccgactgc gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat
     1021 tccctcggcc atcgcggcca actccggcat ctacggaggt ggaggtggat ctggtggatc
     1081 tcctccacat atggcttacc catacgatgt tccagattac gctcctccat ctcgagctca
     1141 agcttcgaat tctgcagtcg acggtaccgc ggatcccatt gctctgtgct ctattggaag
     1201 atgcaggaaa ttttgggaac ggtttattgc cctgtgtcat gaaaacagta gcctacatgg
     1261 aatcactttt gaacagatca aagcccagtt agaggcttta gaactaaaga agacatatgt
     1321 gttgaatccg ctggcacctg aatttatccc ccgggctcta agactgcagc attcaggaag
     1381 taccaacaaa cagcagcaat caccacccaa ggggaaaagt cttcatcata cccagaatga
     1441 tcacttccag aatgatggaa ttgttcagcc caatccttct gtacttattg gcaatcctat
     1501 tagagcatat actcctccac cccctcttgg acctcaccca aatttgggaa aatctccaag
     1561 ccctgttcaa agaatagatc ctcacactgg gacaagtatt ctttatgtac ctgctgtcta
     1621 tggagggaat gtagttatgt cggtgccttt acctgtacca tggacaggat accagggtag
     1681 gtttgcagtt gatcctcgaa ttattacaca tcaggcagca atggcctata acatgaacct
     1741 attacagaca catggacgag gatctcctat tccttatggc cttggacatc acccacctgt
     1801 caccataggc cagccacaaa atcagcatca ggagaaggat caacatgagc aaaatcgaaa
     1861 tggtaaaagt gatacaaata attccggacc tgaaattaat aagattcgaa caccagagaa
     1921 aaagccaaca gaaccaaaac aggttgattt ggaatcaaat ccacagaaca gaagtcctga
     1981 atcacgtcct agtgttgttt atcccagtac caaatttcct cgcaaagata atctcaaccc
     2041 aagacacata aatcttcccc ttcctgctcc ccacgcacag tatgcaatcc ctaatcgcca
     2101 ctttcatccc cttccccagc taccaagacc accctttcca attccacagc agcacacctt
     2161 gttaaatcag cagcagaata atttgcctga acaaccaaat cagataccac ctcagccaaa
     2221 tcaggtagtc cagcagcaaa gtcagttgaa tcagcagcct cagcagccac ctcctcagct
     2281 ttctcctgca tatcaggcgg gacccaacaa tgcttttttt aatagtgcag ttgctcatcg
     2341 gccacagtct cctcctgcag aagctgtaat tccggagcag cagccccctc ccatgctgca
     2401 agaaggccac agtcctctga gagccattgc acaacccggc cccattcttc cttcacatct
     2461 gaatagcttc attgatgaga acccctcggg attacctata ggggaggctt tagatcgtat
     2521 acatgggagt gtcgctctgg aaacattaag gcagcagcag gcacggttcc agcagtggag
     2581 cgagcatcat gcctttctca gtcagggcag cgttccatac ccacaccatc accatcctca
     2641 cctccagcat cttcctcagc cgcccctggg attacatcag ccgccagtga gggcagactg
     2701 gaagctcacc agcagtgccg aagatgaagt ggagaccaca tactcaaggt ttcaagactt
     2761 aatcagagaa ctgtctcatc gtgatcaaag tgaaacacgg gaactagctg aaatgccacc
     2821 acctcaatca agacttttgc aatatagaca agtacagagt agaagcccac cagcagtccc
     2881 atctccccca tccagtacag accacagtag ccacttttct aactttaatg ataacagcag
     2941 agacattgaa gtagccagca acccagcatt tccacagcgc ctcccacccc agatattcaa
     3001 ctcacctttc tcgttgccat ctgaacacct tgcccctcct cccttgaaat acctggcacc
     3061 tgatggagca tggacttttg ctaacttgca acagaatcac ctaatggggc caggttttcc
     3121 ctatggccta cctccattgc ctcacaggcc accgcagaac ccttttgtac aaatacagaa
     3181 tcatcaacat gctattggtc aagagccatt tcacccattg tcatctcgaa cagtatcttc
     3241 ttcttcgctc cctagcttag aagagtatga gcccagagga cctggtcggc ccttgtacca
     3301 aagaagaatc tcatctagct cagttcaacc ttgttctgaa gaagtaagca ctcctcaaga
     3361 cagtctggct cagtgtaaag agcttcagga ccacagtaac caatcttctt tcaacttttc
     3421 atccccggag tcctgggtaa acaccacctc atctactcct tatcagaaca ttccgtgcaa
     3481 tggatccagc aggacagctc agcccagaga gttgatagcg ccacccaaga ctgtcaaacc
     3541 ccctgaggat caactgaagt cggagaacct cgaggtgtcc agttccttca actacagtgt
     3601 gctgcagcat cttggccagt ttccacccct tatgcctaac aagcagatcg cggagtcggc
     3661 caatagcagt agcccccaga gctctgcggg gggcaagccc gccatgtcct atgccagcgc
     3721 tctgcgggcc cctccaaagc ccaggccccc tcctgagcag gccaagaaga gtagcgaccc
     3781 tctgtctctc ttccaggaac tgagcctagg gagctcatct ggcagcaatg gcttttactc
     3841 atattttaaa taatctagat aactgatcat aatcagccat accacatttg tagaggtttt
     3901 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     3961 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4021 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4081 caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     4141 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     4201 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     4261 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     4321 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     4381 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     4441 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     4501 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     4561 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4621 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4681 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4741 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4801 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4861 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4921 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4981 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5041 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     5101 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     5161 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     5221 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     5281 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     5341 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     5401 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     5461 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     5521 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     5581 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     5641 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     5701 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     5761 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     5821 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     5881 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     5941 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     6001 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     6061 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     6121 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     6181 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     6241 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     6301 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
     6361 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6421 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6481 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6541 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6601 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     6661 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6721 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6781 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6841 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6901 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6961 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7021 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7081 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7141 gttatcccct gattctgtgg ataaccgtat taccgccatg cat
//
