LOCUS       sequence-291258-       10333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsHelz-F1107V.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10333)
  AUTHORS   Hanet A, Rasch F, Weber R, Ruscica V, Fauser M, Raisch T, 
            Kuzuoglu-Ozturk D, Chang CT, Bhandari D, Igreja C, Wohlbold L
  TITLE     HELZ directly interacts with CCR4-NOT and causes decay of bound 
            mRNAs.
  JOURNAL   Life Sci Alliance. 2019 Sep 30;2(5). pii: 2/5/e201900405. doi: 
            10.26508/lsa.201900405. Print 2019 Oct.
  PUBMED    31570513
REFERENCE   2  (bases 1 to 10333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2019-09-30"; volume: "2(5). pii: 2/5/e201900405. 
            doi"; pages: " 10.26508/lsa.201900405. Print 2019 Oct"
COMMENT     Sequence Label: pT7-MS2-HA-HsHelz-F1107V_AG
FEATURES             Location/Qualifiers
     source          1..10333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1097..1123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    7121..7242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7158..7177)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7212..7231
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7249..7704)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7386..7407)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7598..7617
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7731..7835
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7837..8194
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7837..8028
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7860..7880)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8045..8180
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8229..9023
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8229..9023
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8283..8302)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8893..8912
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9211..9230)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9255..9302
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9631..10219
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10120..10139
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggct tctaacttta ctcagttcgt tctcgtcgac aatggcggaa ctggcgacgt
      721 gactgtcgcc ccaagcaact tcgctaacgg ggtcgctgaa tggatcagct ctaactcgcg
      781 atcacaggct tacaaagtaa cctgtagcgt tcgtcagagc tctgcgcaga atcgcaaata
      841 caccatcaaa gtcgaggtgc ctaaagtggc aacccagact gttggtggtg aagagcttcc
      901 tgtagccgga tggagatctt acttaaatat ggaactaacc attccaattt tcgccacgaa
      961 ttccgactgc gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat
     1021 tccctcggcc atcgcggcca actccggcat ctacggaggt ggaggtggat ctggtggatc
     1081 tcctccacat atggcttacc catacgatgt tccagattac gctcctccat ctcgagctca
     1141 agcttcgaat tctgcagtcg acggtaccgc ggatatggaa gacagaagag ctgaaaagtc
     1201 atgtgaacaa gcatgtgaat cacttaagag gcaggactat gaaatggccc tcaagcactg
     1261 cacagaggcc cttctttctc ttggccagta ctccatggca gacttcacag ggccttgtcc
     1321 attggaaata gaacgcatca aaatcgagag tcttctctac agaattgcct catttttgca
     1381 actgaaaaat tatgtgcaag ctgatgaaga ttgtagacat gtgctgggag aaggactggc
     1441 caagggagaa gatgcctttc gggcagtgct ttgctgcatg cagctgaaag ggaagctcca
     1501 acctgtatcc accattcttg ccaagtcact cacaggagag tccctgaatg ggatggtaac
     1561 aaaggatttg acaagactaa aaacacttct ctcagaaaca gagacagcaa ctagtaacgc
     1621 cctctctgga tatcacgtgg aagacttaga tgaggggtct tgtaatggtt ggcatttccg
     1681 cccaccacct aggggaatca caagcagcga ggaatatact ttgtgtaaaa gatttttaga
     1741 acaaggaatc tgtaggtatg gtgcccagtg tacttcagca cattcccagg aagaactagc
     1801 agaatggcag aaaagatatg cttcacggct gataaaattg aaacagcaaa atgagaataa
     1861 acagctctca ggcagttaca tggaaacctt gatagaaaag tggatgaatt cattgtctcc
     1921 tgagaaagtg cttagtgaat gtatagaagg agtaaaggta gagcacaatc ctgacctgtc
     1981 agttactgtc agcaccaaaa aatcccacca gacatggacc tttgctctca cttgtaagcc
     2041 tgcaagaatg ctgtatcgtg tagcattgct ttatgatgct catcgtcctc attttagtat
     2101 cattgcaata tctgccggag atagtactac ccaggtatca caagaagtcc cagaaaactg
     2161 tcaagaatgg ataggaggaa agatggccca aaatggatta gatcattacg tgtataaagt
     2221 cgggatagca tttaacacag aaatatttgg aacttttcgc caaaccatag ttttcgactt
     2281 tggattggaa ccagtactca tgcaaagagt aatgattgat gcagcttcta cagaagatct
     2341 cgaatacctg atgcatgcaa aacagcagct agtaaccaca gctaaacgtt gggattcttc
     2401 ctctaagact attatagatt ttgaacctaa tgaaactact gatttggaga agagccttct
     2461 tatcagatac caaattcccc tctctgctga ccagctattt actcagtccg ttttagacaa
     2521 atcattgacc aagagcaact atcagtcacg gttacatgac cttctttata ttgaggagat
     2581 agcccagtat aaagaaatca gcaagttcaa ccttaaagtg caattgcaga ttctggcaag
     2641 cttcatgctc actggtgttt ctggaggtgc aaagtatgct cagaatggac aactttttgg
     2701 tcgctttaag cttactgaaa cactttctga agatactttg gctggacgac tggtgatgac
     2761 caaagtcaat gctgtttatt tattaccagt ccctaaacag aagttagtac agacccaggg
     2821 aaccaaagag aaggtttatg aagctactat tgaagaaaaa acaaaggaat atatattttt
     2881 aaggctatct agggaatgct gtgaagaact taatcttcgg cctgactgtg acacacaggt
     2941 tgaacttcag tttcaattaa atcgattacc cctctgtgaa atgcactatg cactagacag
     3001 gatcaaggac aatggggttt tgtttccaga catcagtatg actcccacca taccatggag
     3061 tcctaacaga caatgggatg aacagttgga tcctcgacta aatgcaaaac agaaagaggc
     3121 tgttctggcc attaccactc cacttgcaat ccagctgccg cctgtgctta tcatcggacc
     3181 ctatgggaca ggcaaaacgt tcactctagc tcaggctgtc aaacatattc tgcagcaaca
     3241 ggagactagg attctcattt gcacccattc taatagtgct gctgatctct acataaagga
     3301 ttatttacat ccatatgtag aagcaggcaa tccccaggca agacctctca gggtatattt
     3361 cagaaatcgc tgggtaaaga ctgtccaccc agttgtgcat cagtactgtt tgatctcaag
     3421 cgcacattcc acctttcaga tgccccagaa agaagatatt cttaaacatc gagtggtggt
     3481 tgttaccttg aatacttccc agtacctctg tcagttggac cttgaacctg ggttttttac
     3541 acacattcta ttagatgaag ctgcccaggc catggagtgt gaaaccatta tgcctctagc
     3601 attagcaact caaaacactc ggattgtctt ggctggtgat cacatgcagc tcagtccttt
     3661 tgtttacagc gagtttgcca gggagagaaa ccttcacgtt tcattacttg accgactcta
     3721 tgagcattac cctgctgagt tcccatgtag gattctcctg tgtgagaact accgctccca
     3781 tgaagctatc atcaattata cctctgagct tttctatgag ggcaaactga tggccagtgg
     3841 gaagcagcca gcacacaaag atttctaccc actaactttc tttacagcac gaggagaaga
     3901 tgtacaagaa aaaaatagca cagcttttta taataatgca gaggtgtttg aagtggtgga
     3961 acgtgtagaa gagttaagaa ggaagtggcc agtagcgtgg gggaagttag atgatggcag
     4021 tattggtgtg gtgactccat atgctgatca agtgtttaga atacgtgctg aacttcgaaa
     4081 aaagagatta tctgatgtta atgtagaaag ggtgctaaat gttcaaggaa agcaattcag
     4141 agttttgttt cttagcacag tacgtacaag acatacttgt aaacataaac agacaccaat
     4201 taaaaagaaa gagcaacttc tggaagattc cacagaggac ttagattatg gttttttatc
     4261 taactacaag cttctcaata ctgccatcac aagagcacaa tccctggttg ctgtggtggg
     4321 tgatcccatt gctctgtgct ctattggaag atgcaggaaa ttttgggaac ggtttattgc
     4381 cctgtgtcat gaaaacagta gcctacatgg aatcactttt gaacagatca aagcccagtt
     4441 agaggcttta gaactaaaga agacatatgt gttgaatccg ctggcacctg aagttatccc
     4501 ccgggctcta agactgcagc attcaggaag taccaacaaa cagcagcaat caccacccaa
     4561 ggggaaaagt cttcatcata cccagaatga tcacttccag aatgatggaa ttgttcagcc
     4621 caatccttct gtacttattg gcaatcctat tagagcatat actcctccac cccctcttgg
     4681 acctcaccca aatttgggaa aatctccaag ccctgttcaa agaatagatc ctcacactgg
     4741 gacaagtatt ctttatgtac ctgctgtcta tggagggaat gtagttatgt cggtgccttt
     4801 acctgtacca tggacaggat accagggtag gtttgcagtt gatcctcgaa ttattacaca
     4861 tcaggcagca atggcctata acatgaacct attacagaca catggacgag gatctcctat
     4921 tccttatggc cttggacatc acccacctgt caccataggc cagccacaaa atcagcatca
     4981 ggagaaggat caacatgagc aaaatcgaaa tggtaaaagt gatacaaata attccggacc
     5041 tgaaattaat aagattcgaa caccagagaa aaagccaaca gaaccaaaac aggttgattt
     5101 ggaatcaaat ccacagaaca gaagtcctga atcacgtcct agtgttgttt atcccagtac
     5161 caaatttcct cgcaaagata atctcaaccc aagacacata aatcttcccc ttcctgctcc
     5221 ccacgcacag tatgcaatcc ctaatcgcca ctttcatccc cttccccagc taccaagacc
     5281 accctttcca attccacagc agcacacctt gttaaatcag cagcagaata atttgcctga
     5341 acaaccaaat cagataccac ctcagccaaa tcaggtagtc cagcagcaaa gtcagttgaa
     5401 tcagcagcct cagcagccac ctcctcagct ttctcctgca tatcaggcgg gacccaacaa
     5461 tgcttttttt aatagtgcag ttgctcatcg gccacagtct cctcctgcag aagctgtaat
     5521 tccggagcag cagccccctc ccatgctgca agaaggccac agtcctctga gagccattgc
     5581 acaacccggc cccattcttc cttcacatct gaatagcttc attgatgaga acccctcggg
     5641 attacctata ggggaggctt tagatcgtat acatgggagt gtcgctctgg aaacattaag
     5701 gcagcagcag gcacggttcc agcagtggag cgagcatcat gcctttctca gtcagggcag
     5761 cgttccatac ccacaccatc accatcctca cctccagcat cttcctcagc cgcccctggg
     5821 attacatcag ccgccagtga gggcagactg gaagctcacc agcagtgccg aagatgaagt
     5881 ggagaccaca tactcaaggt ttcaagactt aatcagagaa ctgtctcatc gtgatcaaag
     5941 tgaaacacgg gaactagctg aaatgccacc acctcaatca agacttttgc aatatagaca
     6001 agtacagagt agaagcccac cagcagtccc atctccccca tccagtacag accacagtag
     6061 ccacttttct aactttaatg ataacagcag agacattgaa gtagccagca acccagcatt
     6121 tccacagcgc ctcccacccc agatattcaa ctcacctttc tcgttgccat ctgaacacct
     6181 tgcccctcct cccttgaaat acctggcacc tgatggagca tggacttttg ctaacttgca
     6241 acagaatcac ctaatggggc caggttttcc ctatggccta cctccattgc ctcacaggcc
     6301 accgcagaac ccttttgtac aaatacagaa tcatcaacat gctattggtc aagagccatt
     6361 tcacccattg tcatctcgaa cagtatcttc ttcttcgctc cctagcttag aagagtatga
     6421 gcccagagga cctggtcggc ccttgtacca aagaagaatc tcatctagct cagttcaacc
     6481 ttgttctgaa gaagtaagca ctcctcaaga cagtctggct cagtgtaaag agcttcagga
     6541 ccacagtaac caatcttctt tcaacttttc atccccggag tcctgggtaa acaccacctc
     6601 atctactcct tatcagaaca ttccgtgcaa tggatccagc aggacagctc agcccagaga
     6661 gttgatagcg ccacccaaga ctgtcaaacc ccctgaggat caactgaagt cggagaacct
     6721 cgaggtgtcc agttccttca actacagtgt gctgcagcat cttggccagt ttccacccct
     6781 tatgcctaac aagcagatcg cggagtcggc caatagcagt agcccccaga gctctgcggg
     6841 gggcaagccc gccatgtcct atgccagcgc tctgcgggcc cctccaaagc ccaggccccc
     6901 tcctgagcag gccaagaaga gtagcgaccc tctgtctctc ttccaggaac tgagcctagg
     6961 gagctcatct ggcagcaatg gcttttactc atattttaaa taatctagat aactgatcat
     7021 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     7081 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     7141 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7201 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg
     7261 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     7321 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     7381 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     7441 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     7501 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     7561 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     7621 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     7681 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7741 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7801 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     7861 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7921 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     7981 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     8041 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8101 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8161 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     8221 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     8281 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     8341 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     8401 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     8461 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     8521 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     8581 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     8641 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     8701 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     8761 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     8821 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     8881 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     8941 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     9001 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     9061 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     9121 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     9181 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     9241 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     9301 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     9361 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     9421 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     9481 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9541 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9601 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9661 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9721 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     9781 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9841 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9901 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9961 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    10021 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    10081 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    10141 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    10201 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    10261 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    10321 taccgccatg cat
//
