LOCUS       sequence-291313-        6394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsZNF598_273-904-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6394)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-MS2-HA-C1-HsZNF598_273-904-siRNAres_AH
FEATURES             Location/Qualifiers
     source          1..6394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1097..1123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3182..3303
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3219..3238)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3273..3292
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3310..3765)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3447..3468)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3659..3678
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3792..3896
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3898..4255
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3898..4089
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3921..3941)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4106..4241
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4290..5084
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4290..5084
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4344..4363)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4954..4973
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5272..5291)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5316..5363
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5692..6280
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6181..6200
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggct tctaacttta ctcagttcgt tctcgtcgac aatggcggaa ctggcgacgt
      721 gactgtcgcc ccaagcaact tcgctaacgg ggtcgctgaa tggatcagct ctaactcgcg
      781 atcacaggct tacaaagtaa cctgtagcgt tcgtcagagc tctgcgcaga atcgcaaata
      841 caccatcaaa gtcgaggtgc ctaaagtggc aacccagact gttggtggtg aagagcttcc
      901 tgtagccgga tggagatctt acttaaatat ggaactaacc attccaattt tcgccacgaa
      961 ttccgactgc gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat
     1021 tccctcggcc atcgcggcca actccggcat ctacggaggt ggaggtggat ctggtggatc
     1081 tcctccacat atggcttacc catacgatgt tccagattac gctcctccat ctcgagctag
     1141 ccgcgccgag gcacgccaga accgccacat cgacctgcag ttcagctacg cgccacggca
     1201 ctcgcgccgg aacgaggggg tcgttggtgg cgaagactac gaggaggtgg acaggtacag
     1261 ccgccagggc cgagtggccc gggctggcac tcgcggagcc cagcagagcc gccgaggaag
     1321 ctggaggtac aaaagggaag aagaggaccg agaagtagca gctgctgtcc gggcctccgt
     1381 ggccgcacag cagcaggagg aggcccgaag aagcgaagac caggaggaag gcggtaggcc
     1441 aaaaaaggaa gaagcggcgg cgcggggacc tgaggatccc cgtggccccc ggcgctcacc
     1501 ccggactcag ggcgaaggcc caggccccaa ggaaacctcg acaaatggtc ctgtaagcca
     1561 agaagccttc tcggtgacag gcccagccgc cccagggtgt gtgggggtgc caggcgccct
     1621 cccaccaccc agcccgaagc tcaaggacga agacttcccc agcctctctg cctccacttc
     1681 ctcctcctgc tccactgcag caaccccggg ccctgtgggg ttggcgctgc cgtacgccat
     1741 ccctgccaga ggcaggagtg ccttccagga ggaggacttc cccgccctgg tgtcctcggt
     1801 gcccaagcct ggcaccgccc ccaccagcct tgtctctgcc tggaacagca gcagtagcag
     1861 caagaaggta gcacagcccc cactctcggc gcaggctacc ggcagcggcc agcccaccag
     1921 gaaggctggg aaggggagca ggggcggcag gaagggcggc ccgcccttca cacaggagga
     1981 ggaggaggac ggcggcccgg ccctgcagga gcttctgagc acacgcccca cgggctccgt
     2041 ctcctccaca ctggggctgg cctccatcca accctctaaa gttgggaaga agaagaaagt
     2101 gggctcggag aagccaggca ccacattgcc acagcccccg cccgctacct gtcccccagg
     2161 ggctttgcag gccccggaag ctcctgccag cagagccgag gggccagttg ccgtcgtcgt
     2221 taatggacac acggagggcc cggcccctgc tcggagtgcc cccaaggaac cccctgggct
     2281 cccaaggccc ctggggtcct tcccctgccc cacgccacag gaggacttcc cagcgctcgg
     2341 cggcccctgc ccaccccgga tgccgccgcc cccaggcttc agcgctgtgg tgctcctgaa
     2401 gggcacgcct cccccacccc cgccgggcct ggtgccccca atcagcaagc cgccccctgg
     2461 cttctctggc cttctgccta gcccccaccc ggcctgtgtc cccagccccg ccaccaccac
     2521 caccacaaaa gcacccaggc tgctgcctgc cccacgggcg tacctagtcc ccgagaactt
     2581 ccgggagagg aaccttcagc tcatccagtc catcagggac ttcctgcaga gcgacgaggc
     2641 ccgcttcagc gagttcaaga gccactcagg ggagttcaga cagggcctga tctccgcagc
     2701 ccagtattac aagagttgcc gggacctgct gggggagaat ttccagaagg tctttaatga
     2761 gctgctggtc ctgctgcccg acacggccaa gcagcaggag ctcctgtctg cacacacgga
     2821 cttctgcaac cgcgagaagc ctctgagcac caagtccaag aagaacaaga agagcgcgtg
     2881 gcaggccacc acccagcagg cgggcctgga ctgccgtgtg tgccccacct gccagcaggt
     2941 gctcgcgcat ggcgatgcca gcagccacca ggcgctgcat gctgcccggg acgacgactt
     3001 cccctccctg caagccatcg ccaggatcat cacgtagggt accgcgggcc cgggatccac
     3061 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt
     3121 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     3181 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3241 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3301 ttaacgcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     3361 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     3421 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     3481 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     3541 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     3601 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     3661 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     3721 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     3781 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     3841 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg
     3901 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     3961 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     4021 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4081 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4141 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     4201 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
     4261 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     4321 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     4381 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     4441 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     4501 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     4561 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     4621 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     4681 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     4741 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     4801 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     4861 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     4921 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     4981 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     5041 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     5101 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     5161 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     5221 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
     5281 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     5341 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     5401 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     5461 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     5521 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
     5581 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5641 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5701 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5761 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5821 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5881 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5941 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6001 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6061 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6121 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6181 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6241 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6301 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     6361 tgattctgtg gataaccgta ttaccgccat gcat
//
