LOCUS       sequence-291318-        6914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsZNF598-del611-714-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6914)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 6914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-MS2-HA-C1-HsZNF598-del611-714-siRNAres_AH
FEATURES             Location/Qualifiers
     source          1..6914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1097..1123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3702..3823
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3739..3758)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3793..3812
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3830..4285)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3967..3988)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4179..4198
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4312..4416
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4418..4775
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4418..4609
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4441..4461)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4626..4761
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4810..5604
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4810..5604
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4864..4883)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5474..5493
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5792..5811)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5836..5883
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6212..6800
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6701..6720
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggct tctaacttta ctcagttcgt tctcgtcgac aatggcggaa ctggcgacgt
      721 gactgtcgcc ccaagcaact tcgctaacgg ggtcgctgaa tggatcagct ctaactcgcg
      781 atcacaggct tacaaagtaa cctgtagcgt tcgtcagagc tctgcgcaga atcgcaaata
      841 caccatcaaa gtcgaggtgc ctaaagtggc aacccagact gttggtggtg aagagcttcc
      901 tgtagccgga tggagatctt acttaaatat ggaactaacc attccaattt tcgccacgaa
      961 ttccgactgc gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat
     1021 tccctcggcc atcgcggcca actccggcat ctacggaggt ggaggtggat ctggtggatc
     1081 tcctccacat atggcttacc catacgatgt tccagattac gctcctccat ctcgagctat
     1141 ggcggcggcg gggggcgccg aggggcggcg cgcggccctg gaggcggcgg cggcggcagc
     1201 tcctgagcgg ggaggcggga gctgcgtgct gtgctgcgga gacctggagg ccacggcgct
     1261 gggccgctgc gaccacccgg tgtgctaccg ctgctctacc aagatgcggg tgctctgcga
     1321 gcagcgctac tgcgccgtgt gccgcgagga gctgcgccag gtggtctttg ggaagaagct
     1381 tcctgccttt gccaccatcc ccatccacca gctgcagcat gagaagaaat atgatatcta
     1441 ctttgcagat ggaaaggtgt acgcattgta caggcagctg ctgcagcacg agtgcccgcg
     1501 gtgccccgag ctgccacctt tcagcctctt cggggacctg gagcagcaca tgcggaggca
     1561 gcatgagctc ttctgctgcc gtctgtgcct ccagcacctc cagatcttca catatgagcg
     1621 caagtggtac tcgcgcaagg acctggcccg gcatcgcatg cagggtgacc ccgatgacac
     1681 gtcgcaccgt gggcacccgc tctgcaagtt ctgtgacgag cgctacctgg acaatgatga
     1741 gctgcttaag cacctgcgcc gcgaccacta cttctgccac ttctgcgact cggacggggc
     1801 ccaggactac tacagcgact atgcctacct gcgtgagcac ttccgggaga agcactttct
     1861 gtgtgaggaa ggccgctgca gcacggagca gttcacccac gccttccgca ccgagatcga
     1921 cctcaaggcc cacaggacgg cctgccacag tcgcagccgc gccgaggcac gccagaaccg
     1981 ccacatcgac ctgcagttca gctacgcgcc acggcactcg cgccggaacg agggggtcgt
     2041 tggtggcgaa gactacgagg aggtggacag gtacagccgc cagggccgag tggcccgggc
     2101 tggcactcgc ggagcccagc agagccgccg aggaagctgg aggtacaaaa gggaagaaga
     2161 ggaccgagaa gtagcagctg ctgtccgggc ctccgtggcc gcacagcagc aggaggaggc
     2221 ccgaagaagc gaagaccagg aggaaggcgg taggccaaaa aaggaagaag cggcggcgcg
     2281 gggacctgag gatccccgtg gcccccggcg ctcaccccgg actcagggcg aaggcccagg
     2341 ccccaaggaa acctcgacaa atggtcctgt aagccaagaa gccttctcgg tgacaggccc
     2401 agccgcccca gggtgtgtgg gggtgccagg cgccctccca ccacccagcc cgaagctcaa
     2461 ggacgaagac ttccccagcc tctctgcctc cacttcctcc tcctgctcca ctgcagcaac
     2521 cccgggccct gtggggttgg cgctgccgta cgccatccct gccagaggca ggagtgcctt
     2581 ccaggaggag gacttccccg ccctggtgtc ctcggtgccc aagcctggca ccgcccccac
     2641 cagccttgtc tctgcctgga acagcagcag tagcagcaag aaggtagcac agcccccact
     2701 ctcggcgcag gctaccggca gcggccagcc caccaggaag gctgggaagg ggagcagggg
     2761 cggcaggaag ggcggcccgc ccttcacaca ggaggaggag gaggacggcg gcccggccct
     2821 gcaggagctt ctgagcacac gccccacggg ctccgtctcc tccacactgg ggctggcctc
     2881 catccaaccc tctaaagttg ggaagaagaa gaaagtgggc tcggagaagc caggcaccac
     2941 attgccacag cccccgcccg ctacctgttc tggccttctg cctagccccc acccggcctg
     3001 tgtccccagc cccgccacca ccaccaccac aaaagcaccc aggctgctgc ctgccccacg
     3061 ggcgtaccta gtccccgaga acttccggga gaggaacctt cagctcatcc agtccatcag
     3121 ggacttcctg cagagcgacg aggcccgctt cagcgagttc aagagccact caggggagtt
     3181 cagacagggc ctgatctccg cagcccagta ttacaagagt tgccgggacc tgctggggga
     3241 gaatttccag aaggtcttta atgagctgct ggtcctgctg cccgacacgg ccaagcagca
     3301 ggagctcctg tctgcacaca cggacttctg caaccgcgag aagcctctga gcaccaagtc
     3361 caagaagaac aagaagagcg cgtggcaggc caccacccag caggcgggcc tggactgccg
     3421 tgtgtgcccc acctgccagc aggtgctcgc gcatggcgat gccagcagcc accaggcgct
     3481 gcatgctgcc cgggacgacg acttcccctc cctgcaagcc atcgccagga tcatcacgta
     3541 ggaattctgc agtcgacggt accgcgggcc cgggatccac cggatctaga taactgatca
     3601 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     3661 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     3721 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3781 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta aattgtaagc
     3841 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     3901 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     3961 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     4021 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     4081 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     4141 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     4201 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     4261 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4321 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4381 tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt
     4441 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4501 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     4561 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     4621 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     4681 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     4741 gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga
     4801 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     4861 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     4921 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     4981 aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     5041 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     5101 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     5161 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     5221 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     5281 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc
     5341 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     5401 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     5461 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     5521 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     5581 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     5641 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     5701 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     5761 agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
     5821 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg
     5881 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
     5941 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
     6001 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact
     6061 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6121 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6181 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6241 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6301 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     6361 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6421 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6481 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6541 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6601 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6661 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6721 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6781 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6841 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6901 ttaccgccat gcat
//
