LOCUS       sequence-291321-        7226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsZNF598-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7226)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-MS2-HA-C1-HsZNF598-siRNAres_AH
FEATURES             Location/Qualifiers
     source          1..7226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1097..1123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4014..4135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4051..4070)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4105..4124
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4142..4597)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4279..4300)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4491..4510
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4624..4728
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4730..5087
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4730..4921
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4753..4773)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4938..5073
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5122..5916
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5122..5916
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5176..5195)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5786..5805
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6104..6123)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6148..6195
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6524..7112
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7013..7032
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggct tctaacttta ctcagttcgt tctcgtcgac aatggcggaa ctggcgacgt
      721 gactgtcgcc ccaagcaact tcgctaacgg ggtcgctgaa tggatcagct ctaactcgcg
      781 atcacaggct tacaaagtaa cctgtagcgt tcgtcagagc tctgcgcaga atcgcaaata
      841 caccatcaaa gtcgaggtgc ctaaagtggc aacccagact gttggtggtg aagagcttcc
      901 tgtagccgga tggagatctt acttaaatat ggaactaacc attccaattt tcgccacgaa
      961 ttccgactgc gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat
     1021 tccctcggcc atcgcggcca actccggcat ctacggaggt ggaggtggat ctggtggatc
     1081 tcctccacat atggcttacc catacgatgt tccagattac gctcctccat ctcgagctat
     1141 ggcggcggcg gggggcgccg aggggcggcg cgcggccctg gaggcggcgg cggcggcagc
     1201 tcctgagcgg ggaggcggga gctgcgtgct gtgctgcgga gacctggagg ccacggcgct
     1261 gggccgctgc gaccacccgg tgtgctaccg ctgctctacc aagatgcggg tgctctgcga
     1321 gcagcgctac tgcgccgtgt gccgcgagga gctgcgccag gtggtctttg ggaagaagct
     1381 tcctgccttt gccaccatcc ccatccacca gctgcagcat gagaagaaat atgatatcta
     1441 ctttgcagat ggaaaggtgt acgcattgta caggcagctg ctgcagcacg agtgcccgcg
     1501 gtgccccgag ctgccacctt tcagcctctt cggggacctg gagcagcaca tgcggaggca
     1561 gcatgagctc ttctgctgcc gtctgtgcct ccagcacctc cagatcttca catatgagcg
     1621 caagtggtac tcgcgcaagg acctggcccg gcatcgcatg cagggtgacc ccgatgacac
     1681 gtcgcaccgt gggcacccgc tctgcaagtt ctgtgacgag cgctacctgg acaatgatga
     1741 gctgcttaag cacctgcgcc gcgaccacta cttctgccac ttctgcgact cggacggggc
     1801 ccaggactac tacagcgact atgcctacct gcgtgagcac ttccgggaga agcactttct
     1861 gtgtgaggaa ggccgctgca gcacggagca gttcacccac gccttccgca ccgagatcga
     1921 cctcaaggcc cacaggacgg cctgccacag tcgcagccgc gccgaggcac gccagaaccg
     1981 ccacatcgac ctgcagttca gctacgcgcc acggcactcg cgccggaacg agggggtcgt
     2041 tggtggcgaa gactacgagg aggtggacag gtacagccgc cagggccgag tggcccgggc
     2101 tggcactcgc ggagcccagc agagccgccg aggaagctgg aggtacaaaa gggaagaaga
     2161 ggaccgagaa gtagcagctg ctgtccgggc ctccgtggcc gcacagcagc aggaggaggc
     2221 ccgaagaagc gaagaccagg aggaaggcgg taggccaaaa aaggaagaag cggcggcgcg
     2281 gggacctgag gatccccgtg gcccccggcg ctcaccccgg actcagggcg aaggcccagg
     2341 ccccaaggaa acctcgacaa atggtcctgt aagccaagaa gccttctcgg tgacaggccc
     2401 agccgcccca gggtgtgtgg gggtgccagg cgccctccca ccacccagcc cgaagctcaa
     2461 ggacgaagac ttccccagcc tctctgcctc cacttcctcc tcctgctcca ctgcagcaac
     2521 cccgggccct gtggggttgg cgctgccgta cgccatccct gccagaggca ggagtgcctt
     2581 ccaggaggag gacttccccg ccctggtgtc ctcggtgccc aagcctggca ccgcccccac
     2641 cagccttgtc tctgcctgga acagcagcag tagcagcaag aaggtagcac agcccccact
     2701 ctcggcgcag gctaccggca gcggccagcc caccaggaag gctgggaagg ggagcagggg
     2761 cggcaggaag ggcggcccgc ccttcacaca ggaggaggag gaggacggcg gcccggccct
     2821 gcaggagctt ctgagcacac gccccacggg ctccgtctcc tccacactgg ggctggcctc
     2881 catccaaccc tctaaagttg ggaagaagaa gaaagtgggc tcggagaagc caggcaccac
     2941 attgccacag cccccgcccg ctacctgtcc cccaggggct ttgcaggccc cggaagctcc
     3001 tgccagcaga gccgaggggc cagttgccgt cgtcgttaat ggacacacgg agggcccggc
     3061 ccctgctcgg agtgccccca aggaaccccc tgggctccca aggcccctgg ggtccttccc
     3121 ctgccccacg ccacaggagg acttcccagc gctcggcggc ccctgcccac cccggatgcc
     3181 gccgccccca ggcttcagcg ctgtggtgct cctgaagggc acgcctcccc cacccccgcc
     3241 gggcctggtg cccccaatca gcaagccgcc ccctggcttc tctggccttc tgcctagccc
     3301 ccacccggcc tgtgtcccca gccccgccac caccaccacc acaaaagcac ccaggctgct
     3361 gcctgcccca cgggcgtacc tagtccccga gaacttccgg gagaggaacc ttcagctcat
     3421 ccagtccatc agggacttcc tgcagagcga cgaggcccgc ttcagcgagt tcaagagcca
     3481 ctcaggggag ttcagacagg gcctgatctc cgcagcccag tattacaaga gttgccggga
     3541 cctgctgggg gagaatttcc agaaggtctt taatgagctg ctggtcctgc tgcccgacac
     3601 ggccaagcag caggagctcc tgtctgcaca cacggacttc tgcaaccgcg agaagcctct
     3661 gagcaccaag tccaagaaga acaagaagag cgcgtggcag gccaccaccc agcaggcggg
     3721 cctggactgc cgtgtgtgcc ccacctgcca gcaggtgctc gcgcatggcg atgccagcag
     3781 ccaccaggcg ctgcatgctg cccgggacga cgacttcccc tccctgcaag ccatcgccag
     3841 gatcatcacg taggaattct gcagtcgacg gtaccgcggg cccgggatcc accggatcta
     3901 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     3961 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     4021 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4081 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg
     4141 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     4201 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     4261 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     4321 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     4381 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     4441 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     4501 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     4561 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     4621 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4681 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa
     4741 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     4801 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     4861 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     4921 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4981 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     5041 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag
     5101 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     5161 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     5221 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     5281 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
     5341 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     5401 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     5461 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     5521 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     5581 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     5641 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     5701 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     5761 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     5821 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     5881 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     5941 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     6001 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     6061 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
     6121 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
     6181 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     6241 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     6301 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     6361 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6421 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6481 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6541 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6601 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6661 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6721 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6781 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6841 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6901 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6961 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7021 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7081 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7141 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7201 tggataaccg tattaccgcc atgcat
//
