LOCUS       sequence-291323-        6632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsZNF598-del611-714-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6632)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-V5-SBP-C1-HsZNF598-del611-714-siRNAres_AH
FEATURES             Location/Qualifiers
     source          1..6632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             668..709
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             731..844
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWGGGHVVEGLAGELEQLRARLEHHPQGQREP"
     polyA_signal    3420..3541
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3457..3476)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3511..3530
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3548..4003)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3685..3706)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3897..3916
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4030..4134
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4136..4493
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4136..4327
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4159..4179)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4344..4479
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4528..5322
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4528..5322
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4582..4601)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5192..5211
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5510..5529)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5554..5601
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5930..6518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6419..6438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatgggt aagcctatcc ctaaccctct cctcggtctc gattctacgc ccggaagcgg
      721 tagcggtacg atggacgaga agaccaccgg ctgggggggc ggccacgtgg tggagggcct
      781 ggccggcgag ctggagcagc tgcgggccag gctggagcac caccctcagg gccagcggga
      841 gcccagatct cgagctatgg cggcggcggg gggcgccgag gggcggcgcg cggccctgga
      901 ggcggcggcg gcggcagctc ctgagcgggg aggcgggagc tgcgtgctgt gctgcggaga
      961 cctggaggcc acggcgctgg gccgctgcga ccacccggtg tgctaccgct gctctaccaa
     1021 gatgcgggtg ctctgcgagc agcgctactg cgccgtgtgc cgcgaggagc tgcgccaggt
     1081 ggtctttggg aagaagcttc ctgcctttgc caccatcccc atccaccagc tgcagcatga
     1141 gaagaaatat gatatctact ttgcagatgg aaaggtgtac gcattgtaca ggcagctgct
     1201 gcagcacgag tgcccgcggt gccccgagct gccacctttc agcctcttcg gggacctgga
     1261 gcagcacatg cggaggcagc atgagctctt ctgctgccgt ctgtgcctcc agcacctcca
     1321 gatcttcaca tatgagcgca agtggtactc gcgcaaggac ctggcccggc atcgcatgca
     1381 gggtgacccc gatgacacgt cgcaccgtgg gcacccgctc tgcaagttct gtgacgagcg
     1441 ctacctggac aatgatgagc tgcttaagca cctgcgccgc gaccactact tctgccactt
     1501 ctgcgactcg gacggggccc aggactacta cagcgactat gcctacctgc gtgagcactt
     1561 ccgggagaag cactttctgt gtgaggaagg ccgctgcagc acggagcagt tcacccacgc
     1621 cttccgcacc gagatcgacc tcaaggccca caggacggcc tgccacagtc gcagccgcgc
     1681 cgaggcacgc cagaaccgcc acatcgacct gcagttcagc tacgcgccac ggcactcgcg
     1741 ccggaacgag ggggtcgttg gtggcgaaga ctacgaggag gtggacaggt acagccgcca
     1801 gggccgagtg gcccgggctg gcactcgcgg agcccagcag agccgccgag gaagctggag
     1861 gtacaaaagg gaagaagagg accgagaagt agcagctgct gtccgggcct ccgtggccgc
     1921 acagcagcag gaggaggccc gaagaagcga agaccaggag gaaggcggta ggccaaaaaa
     1981 ggaagaagcg gcggcgcggg gacctgagga tccccgtggc ccccggcgct caccccggac
     2041 tcagggcgaa ggcccaggcc ccaaggaaac ctcgacaaat ggtcctgtaa gccaagaagc
     2101 cttctcggtg acaggcccag ccgccccagg gtgtgtgggg gtgccaggcg ccctcccacc
     2161 acccagcccg aagctcaagg acgaagactt ccccagcctc tctgcctcca cttcctcctc
     2221 ctgctccact gcagcaaccc cgggccctgt ggggttggcg ctgccgtacg ccatccctgc
     2281 cagaggcagg agtgccttcc aggaggagga cttccccgcc ctggtgtcct cggtgcccaa
     2341 gcctggcacc gcccccacca gccttgtctc tgcctggaac agcagcagta gcagcaagaa
     2401 ggtagcacag cccccactct cggcgcaggc taccggcagc ggccagccca ccaggaaggc
     2461 tgggaagggg agcaggggcg gcaggaaggg cggcccgccc ttcacacagg aggaggagga
     2521 ggacggcggc ccggccctgc aggagcttct gagcacacgc cccacgggct ccgtctcctc
     2581 cacactgggg ctggcctcca tccaaccctc taaagttggg aagaagaaga aagtgggctc
     2641 ggagaagcca ggcaccacat tgccacagcc cccgcccgct acctgttctg gccttctgcc
     2701 tagcccccac ccggcctgtg tccccagccc cgccaccacc accaccacaa aagcacccag
     2761 gctgctgcct gccccacggg cgtacctagt ccccgagaac ttccgggaga ggaaccttca
     2821 gctcatccag tccatcaggg acttcctgca gagcgacgag gcccgcttca gcgagttcaa
     2881 gagccactca ggggagttca gacagggcct gatctccgca gcccagtatt acaagagttg
     2941 ccgggacctg ctgggggaga atttccagaa ggtctttaat gagctgctgg tcctgctgcc
     3001 cgacacggcc aagcagcagg agctcctgtc tgcacacacg gacttctgca accgcgagaa
     3061 gcctctgagc accaagtcca agaagaacaa gaagagcgcg tggcaggcca ccacccagca
     3121 ggcgggcctg gactgccgtg tgtgccccac ctgccagcag gtgctcgcgc atggcgatgc
     3181 cagcagccac caggcgctgc atgctgcccg ggacgacgac ttcccctccc tgcaagccat
     3241 cgccaggatc atcacgtagg aattctgcag tcgacggtac cgcgggcccg ggatccaccg
     3301 gatctagata actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     3361 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     3421 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3481 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3541 aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     3601 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     3661 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     3721 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     3781 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     3841 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     3901 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     3961 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
     4021 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4081 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag
     4141 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     4201 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     4261 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4321 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     4381 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     4441 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat
     4501 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     4561 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     4621 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     4681 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc
     4741 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     4801 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     4861 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     4921 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     4981 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     5041 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     5101 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     5161 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     5221 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     5281 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     5341 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     5401 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     5461 gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga
     5521 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     5581 acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     5641 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     5701 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     5761 ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5821 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5881 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5941 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6001 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6061 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6121 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6181 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6241 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6301 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6361 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6421 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6481 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6541 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6601 attctgtgga taaccgtatt accgccatgc at
//
