LOCUS       sequence-291324-        6182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsZNF598-del611-864.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6182)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-V5-SBP-C1-HsZNF598-del611-864_AH
FEATURES             Location/Qualifiers
     source          1..6182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             668..709
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             731..844
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWGGGHVVEGLAGELEQLRARLEHHPQGQREP"
     polyA_signal    2970..3091
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3007..3026)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3061..3080
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3098..3553)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3235..3256)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3447..3466
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3580..3684
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3686..4043
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3686..3877
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3709..3729)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3894..4029
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4078..4872
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4078..4872
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4132..4151)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4742..4761
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5060..5079)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5104..5151
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5480..6068
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5969..5988
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatgggt aagcctatcc ctaaccctct cctcggtctc gattctacgc ccggaagcgg
      721 tagcggtacg atggacgaga agaccaccgg ctgggggggc ggccacgtgg tggagggcct
      781 ggccggcgag ctggagcagc tgcgggccag gctggagcac caccctcagg gccagcggga
      841 gcccagatct cgagctatgg cggcggcggg gggcgccgag gggcggcgcg cggccctgga
      901 ggcggcggcg gcggcagctc ctgagcgggg aggcgggagc tgcgtgctgt gctgcggaga
      961 cctggaggcc acggcgctgg gccgctgcga ccacccggtg tgctaccgct gctctaccaa
     1021 gatgcgggtg ctctgcgagc agcgctactg cgccgtgtgc cgcgaggagc tgcgccaggt
     1081 ggtctttggg aagaagcttc ctgcctttgc caccatcccc atccaccagc tgcagcatga
     1141 gaagaaatat gatatctact ttgcagatgg aaaggtgtac gcattgtaca ggcagctgct
     1201 gcagcacgag tgcccgcggt gccccgagct gccacctttc agcctcttcg gggacctgga
     1261 gcagcacatg cggaggcagc atgagctctt ctgctgccgt ctgtgcctcc agcacctcca
     1321 gatcttcaca tatgagcgca agtggtactc gcgcaaggac ctggcccggc atcgcatgca
     1381 gggtgacccc gatgacacgt cgcaccgtgg gcacccgctc tgcaagttct gtgacgagcg
     1441 ctacctggac aatgatgagc tgcttaagca cctgcgccgc gaccactact tctgccactt
     1501 ctgcgactcg gacggggccc aggactacta cagcgactat gcctacctgc gtgagcactt
     1561 ccgggagaag cactttctgt gtgaggaagg ccgctgcagc acggagcagt tcacccacgc
     1621 cttccgcacc gagatcgacc tcaaggccca caggacggcc tgccacagtc gcagccgcgc
     1681 cgaggcacgc cagaaccgcc acatcgacct gcagttcagc tacgcgccac ggcactcgcg
     1741 ccggaacgag ggggtcgttg gtggcgaaga ctacgaggag gtggacaggt acagccgcca
     1801 gggccgagtg gcccgggctg gcactcgcgg agcccagcag agccgccgag gaagctggag
     1861 gtacaaaagg gaagaagagg accgagaagt agcagctgct gtccgggcct ccgtggccgc
     1921 acagcagcag gaggaggccc gaagaagcga agaccaggag gaaggcggta ggccaaaaaa
     1981 ggaagaagcg gcggcgcggg gacctgagga tccccgtggc ccccggcgct caccccggac
     2041 tcagggcgaa ggcccaggcc ccaaggaaac ctcgacaaat ggtcctgtaa gccaagaagc
     2101 cttctcggtg acaggcccag ccgccccagg gtgtgtgggg gtgccaggcg ccctcccacc
     2161 acccagcccg aagctcaagg acgaagactt ccccagcctc tctgcctcca cttcctcctc
     2221 ctgctccact gcagcaaccc cgggccctgt ggggttggcg ctgccgtacg ccatccctgc
     2281 cagaggcagg agtgccttcc aggaggagga cttccccgcc ctggtgtcct cggtgcccaa
     2341 gcctggcacc gcccccacca gccttgtctc tgcctggaac agcagcagta gcagcaagaa
     2401 ggtagcacag cccccactct cggcgcaggc taccggcagc ggccagccca ccaggaaggc
     2461 tgggaagggg agcaggggcg gcaggaaggg cggcccgccc ttcacacagg aggaggagga
     2521 ggacggcggc ccggccctgc aggagcttct gagcacacgc cccacgggct ccgtctcctc
     2581 cacactgggg ctggcctcca tccaaccctc taaagttggg aagaagaaga aagtgggctc
     2641 ggagaagcca ggcaccacat tgccacagcc cccgcccgct acctgtcgtg tgtgccccac
     2701 ctgccagcag gtgctcgcgc atggcgatgc cagcagccac caggcgctgc atgctgcccg
     2761 ggacgacgac ttcccctccc tgcaagccat cgccaggatc atcacgtagg aattctgcag
     2821 tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata
     2881 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     2941 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     3001 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3061 gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg
     3121 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     3181 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     3241 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     3301 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     3361 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     3421 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     3481 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     3541 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3601 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3661 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     3721 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3781 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     3841 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     3901 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     3961 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     4021 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
     4081 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     4141 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     4201 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     4261 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     4321 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     4381 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     4441 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     4501 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     4561 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
     4621 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     4681 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     4741 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     4801 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     4861 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     4921 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     4981 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     5041 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     5101 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
     5161 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     5221 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     5281 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
     5341 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5401 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5461 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5521 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5581 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     5641 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5701 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5761 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5821 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5881 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5941 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6001 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6061 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6121 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc
     6181 at
//
