LOCUS       sequence-291329-       12363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmHelz.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12363)
  AUTHORS   Hanet A, Rasch F, Weber R, Ruscica V, Fauser M, Raisch T, 
            Kuzuoglu-Ozturk D, Chang CT, Bhandari D, Igreja C, Wohlbold L
  TITLE     HELZ directly interacts with CCR4-NOT and causes decay of bound 
            mRNAs.
  JOURNAL   Life Sci Alliance. 2019 Sep 30;2(5). pii: 2/5/e201900405. doi: 
            10.26508/lsa.201900405. Print 2019 Oct.
  PUBMED    31570513
REFERENCE   2  (bases 1 to 12363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2019-09-30"; volume: "2(5). pii: 2/5/e201900405. 
            doi"; pages: " 10.26508/lsa.201900405. Print 2019 Oct"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmHelz_AH
FEATURES             Location/Qualifiers
     source          1..12363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             9648..9689
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             9699..9716
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9739..9756)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9789..9923
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9813..9832)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9867..9886
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(10545..11133)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10625..10644)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11304..12095,12096..12164))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11927..11946
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12165..12269)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12337..12355
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgatggccg ccgagaagga gatgcaggcc cacgacatgg tccgacgacg
     3361 gagttgggcc cgtgctgtgg cgctctacga ccagctgatt gcgcccagtc ccggccaggg
     3421 aagcggtggc cagagtgcag gatcaagggc ccagaaggac cagcagatcg cctgcctact
     3481 gggacgatgc gagtgcctcc tagagctggg aaagttcgag ggttgtctgg cggacgccta
     3541 caaggtgctc acgctgctct ccgagcaaac agagtgcctg gccagcgtgt ccagggcacg
     3601 ccgatggctc gtccacgctc ttttcaagat gcacaagtac ggggatgccg aaatcttcct
     3661 gagcaagtgg atcaacgagc tgagcggcca gcagatatac tcggacatat cgaaaaccct
     3721 tgagcggtac aagtccatca ttcagatgat catgaacggg cagcacaagg cgcaggccct
     3781 gaagatccca cacgcccgtc tcgaggacga gatggctata ctggatacca aactggatca
     3841 ctgggccacc aacaacctgc cgctggacaa gtacagcaag ctgctcagca acaagggctt
     3901 gaagaagcgg gagcagcagc agcaacagca gcagcaacaa cagcagcaga atggcaatgg
     3961 aatcggcagt tatgcgagca gcagcagtag ctcctcaact ggcagcagca gcacgatgtc
     4021 cagttccagc acaagtctcc acaagaccaa cgatctgctg gcggccatga agctcacggc
     4081 aggaaaacac cagccgggct ccggcgacgg atctggctcg gatcagggcg atcaggcggc
     4141 tccatcgacc acctgcagct actgcaccat cacctttcag acgaggaacg aactgcgaca
     4201 gcactgccag accgaggcgc accagaaggt gatcatgagc gatgagggtc gtgactggaa
     4261 gtggcgtccg ccgccccgtg gttatacact ggacacctat tccctgtgcg agacgttcag
     4321 cgaggcgcac atctgtcatt atggagccca gtgcgtggag gcccatggac aggatgagtt
     4381 gaacgagtgg aaggagcgct tcgagtaccg gcgaatgcgg atgcagaagg cttgtgagaa
     4441 ggagctctac ggcaagtcgt acacggagca agtgctggat cgttggattc aggcgacagc
     4501 accggagcgg gtgatgtgcg agcgagtgcc ggacatggag gcacgctgtg agcagcagct
     4561 ggtgactagc attagctcaa agacctccaa gcgggagtgg ctcttccaat tagagacgaa
     4621 gaagccgcta aaagcggtag ccctcttgca ggatgcccat cgaaatcact ttgccctgaa
     4681 gtccataaaa atgtgcaagc caacggagtc cagcatggaa ctcaaatcag accaagagtg
     4741 gctggcaggc agttcatcgc aatcggagac ttccaacgag gactctgcta ctttgattca
     4801 cgaaatcaca gttgagtttc acacggagat ctacggcacc tttcggcaag ccatctgctt
     4861 cgatgtgggt caggagcctt tgcttgtgag gcatctgtgc gtggatgtgt tgcccgtgaa
     4921 cgatgcggag aaaatcgagg agatcaagcg ggacatcatt aacagttctg ccacccgatg
     4981 ggatgtcgcc aatacgcagc tcacacgctt cgaaacgacg attggtactc atctcaaaac
     5041 ggaagcgtat ctcagcgatt tggaacacga aagggagcta ttggagaggt atccctgtcc
     5101 cagggcagcc actttcactc tgacccagtc gaccattgtg gagaagcggt tgacgcagaa
     5161 taactaccgc tcacgcatcc acgagttgtt gtcagtggag gagattgccc gctatgagca
     5221 gatagcgagg tataatgtaa ggactcgtct cacagtggca tcgaattaca ttcttacccc
     5281 cgcgggaatg gccaccagta cggccaagta ttccctggca ggtgaactgt ttgctcttat
     5341 gcgtttgggc aaggacattt cggaggatac gtctcctggc agattaattc tctcgaactg
     5401 cagcagtgtg tatatctcaa aacctgagga tcccgattcg aaagcagacc caactaaacg
     5461 tgcagtttac gaagcgttga tcgaggacaa aggcaagaat gtgatttact tgaaactatc
     5521 cgcaaaatgt gtggaggcaa tggctctgca ggcggatacc gaactggagg tggacataca
     5581 attccagctg aaccgaatgc catactgtga gtggcacaat gcggtggata agatcacaga
     5641 tttccggttg atctttccgg ctacggaact ggaaccgagt ataccgtgga caccgaagaa
     5701 gcagtgggcc gattcctgtg aaccgaagtt gaatgccaaa caaagggagg ccgtgaatgc
     5761 cattaccaca gctctgagca ttaagttgcc gccaatcctg ctgataggac cctttggcac
     5821 tggaaaaacc tacacgctgg cccaagctat caaacaactg ctcacgcagc cggaggccaa
     5881 aattctgatc tgcacgcact cgaattcagc cgctgacttg tacatcaagg aatacctgca
     5941 tccgtggatc gaggagggtt tgaaggaggc cacaccgttg cgagtttact accataagag
     6001 atggtcagcc acggtaaacg gtgtggtgca aaaatactgc atcaccgatg gagttggcaa
     6061 cttcctaagg cccactgtgg aggatataat gcgtcataga attgtagttg ttacgctgag
     6121 tatcagcatg gagttggcca ctttgggtct gcccaaggga ttgttcactc acatctttct
     6181 ggacgaagct gctcaggcaa tggaatgcga ggccattatg ccgcttgctt tggccaatga
     6241 ttctacaagg atcgttttgg ctggagatca catgcaaatg agtccggaac tgttctcagc
     6301 ctttgccaag gaacgcaagc tgcacatttc gctgttggag cggctgtacg accactatcc
     6361 ctccaacttc ccctgcaaga tcctgctgtg cgagaactac cgtgcccacg aggccatcat
     6421 ccgtttcact tcggagcttt tctacgaaca aaagctggtg gcctccggaa aacagccacg
     6481 gcacgatcgc ttttatccct tgaccttctt cactactcgg ggagaggatg ttcaggataa
     6541 aaactcaact gctttctata ataatgctga agtttatgag gtggtggaaa gggtttccga
     6601 gctgcggaaa cgttggccca gtgcttgggg taaactgaat gataccagca ttggcattat
     6661 gactccctac tcggatcagg tgtttcgcat tcgttccgag ctgagaaaac ggcgcatggg
     6721 tggaatatcc gtagaacgtg tgctcaatgt ccagggtaaa cagtttcggg cggtattcct
     6781 ctctacagtt cgaacccgac gcacctgcat gccccaagga agtgcccctg gttccgcttc
     6841 cacaaccagc attggcgatg ccgacgcaga ctatggattc ttgagcaatt ccaagctgct
     6901 gaacacggct attactcgtg cccagtcgtt ggttgcagtg gttggtgatc ccgtggctct
     6961 ttgttccatt ggtcgctgcc ggaaagtgtg ggagcgtttc atcgaaatct gcgacgaaca
     7021 taaaagtctt tttggcttaa cttggtcgaa cctgcgctcc caactggatg gcgtggagct
     7081 taagcgtggc tatgtgctaa atcctctggc tcccgagttc gtcccgcgtg cccttcaacc
     7141 ggaggcatat ctgcgggagc aggctgctct ctatctaaat ggtccgcaac acggacacgg
     7201 cggccatgga ccgccgggtc ctccggctca ttttgccaac gcagctggaa tgctgggtcc
     7261 cggacccgcg gccgctgctc accagatgaa tcaaatggct gctgccatgg ctgccaatca
     7321 gcaacagctc acagcccaca tgtactccac ccacatggcc atgatggccg ctgccgtcgg
     7381 aggaaagtct ggaaatggcc ctggaccagg aaccggcgga ggacccggtc ctagtccagg
     7441 cccacctcca cactacggcg gcggcggtgg tggtggcgga cacatgggaa tgagaggacc
     7501 accgccaccg gcgccgcatc tgcgtggaat gccacccggt ggaccgccac ccacacagca
     7561 gccaggcgga gctggtggcg gcgggggtcc accgccacca tacggacaat atccagccat
     7621 gcagcactgg cgaccaccgc agcagggaca gaatcaaggc ccaggacagc agccaccaca
     7681 aaatccgaat gccagtctct ggggtccgcc gccgcaaacg aatccgtgga gtgtgctgcc
     7741 gcccagtaag cagccacagc aacctcagcc accgccagtt agcgcgcccg gacgtggacc
     7801 gggtcctttg ccaccgccac tcaatgcaaa cccgtctctt ccactgagag gtggtagtgt
     7861 gccgccaccg ccgccgaatg cctcgactgc tgctcacctg ctaaggaatc ccagcgaact
     7921 gggctacttg gccaagaagt cgctgtacaa ccatggccag gcattgccgc atcatcagct
     7981 ttatggaccc ggaccaggac caccgccgcc tccatcgcag caacagccgc aggcaccgcc
     8041 gatgggtatg cagaggggca gcagtgtgcc gaacggaccc atgtcgccca tggagaacgg
     8101 accgccgcca cctccctatc tgaggcacaa tggtagtggc tacaatccgg gagcaccgcc
     8161 accgcctccg cagcaacaac cacagccacc acccaatccg tttatgtacg ccggcaagca
     8221 accgtctccg agctctatga gatttggacc aatgacgcac gagctactcc cacccaatgt
     8281 aagcatctat gacatggctt ttaatccgaa agacgagcaa tacaagtggt acgttaaact
     8341 gcttgaaacc gtgggtcagg atggtgccaa caagtttgcg gaaatgctgc gccaggtgat
     8401 ggccacaatg caacagcagc agcagcaaca taagccaccg cagccaccga tggctaacaa
     8461 tccgatgctc aataatccgc acgtgcagcg accacagtat cccatgatgt atccacaggg
     8521 tcaacagcaa cagcagcagc agcccccaca gcaatctgta gatgcttcac cgccgctgga
     8581 gaacttcaac cagcagatcg atcttgtttt caattcgatt atgaatggag ccaatgatgt
     8641 tgcgcagccc atactgcgcg atgtcctggg catggtagct ggcacgggca atgctcctgg
     8701 tcctccgctt agcaacggtt tgaacggagt cgatctccag cagcagcagc agcaacaaca
     8761 gcagcagcag caaagccgcc tattcgcaat gatgcagcaa caacaacagc agcagcaaca
     8821 acaacaggcc aagccaccgc taggacctgg accactttat cccaatcatc tgggcggacc
     8881 tggtcttcac caggcgccgc ccaatggtgg aggtaatctg agcggcgtcg gaaacaccaa
     8941 ctttatgggc aacgccggaa atgctctgct caatgacaag ccctacaaca acgtgggcat
     9001 gcacaacaac gtgggcttga acatcggtgg cgcaggagtt gctggtcaca atagtaataa
     9061 caacaatgga atgctgacca atggcaacaa ctcggtgccc ttgtatcgcc gccaggcgca
     9121 caccggaaac gggattggag gcggaaatgg tgactacaac aacatgcatg gaatggatgc
     9181 tggcgccaac ttgatagagg cactcttcca ggcaaacaac tcggtggtgg atcactcggt
     9241 gaagtcgtcg gaccatatgc acggcctgct gtacaacaat ttcaatgggc tgaagggcgg
     9301 cggccacgaa ctggacctgt tccaggcgat ggcgcccagt gtgccgcact cggaggcagc
     9361 taatcatcac cagcagtcct ctggctcatc ggcaaccacc tatgcggctg tgctgagcca
     9421 gggaccacag gcggtggttg gcggaggcgt tggaacagga ccatcgccgg gacagtcgca
     9481 ggcgcaggcg ggcgtggctg gttcaggaac gggcggaaaa gtcgcgccgg cggttggtgg
     9541 taacgatctt ctggagaagg atccgtttgc ggccatccgg gagttgggac aggccacgac
     9601 cggattctac aactattttc agtaatctag agggcccgcg gttcgaaggt aagcctatcc
     9661 ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag
     9721 tttaaacccg ctgatcagcc tcgactgtgc cttctaaggc ctgagctcgc tgatcagcct
     9781 cgatcgagga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     9841 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     9901 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     9961 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
    10021 attatgatca gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
    10081 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
    10141 ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg
    10201 acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc
    10261 gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg
    10321 gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc
    10381 attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc
    10441 aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc
    10501 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    10561 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    10621 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    10681 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
    10741 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    10801 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    10861 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    10921 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    10981 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    11041 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    11101 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    11161 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    11221 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    11281 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    11341 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    11401 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    11461 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    11521 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    11581 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
    11641 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    11701 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    11761 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    11821 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    11881 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
    11941 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    12001 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    12061 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    12121 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    12181 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    12241 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    12301 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    12361 cgt
//
