LOCUS       sequence-291334-        9093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmHelz_1-1212.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Hanet A, Rasch F, Weber R, Ruscica V, Fauser M, Raisch T, 
            Kuzuoglu-Ozturk D, Chang CT, Bhandari D, Igreja C, Wohlbold L
  TITLE     HELZ directly interacts with CCR4-NOT and causes decay of bound 
            mRNAs.
  JOURNAL   Life Sci Alliance. 2019 Sep 30;2(5). pii: 2/5/e201900405. doi: 
            10.26508/lsa.201900405. Print 2019 Oct.
  PUBMED    31570513
REFERENCE   2  (bases 1 to 9093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2019-09-30"; volume: "2(5). pii: 2/5/e201900405. 
            doi"; pages: " 10.26508/lsa.201900405. Print 2019 Oct"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmHelz_1-1212_AH
FEATURES             Location/Qualifiers
     source          1..9093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6378..6419
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6429..6446
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6469..6486)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6519..6653
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6543..6562)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6597..6616
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7275..7863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7355..7374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8034..8825,8826..8894))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8657..8676
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8895..8999)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9067..9085
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgatgg ccgccgagaa ggagatgcag gcccacgaca tggtccgacg
     2761 acggagttgg gcccgtgctg tggcgctcta cgaccagctg attgcgccca gtcccggcca
     2821 gggaagcggt ggccagagtg caggatcaag ggcccagaag gaccagcaga tcgcctgcct
     2881 actgggacga tgcgagtgcc tcctagagct gggaaagttc gagggttgtc tggcggacgc
     2941 ctacaaggtg ctcacgctgc tctccgagca aacagagtgc ctggccagcg tgtccagggc
     3001 acgccgatgg ctcgtccacg ctcttttcaa gatgcacaag tacggggatg ccgaaatctt
     3061 cctgagcaag tggatcaacg agctgagcgg ccagcagata tactcggaca tatcgaaaac
     3121 ccttgagcgg tacaagtcca tcattcagat gatcatgaac gggcagcaca aggcgcaggc
     3181 cctgaagatc ccacacgccc gtctcgagga cgagatggct atactggata ccaaactgga
     3241 tcactgggcc accaacaacc tgccgctgga caagtacagc aagctgctca gcaacaaggg
     3301 cttgaagaag cgggagcagc agcagcaaca gcagcagcaa caacagcagc agaatggcaa
     3361 tggaatcggc agttatgcga gcagcagcag tagctcctca actggcagca gcagcacgat
     3421 gtccagttcc agcacaagtc tccacaagac caacgatctg ctggcggcca tgaagctcac
     3481 ggcaggaaaa caccagccgg gctccggcga cggatctggc tcggatcagg gcgatcaggc
     3541 ggctccatcg accacctgca gctactgcac catcaccttt cagacgagga acgaactgcg
     3601 acagcactgc cagaccgagg cgcaccagaa ggtgatcatg agcgatgagg gtcgtgactg
     3661 gaagtggcgt ccgccgcccc gtggttatac actggacacc tattccctgt gcgagacgtt
     3721 cagcgaggcg cacatctgtc attatggagc ccagtgcgtg gaggcccatg gacaggatga
     3781 gttgaacgag tggaaggagc gcttcgagta ccggcgaatg cggatgcaga aggcttgtga
     3841 gaaggagctc tacggcaagt cgtacacgga gcaagtgctg gatcgttgga ttcaggcgac
     3901 agcaccggag cgggtgatgt gcgagcgagt gccggacatg gaggcacgct gtgagcagca
     3961 gctggtgact agcattagct caaagacctc caagcgggag tggctcttcc aattagagac
     4021 gaagaagccg ctaaaagcgg tagccctctt gcaggatgcc catcgaaatc actttgccct
     4081 gaagtccata aaaatgtgca agccaacgga gtccagcatg gaactcaaat cagaccaaga
     4141 gtggctggca ggcagttcat cgcaatcgga gacttccaac gaggactctg ctactttgat
     4201 tcacgaaatc acagttgagt ttcacacgga gatctacggc acctttcggc aagccatctg
     4261 cttcgatgtg ggtcaggagc ctttgcttgt gaggcatctg tgcgtggatg tgttgcccgt
     4321 gaacgatgcg gagaaaatcg aggagatcaa gcgggacatc attaacagtt ctgccacccg
     4381 atgggatgtc gccaatacgc agctcacacg cttcgaaacg acgattggta ctcatctcaa
     4441 aacggaagcg tatctcagcg atttggaaca cgaaagggag ctattggaga ggtatccctg
     4501 tcccagggca gccactttca ctctgaccca gtcgaccatt gtggagaagc ggttgacgca
     4561 gaataactac cgctcacgca tccacgagtt gttgtcagtg gaggagattg cccgctatga
     4621 gcagatagcg aggtataatg taaggactcg tctcacagtg gcatcgaatt acattcttac
     4681 ccccgcggga atggccacca gtacggccaa gtattccctg gcaggtgaac tgtttgctct
     4741 tatgcgtttg ggcaaggaca tttcggagga tacgtctcct ggcagattaa ttctctcgaa
     4801 ctgcagcagt gtgtatatct caaaacctga ggatcccgat tcgaaagcag acccaactaa
     4861 acgtgcagtt tacgaagcgt tgatcgagga caaaggcaag aatgtgattt acttgaaact
     4921 atccgcaaaa tgtgtggagg caatggctct gcaggcggat accgaactgg aggtggacat
     4981 acaattccag ctgaaccgaa tgccatactg tgagtggcac aatgcggtgg ataagatcac
     5041 agatttccgg ttgatctttc cggctacgga actggaaccg agtataccgt ggacaccgaa
     5101 gaagcagtgg gccgattcct gtgaaccgaa gttgaatgcc aaacaaaggg aggccgtgaa
     5161 tgccattacc acagctctga gcattaagtt gccgccaatc ctgctgatag gaccctttgg
     5221 cactggaaaa acctacacgc tggcccaagc tatcaaacaa ctgctcacgc agccggaggc
     5281 caaaattctg atctgcacgc actcgaattc agccgctgac ttgtacatca aggaatacct
     5341 gcatccgtgg atcgaggagg gtttgaagga ggccacaccg ttgcgagttt actaccataa
     5401 gagatggtca gccacggtaa acggtgtggt gcaaaaatac tgcatcaccg atggagttgg
     5461 caacttccta aggcccactg tggaggatat aatgcgtcat agaattgtag ttgttacgct
     5521 gagtatcagc atggagttgg ccactttggg tctgcccaag ggattgttca ctcacatctt
     5581 tctggacgaa gctgctcagg caatggaatg cgaggccatt atgccgcttg ctttggccaa
     5641 tgattctaca aggatcgttt tggctggaga tcacatgcaa atgagtccgg aactgttctc
     5701 agcctttgcc aaggaacgca agctgcacat ttcgctgttg gagcggctgt acgaccacta
     5761 tccctccaac ttcccctgca agatcctgct gtgcgagaac taccgtgccc acgaggccat
     5821 catccgtttc acttcggagc ttttctacga acaaaagctg gtggcctccg gaaaacagcc
     5881 acggcacgat cgcttttatc ccttgacctt cttcactact cggggagagg atgttcagga
     5941 taaaaactca actgctttct ataataatgc tgaagtttat gaggtggtgg aaagggtttc
     6001 cgagctgcgg aaacgttggc ccagtgcttg gggtaaactg aatgatacca gcattggcat
     6061 tatgactccc tactcggatc aggtgtttcg cattcgttcc gagctgagaa aacggcgcat
     6121 gggtggaata tccgtagaac gtgtgctcaa tgtccagggt aaacagtttc gggcggtatt
     6181 cctctctaca gttcgaaccc gacgcacctg catgccccaa ggaagtgccc ctggttccgc
     6241 ttccacaacc agcattggcg atgccgacgc agactatgga ttcttgagca attccaagct
     6301 gctgaacacg gctattactc gtgcccagtc gttggttgca gtggttggtg attaatctag
     6361 agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc
     6421 gtaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc
     6481 cttctaaggc ctgagctcgc tgatcagcct cgatcgagga tccagacatg ataagataca
     6541 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     6601 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     6661 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     6721 agtaaaacct ctacaaatgt ggtatggctg attatgatca gtcgaccgat gcccttgaga
     6781 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     6841 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
     6901 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     6961 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
     7021 gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg
     7081 ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc
     7141 gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag
     7201 cttcaaggat cgctcgcggc tcttaccagc cagcaaaagg ccaggaaccg taaaaaggcc
     7261 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7321 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7381 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7441 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     7501 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7561 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7621 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7681 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     7741 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7861 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7921 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7981 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8041 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8101 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8161 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8221 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8281 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8341 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8401 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8461 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8521 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8581 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8641 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8701 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8761 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8821 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8881 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8941 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9001 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     9061 tataaaaata ggcgtatcac gaggcccttt cgt
//
