LOCUS       sequence-291335-        8130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmHelz_1213-2103.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8130)
  AUTHORS   Hanet A, Rasch F, Weber R, Ruscica V, Fauser M, Raisch T, 
            Kuzuoglu-Ozturk D, Chang CT, Bhandari D, Igreja C, Wohlbold L
  TITLE     HELZ directly interacts with CCR4-NOT and causes decay of bound 
            mRNAs.
  JOURNAL   Life Sci Alliance. 2019 Sep 30;2(5). pii: 2/5/e201900405. doi: 
            10.26508/lsa.201900405. Print 2019 Oct.
  PUBMED    31570513
REFERENCE   2  (bases 1 to 8130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2019-09-30"; volume: "2(5). pii: 2/5/e201900405. 
            doi"; pages: " 10.26508/lsa.201900405. Print 2019 Oct"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmHelz_1213-2103_AH
FEATURES             Location/Qualifiers
     source          1..8130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5415..5456
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5466..5483
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5506..5523)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5556..5690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5580..5599)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5634..5653
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6312..6900)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6392..6411)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7071..7862,7863..7931))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7694..7713
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7932..8036)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8104..8122
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttccccg tggctctttg ttccattggt cgctgccgga aagtgtggga
     2761 gcgtttcatc gaaatctgcg acgaacataa aagtcttttt ggcttaactt ggtcgaacct
     2821 gcgctcccaa ctggatggcg tggagcttaa gcgtggctat gtgctaaatc ctctggctcc
     2881 cgagttcgtc ccgcgtgccc ttcaaccgga ggcatatctg cgggagcagg ctgctctcta
     2941 tctaaatggt ccgcaacacg gacacggcgg ccatggaccg ccgggtcctc cggctcattt
     3001 tgccaacgca gctggaatgc tgggtcccgg acccgcggcc gctgctcacc agatgaatca
     3061 aatggctgct gccatggctg ccaatcagca acagctcaca gcccacatgt actccaccca
     3121 catggccatg atggccgctg ccgtcggagg aaagtctgga aatggccctg gaccaggaac
     3181 cggcggagga cccggtccta gtccaggccc acctccacac tacggcggcg gcggtggtgg
     3241 tggcggacac atgggaatga gaggaccacc gccaccggcg ccgcatctgc gtggaatgcc
     3301 acccggtgga ccgccaccca cacagcagcc aggcggagct ggtggcggcg ggggtccacc
     3361 gccaccatac ggacaatatc cagccatgca gcactggcga ccaccgcagc agggacagaa
     3421 tcaaggccca ggacagcagc caccacaaaa tccgaatgcc agtctctggg gtccgccgcc
     3481 gcaaacgaat ccgtggagtg tgctgccgcc cagtaagcag ccacagcaac ctcagccacc
     3541 gccagttagc gcgcccggac gtggaccggg tcctttgcca ccgccactca atgcaaaccc
     3601 gtctcttcca ctgagaggtg gtagtgtgcc gccaccgccg ccgaatgcct cgactgctgc
     3661 tcacctgcta aggaatccca gcgaactggg ctacttggcc aagaagtcgc tgtacaacca
     3721 tggccaggca ttgccgcatc atcagcttta tggacccgga ccaggaccac cgccgcctcc
     3781 atcgcagcaa cagccgcagg caccgccgat gggtatgcag aggggcagca gtgtgccgaa
     3841 cggacccatg tcgcccatgg agaacggacc gccgccacct ccctatctga ggcacaatgg
     3901 tagtggctac aatccgggag caccgccacc gcctccgcag caacaaccac agccaccacc
     3961 caatccgttt atgtacgccg gcaagcaacc gtctccgagc tctatgagat ttggaccaat
     4021 gacgcacgag ctactcccac ccaatgtaag catctatgac atggctttta atccgaaaga
     4081 cgagcaatac aagtggtacg ttaaactgct tgaaaccgtg ggtcaggatg gtgccaacaa
     4141 gtttgcggaa atgctgcgcc aggtgatggc cacaatgcaa cagcagcagc agcaacataa
     4201 gccaccgcag ccaccgatgg ctaacaatcc gatgctcaat aatccgcacg tgcagcgacc
     4261 acagtatccc atgatgtatc cacagggtca acagcaacag cagcagcagc ccccacagca
     4321 atctgtagat gcttcaccgc cgctggagaa cttcaaccag cagatcgatc ttgttttcaa
     4381 ttcgattatg aatggagcca atgatgttgc gcagcccata ctgcgcgatg tcctgggcat
     4441 ggtagctggc acgggcaatg ctcctggtcc tccgcttagc aacggtttga acggagtcga
     4501 tctccagcag cagcagcagc aacaacagca gcagcagcaa agccgcctat tcgcaatgat
     4561 gcagcaacaa caacagcagc agcaacaaca acaggccaag ccaccgctag gacctggacc
     4621 actttatccc aatcatctgg gcggacctgg tcttcaccag gcgccgccca atggtggagg
     4681 taatctgagc ggcgtcggaa acaccaactt tatgggcaac gccggaaatg ctctgctcaa
     4741 tgacaagccc tacaacaacg tgggcatgca caacaacgtg ggcttgaaca tcggtggcgc
     4801 aggagttgct ggtcacaata gtaataacaa caatggaatg ctgaccaatg gcaacaactc
     4861 ggtgcccttg tatcgccgcc aggcgcacac cggaaacggg attggaggcg gaaatggtga
     4921 ctacaacaac atgcatggaa tggatgctgg cgccaacttg atagaggcac tcttccaggc
     4981 aaacaactcg gtggtggatc actcggtgaa gtcgtcggac catatgcacg gcctgctgta
     5041 caacaatttc aatgggctga agggcggcgg ccacgaactg gacctgttcc aggcgatggc
     5101 gcccagtgtg ccgcactcgg aggcagctaa tcatcaccag cagtcctctg gctcatcggc
     5161 aaccacctat gcggctgtgc tgagccaggg accacaggcg gtggttggcg gaggcgttgg
     5221 aacaggacca tcgccgggac agtcgcaggc gcaggcgggc gtggctggtt caggaacggg
     5281 cggaaaagtc gcgccggcgg ttggtggtaa cgatcttctg gagaaggatc cgtttgcggc
     5341 catccgggag ttgggacagg ccacgaccgg attctacaac tattttcagt aatctagagg
     5401 gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta
     5461 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
     5521 ctaaggcctg agctcgctga tcagcctcga tcgaggatcc agacatgata agatacattg
     5581 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     5641 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     5701 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     5761 aaaacctcta caaatgtggt atggctgatt atgatcagtc gaccgatgcc cttgagagcc
     5821 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
     5881 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc
     5941 ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga
     6001 atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag
     6061 aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc
     6121 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg
     6181 atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt
     6241 caaggatcgc tcgcggctct taccagccag caaaaggcca ggaaccgtaa aaaggccgcg
     6301 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6361 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6421 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6481 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     6541 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6601 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6661 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6721 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     6781 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6841 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6901 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6961 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7021 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7081 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7141 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7201 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7261 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7321 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7381 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7441 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7501 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7561 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7621 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7681 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7741 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7801 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7861 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7921 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7981 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8041 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     8101 aaaaataggc gtatcacgag gccctttcgt
//
