LOCUS       sequence-291346-        9193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsHelz_1-1474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9193)
  AUTHORS   Hanet A, Rasch F, Weber R, Ruscica V, Fauser M, Raisch T, 
            Kuzuoglu-Ozturk D, Chang CT, Bhandari D, Igreja C, Wohlbold L
  TITLE     HELZ directly interacts with CCR4-NOT and causes decay of bound 
            mRNAs.
  JOURNAL   Life Sci Alliance. 2019 Sep 30;2(5). pii: 2/5/e201900405. doi: 
            10.26508/lsa.201900405. Print 2019 Oct.
  PUBMED    31570513
REFERENCE   2  (bases 1 to 9193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2019-09-30"; volume: "2(5). pii: 2/5/e201900405. 
            doi"; pages: " 10.26508/lsa.201900405. Print 2019 Oct"
COMMENT     Sequence Label: pT7-EGFP-HsHelz_1-1474_AH
FEATURES             Location/Qualifiers
     source          1..9193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(710..731)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(971..990)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1318..1339
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5981..6102
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6018..6037)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6072..6091
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6109..6564)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6246..6267)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6458..6477
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6591..6695
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6697..7054
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6697..6888
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6720..6740)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6905..7040
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7089..7883
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7089..7883
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7143..7162)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7753..7772
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8071..8090)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8115..8162
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8491..9079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8980..8999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      721 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      781 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      841 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      901 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      961 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1021 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1081 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1141 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1201 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1261 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1321 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1381 gtccggactc agatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcggatat
     1441 ggaagacaga agagctgaaa agtcatgtga acaagcatgt gaatcactta agaggcagga
     1501 ctatgaaatg gccctcaagc actgcacaga ggcccttctt tctcttggcc agtactccat
     1561 ggcagacttc acagggcctt gtccattgga aatagaacgc atcaaaatcg agagtcttct
     1621 ctacagaatt gcctcatttt tgcaactgaa aaattatgtg caagctgatg aagattgtag
     1681 acatgtgctg ggagaaggac tggccaaggg agaagatgcc tttcgggcag tgctttgctg
     1741 catgcagctg aaagggaagc tccaacctgt atccaccatt cttgccaagt cactcacagg
     1801 agagtccctg aatgggatgg taacaaagga tttgacaaga ctaaaaacac ttctctcaga
     1861 aacagagaca gcaactagta acgccctctc tggatatcac gtggaagact tagatgaggg
     1921 gtcttgtaat ggttggcatt tccgcccacc acctagggga atcacaagca gcgaggaata
     1981 tactttgtgt aaaagatttt tagaacaagg aatctgtagg tatggtgccc agtgtacttc
     2041 agcacattcc caggaagaac tagcagaatg gcagaaaaga tatgcttcac ggctgataaa
     2101 attgaaacag caaaatgaga ataaacagct ctcaggcagt tacatggaaa ccttgataga
     2161 aaagtggatg aattcattgt ctcctgagaa agtgcttagt gaatgtatag aaggagtaaa
     2221 ggtagagcac aatcctgacc tgtcagttac tgtcagcacc aaaaaatccc accagacatg
     2281 gacctttgct ctcacttgta agcctgcaag aatgctgtat cgtgtagcat tgctttatga
     2341 tgctcatcgt cctcatttta gtatcattgc aatatctgcc ggagatagta ctacccaggt
     2401 atcacaagaa gtcccagaaa actgtcaaga atggatagga ggaaagatgg cccaaaatgg
     2461 attagatcat tacgtgtata aagtcgggat agcatttaac acagaaatat ttggaacttt
     2521 tcgccaaacc atagttttcg actttggatt ggaaccagta ctcatgcaaa gagtaatgat
     2581 tgatgcagct tctacagaag atctcgaata cctgatgcat gcaaaacagc agctagtaac
     2641 cacagctaaa cgttgggatt cttcctctaa gactattata gattttgaac ctaatgaaac
     2701 tactgatttg gagaagagcc ttcttatcag ataccaaatt cccctctctg ctgaccagct
     2761 atttactcag tccgttttag acaaatcatt gaccaagagc aactatcagt cacggttaca
     2821 tgaccttctt tatattgagg agatagccca gtataaagaa atcagcaagt tcaaccttaa
     2881 agtgcaattg cagattctgg caagcttcat gctcactggt gtttctggag gtgcaaagta
     2941 tgctcagaat ggacaacttt ttggtcgctt taagcttact gaaacacttt ctgaagatac
     3001 tttggctgga cgactggtga tgaccaaagt caatgctgtt tatttattac cagtccctaa
     3061 acagaagtta gtacagaccc agggaaccaa agagaaggtt tatgaagcta ctattgaaga
     3121 aaaaacaaag gaatatatat ttttaaggct atctagggaa tgctgtgaag aacttaatct
     3181 tcggcctgac tgtgacacac aggttgaact tcagtttcaa ttaaatcgat tacccctctg
     3241 tgaaatgcac tatgcactag acaggatcaa ggacaatggg gttttgtttc cagacatcag
     3301 tatgactccc accataccat ggagtcctaa cagacaatgg gatgaacagt tggatcctcg
     3361 actaaatgca aaacagaaag aggctgttct ggccattacc actccacttg caatccagct
     3421 gccgcctgtg cttatcatcg gaccctatgg gacaggcaaa acgttcactc tagctcaggc
     3481 tgtcaaacat attctgcagc aacaggagac taggattctc atttgcaccc attctaatag
     3541 tgctgctgat ctctacataa aggattattt acatccatat gtagaagcag gcaatcccca
     3601 ggcaagacct ctcagggtat atttcagaaa tcgctgggta aagactgtcc acccagttgt
     3661 gcatcagtac tgtttgatct caagcgcaca ttccaccttt cagatgcccc agaaagaaga
     3721 tattcttaaa catcgagtgg tggttgttac cttgaatact tcccagtacc tctgtcagtt
     3781 ggaccttgaa cctgggtttt ttacacacat tctattagat gaagctgccc aggccatgga
     3841 gtgtgaaacc attatgcctc tagcattagc aactcaaaac actcggattg tcttggctgg
     3901 tgatcacatg cagctcagtc cttttgttta cagcgagttt gccagggaga gaaaccttca
     3961 cgtttcatta cttgaccgac tctatgagca ttaccctgct gagttcccat gtaggattct
     4021 cctgtgtgag aactaccgct cccatgaagc tatcatcaat tatacctctg agcttttcta
     4081 tgagggcaaa ctgatggcca gtgggaagca gccagcacac aaagatttct acccactaac
     4141 tttctttaca gcacgaggag aagatgtaca agaaaaaaat agcacagctt tttataataa
     4201 tgcagaggtg tttgaagtgg tggaacgtgt agaagagtta agaaggaagt ggccagtagc
     4261 gtgggggaag ttagatgatg gcagtattgg tgtggtgact ccatatgctg atcaagtgtt
     4321 tagaatacgt gctgaacttc gaaaaaagag attatctgat gttaatgtag aaagggtgct
     4381 aaatgttcaa ggaaagcaat tcagagtttt gtttcttagc acagtacgta caagacatac
     4441 ttgtaaacat aaacagacac caattaaaaa gaaagagcaa cttctggaag attccacaga
     4501 ggacttagat tatggttttt tatctaacta caagcttctc aatactgcca tcacaagagc
     4561 acaatccctg gttgctgtgg tgggtgatcc cattgctctg tgctctattg gaagatgcag
     4621 gaaattttgg gaacggttta ttgccctgtg tcatgaaaac agtagcctac atggaatcac
     4681 ttttgaacag atcaaagccc agttagaggc tttagaacta aagaagacat atgtgttgaa
     4741 tccgctggca cctgaattta tcccccgggc tctaagactg cagcattcag gaagtaccaa
     4801 caaacagcag caatcaccac ccaaggggaa aagtcttcat catacccaga atgatcactt
     4861 ccagaatgat ggaattgttc agcccaatcc ttctgtactt attggcaatc ctattagagc
     4921 atatactcct ccaccccctc ttggacctca cccaaatttg ggaaaatctc caagccctgt
     4981 tcaaagaata gatcctcaca ctgggacaag tattctttat gtacctgctg tctatggagg
     5041 gaatgtagtt atgtcggtgc ctttacctgt accatggaca ggataccagg gtaggtttgc
     5101 agttgatcct cgaattatta cacatcaggc agcaatggcc tataacatga acctattaca
     5161 gacacatgga cgaggatctc ctattcctta tggccttgga catcacccac ctgtcaccat
     5221 aggccagcca caaaatcagc atcaggagaa ggatcaacat gagcaaaatc gaaatggtaa
     5281 aagtgataca aataattccg gacctgaaat taataagatt cgaacaccag agaaaaagcc
     5341 aacagaacca aaacaggttg atttggaatc aaatccacag aacagaagtc ctgaatcacg
     5401 tcctagtgtt gtttatccca gtaccaaatt tcctcgcaaa gataatctca acccaagaca
     5461 cataaatctt ccccttcctg ctccccacgc acagtatgca atccctaatc gccactttca
     5521 tccccttccc cagctaccaa gaccaccctt tccaattcca cagcagcaca ccttgttaaa
     5581 tcagcagcag aataatttgc ctgaacaacc aaatcagata ccacctcagc caaatcaggt
     5641 agtccagcag caaagtcagt tgaatcagca gcctcagcag ccacctcctc agctttctcc
     5701 tgcatatcag gcgggaccca acaatgcttt ttttaatagt gcagttgctc atcggccaca
     5761 gtctcctcct gcagaagctg taattccgga gcagcagccc cctcccatgc tgcaagaagg
     5821 ccacagtcct ctgagagcca ttgcacaacc cggccccatt taatctagat aactgatcat
     5881 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     5941 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     6001 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6061 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg
     6121 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     6181 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     6241 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     6301 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     6361 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     6421 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     6481 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     6541 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6601 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6661 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     6721 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6781 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     6841 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     6901 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     6961 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     7021 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     7081 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     7141 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     7201 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     7261 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     7321 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     7381 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     7441 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     7501 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     7561 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     7621 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     7681 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     7741 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     7801 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     7861 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     7921 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     7981 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     8041 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     8101 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     8161 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     8221 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     8281 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     8341 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     8401 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8461 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8521 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8581 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     8641 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8701 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8761 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8821 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8881 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8941 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9001 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9061 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9121 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9181 taccgccatg cat
//
