LOCUS       sequence-291366-       10795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGIGYF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10795)
  AUTHORS   Ruscica V, Bawankar P, Peter D, Helms S, Igreja C, Izaurralde E
  TITLE     Direct role for the Drosophila GIGYF protein in 4EHP-mediated mRNA 
            repression.
  JOURNAL   Nucleic Acids Res. 2019 Jul 26;47(13):7035-7048. doi: 
            10.1093/nar/gkz429.
  PUBMED    31114929
REFERENCE   2  (bases 1 to 10795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-07-26- 26"; volume: "47"; issue: 
            "13"; pages: "7035-7048"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmGIGYF_AH
FEATURES             Location/Qualifiers
     source          1..10795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8080..8121
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8131..8148
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8171..8188)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8221..8355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8245..8264)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8299..8318
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8977..9565)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9057..9076)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9736..10527,10528..10596))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10359..10378
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10597..10701)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10769..10787
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgaattcta tgacagattc aatgaaattt ggcccggaat ggttgcgcaa
     3361 tatgtcagcc gagccttcgg gctctcccag tacctacaac gttggtaatg cagctcaaaa
     3421 caattcgatt ggagggcaca acctgggaaa caacacggcg gcatccgctt cccgtaatct
     3481 atttccagaa taccggtacg gacgcgagga aatgctgtcc ttgttcgatc ggaactgcct
     3541 actgccgcag atcctgccat cgttcaaaaa gctcttcgtg gaaaaggttc agtgccctct
     3601 tgcactgaca ccgagctccg aggaggacat caaccaaaac tcacttggca ataactctcg
     3661 tcctgcctgg ttgcagcgct cgcccagtgg atttggtagt gcctcccgag gctctggacg
     3721 tggtggaacc gttgaccggg gacggatgcg cggaaaatcg gcctatcatc cgatatacca
     3781 acgcccaagc ggtctatatg atgaaagctt atcggttata tcaaaggccg aacgcacatg
     3841 gagcgaccgc aacggaactg gagattctgc ggcaaccacc acctccacta gtggctctgg
     3901 tgctctagat tggaatggaa ctccaagctc aagtcctcga aaagattatt ctaataatca
     3961 tcgcaacttg gaaaactggc gacgaacacg taacgaagat ggatccggtg atggtccatc
     4021 ctccagcggt tccatgagcg gacccgatat tgctggttgg cggagcggtg tcgtcggtgg
     4081 aagtacaaac gcaggttttg gtaccaatag ccatcggtgg ggaagatcta caagctggcg
     4141 tgatgaagat cctagcgtcg acaatgcagc tagtttacaa cgctcaattt caacagttgc
     4201 gactttaagt acagatcgga ctggaaacaa taaaggatct ggaatcggag ctgcagaggg
     4261 agtagggagc atatcgcacc cacggttgtc gagttctaaa atatctcaat tatggactgt
     4321 taataatgca gccggtgttg atgccgacga aaatctaccc gaatgggcaa ttgaaaatcc
     4381 atcgaaattg ggtggcagtt ttgatgctag tggagccttt catggagata ctgatctaaa
     4441 acctatcaaa agttcgcata atactttaaa aaccaaaagc ttagactcct atgacgatgt
     4501 aaaaagaccg aaaagtaaag acctgtctga tccagatagt ggaaacgata taactccaga
     4561 aacctcatta acgaaagata ccaatacaac agctgtgcaa gaagaagttg aaagcagttt
     4621 atctccaata agctctacaa cgacaaagga agtgatccat ggagatattt cagatcggat
     4681 taaagaggtc gccgatgaag tagaaaaact tataatggat gatgatcata aaatctcttc
     4741 gaatcaaagc caacatcaaa atcatgacgg gtttacagct gcacttccaa gaatggcaga
     4801 cattgagata aacgtaaaac cgagtgttac agcagtgcat cggcaagcac cttccaccat
     4861 gcctatacaa attaccccta caattacaga tgtcgctcca ccatcccatg cagttgtgtc
     4921 gttttcggat catgagacaa tgcaacacca taacatgcac cacctgccac aatttccaat
     4981 gattcctaca ccgcatatca tcactccaaa tctaaacgaa ttgtggtttt accgggatcc
     5041 gcaggcaaat gttcaggggc catttagtgc cgttgagatg acggaatggt atcgcgctgg
     5101 ctactttaat gagaacctgt ttgtacgccg gtactctgat aataggttta gaccgctggg
     5161 agagcttata aaattttgtc atggtaacat gccatttacg cacagtcacc tacttccatc
     5221 tccgatagaa ctagagaaca ttcctgttgg tcaaatacca gcccctctaa cggcgtcact
     5281 ttcaattaca ccccataagc catcaccgat tcctatcgca ctttctgttg ttgaacagca
     5341 gttgcagcaa caaagagatg agcaactaaa ggcaaatgtt acagcaacag ctgaagctct
     5401 acgtgctgca ataaaaggaa gttttggcgg aaatagcatt ggtaatacgt ctcatttgct
     5461 tacaatgcgg tttcaaatgc tacaggatca gtacatacag caccaggaat accaaatact
     5521 agctgagcta tccaaaaatg aatgctttca acgtctttcg gctgtcgagc aagaaacagt
     5581 tgttcgtcgg aaagttcaat tgctgggtct tcctgagtat ttgatcagct taaacggatt
     5641 aagcaattct ttgtccgtac tgaaccccgt tgccggtaga cagttgtaca gggcagtggt
     5701 tgagcacgcc aagaaggatc aacaacatat ttttgcaaac accgagcaac aacgttcagt
     5761 gggaaattta ctggatgcta ataatttcat tctaaatgcc caaataatga ttcagcaatc
     5821 gcagcaagag gtaggtccct tggtatcatc cgtggattgt attatgcaag gtggaactgc
     5881 agctgaccta aataagccga atgaaatacc gaggaatgag ttagacttaa ttaatgaata
     5941 taacttacgg atgctgttgc gtggccaacc aacaagtact caacagcaac cacctgcgct
     6001 gacgaactca gctaccgaaa atctccctgg agttgatttt ttaactgaaa cacaattgtt
     6061 agagcggcaa aacttgatga ttccaatctg gttaccccct aataataata agcaacaaga
     6121 aaccgaccaa cagtgggctg aaatgtctaa tgcggatgca tcattatggg aaacggccaa
     6181 cttaaatgaa gagcgaaatg aagatcagca actattaata caaaagtctt ctgaagcgtg
     6241 cttcgcagat acagaaaaag atgtcaaaat tgcccaatta tttcaagttc aatcaggaaa
     6301 tgttgttaac cacaccgcat tagaggaatt agatcagtcc cctcaaaatt taaaaggttc
     6361 acataatcaa aaaatagtca aatcccttgt ttccgatatt caacagaatc ataatgagga
     6421 actaaattca catcaacacc aggtaaagca ggctaacaag cagaatctga atacaaaaca
     6481 gaatgcggca cagtcagccc taaagccaat taataacgaa aatgatcgca aacgagaaca
     6541 gacagaagaa aaaaaaaggc aaagagagga acgcaagcgc cagcaattgg aagatgataa
     6601 acgtcgggcg ttgaatgaat ctgaagaaca gacccgccaa attcaagagg aaaaggaaag
     6661 gcaacagcaa atacaagccc aacgtcgaaa ggcattgttg ggcaatgtcc attctctatc
     6721 agttcaaaat gggatgtcag gaacattagc gtctgcacaa agcaagaaga acgacgatgc
     6781 caaaacagca gaaccccaag tatcctcgcg cttgccgtct acatccgtag cgccttggtc
     6841 ttttcagttg caaaattcta tgagatcggc gcctggtctg gcagaaatac aaaaggcaga
     6901 acgtcgagag cgtcgcgcag accaacagcg acatcaagag ctattagaca agcaattgcg
     6961 tgccaatgcc gcagctgcgg ctgaagccaa cgatgctttg cttaaatggc agtcaacacc
     7021 agcgtcggcg cccgtaatga gtctcgccga aattcaagca gaagaggcaa ggcggttggc
     7081 caatgacctt gtggatcggc agcgtcgacg agaactggaa catcaccaac aagctccttt
     7141 gtcatcagcg gttttggtta cgagtgcaac ttccaacatc tggggtaacg ccaataaagc
     7201 atggagttcg tccgctgctc aatcactttc gttaaaaaca agttctggaa ctggtctgtg
     7261 ggacgaaccg aatccattag gttctaatgg atctggaaca agcggtacct catccgtaac
     7321 tgcggcggca gtcttggcag gaggattaaa ctcagctaat aaatccactc tgcaagctca
     7381 aaataagtcg tcggctttat ttgcatcgcc tcgaaatttg cgcaagagtc aaacattgcc
     7441 agcccttagt aacccagaaa agtcaaataa aaatggacca ggtcaacggc cagaaaaaca
     7501 aaagttggct caaactcgtt caaagggtgc cgctgtctca attgaagaga aggatagaga
     7561 aagaaaattg aatgcaaaaa gccagcaaag cagcaccgac caagccatta gcaaggttaa
     7621 tgagtatgaa aacgagttca ctagctggtg cataaagagc ctagataata tgtccgccaa
     7681 agttgatgta cccacgttcg ttgcattctt gcaggacttg gaagcgccat atgaggtaaa
     7741 ggactatgtc cgaatatacc ttggcgatgg aaaagattct ttggatttcg caaagcagtt
     7801 tttggagcga cgtagcaaat acaaaagctt gcaacgtgcc caaaaggcac acaatgacga
     7861 tatgtgcaaa ccagctcctg ccattacgcc atctgcgaat gactatgccg acagcaagaa
     7921 caaacaaaaa aagattaaaa agaataagat gactaagatg gacgcccgca ttcttgggtt
     7981 ttcagtaact gctgccgagg gtcgcataaa tgttggcatt cgagactacg tcgaaggacc
     8041 ataagcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat ccctaaccct
     8101 ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc
     8161 cgctgatcag cctcgactgt gccttctaag gcctgagctc gctgatcagc ctcgatcgag
     8221 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     8281 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     8341 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     8401 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     8461 cagtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     8521 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     8581 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
     8641 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
     8701 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc
     8761 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
     8821 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
     8881 agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca gccagcaaaa
     8941 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9001 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9061 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9121 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     9181 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9241 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9301 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9361 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     9421 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9481 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9541 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9601 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9661 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9721 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9781 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9841 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9901 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9961 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10021 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
    10081 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10141 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10201 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10261 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10321 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
    10381 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10441 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10501 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10561 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10621 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10681 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    10741 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgt
//
