LOCUS       sequence-291376-       10117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGIGYF1-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10117)
  AUTHORS   Ruscica V, Bawankar P, Peter D, Helms S, Igreja C, Izaurralde E
  TITLE     Direct role for the Drosophila GIGYF protein in 4EHP-mediated mRNA 
            repression.
  JOURNAL   Nucleic Acids Res. 2019 Jul 26;47(13):7035-7048. doi: 
            10.1093/nar/gkz429.
  PUBMED    31114929
REFERENCE   2  (bases 1 to 10117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-07-26- 26"; volume: "47"; issue: 
            "13"; pages: "7035-7048"
COMMENT     Sequence Label: pAc5.1B-HA-DmGIGYF-dsRNAres_AH
FEATURES             Location/Qualifiers
     source          1..10117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2594..2620
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7402..7443
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7453..7470
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7493..7510)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7543..7677
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7567..7586)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7621..7640
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8299..8887)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8379..8398)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9058..9849,9850..9918))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9681..9700
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9919..10023)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10091..10109
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gcttacccat acgatgttcc agattacgct cctcctccaa gcttgaattc
     2641 tatgactgac tccatgaagt tcggacctga gtggctgcga aacatgagcg cagaaccctc
     2701 cggtagccca tccacgtata atgtgggaaa cgcggcccag aataacagca taggcggaca
     2761 taatctcggc aataatactg cagcgtcggc atcgcgaaac ctcttccccg agtatcgcta
     2821 tggtcgggaa gagatgctca gtctctttga ccgtaattgt ctgctcccac aaattttgcc
     2881 ttcctttaag aaactgtttg tcgagaaagt ccaatgtccg ctggcgctca cccctagtag
     2941 cgaagaagat attaatcaga attccctcgg aaacaatagc cgcccagcat ggctccaaag
     3001 gtccccgtcg ggtttcggat ccgctagtcg cggaagcggt cgtggcggta cagtggatcg
     3061 tggtcgcatg aggggcaagt ccgcgtacca cccaatttat cagcgtcctt cgggcctgta
     3121 cgacgagagt ctctccgtga tcagtaaagc tgagcggact tggtccgatc gtaatggcac
     3181 aggcgacagt gctgctacaa ctactagtac gtcgggtagt ggagcgttgg actggaacgg
     3241 tacgcctagt agctcgccac ggaaggacta ctccaacaac caccgtaatc tggagaattg
     3301 gcgtcggact cgaaatgagg acggcagtgg cgacggcccg tcgagttccg gctcgatgag
     3361 tggccctgat attgctggtt ggcggagcgg tgtcgtcggt ggaagtacaa acgcaggttt
     3421 tggtaccaat agccatcggt ggggaagatc tacaagctgg cgtgatgaag atcctagcgt
     3481 cgacaatgca gctagtttac aacgctcaat ttcaacagtt gcgactttaa gtacagatcg
     3541 gactggaaac aataaaggat ctggaatcgg agctgcagag ggagtaggga gcatatcgca
     3601 cccacggttg tcgagttcta aaatatctca attatggact gttaataatg cagccggtgt
     3661 tgatgccgac gaaaatctac ccgaatgggc aattgaaaat ccatcgaaat tgggtggcag
     3721 ttttgatgct agtggagcct ttcatggaga tactgatcta aaacctatca aaagttcgca
     3781 taatacttta aaaaccaaaa gcttagactc ctatgacgat gtaaaaagac cgaaaagtaa
     3841 agacctgtct gatccagata gtggaaacga tataactcca gaaacctcat taacgaaaga
     3901 taccaataca acagctgtgc aagaagaagt tgaaagcagt ttatctccaa taagctctac
     3961 aacgacaaag gaagtgatcc atggagatat ttcagatcgg attaaagagg tcgccgatga
     4021 agtagaaaaa cttataatgg atgatgatca taaaatctct tcgaatcaaa gccaacatca
     4081 aaatcatgac gggtttacag ctgcacttcc aagaatggca gacattgaga taaacgtaaa
     4141 accgagtgtt acagcagtgc atcggcaagc accttccacc atgcctatac aaattacccc
     4201 tacaattaca gatgtcgctc caccatccca tgcagttgtg tcgttttcgg atcatgagac
     4261 aatgcaacac cataacatgc accacctgcc acaatttcca atgattccta caccgcatat
     4321 catcactcca aatctaaacg aattgtggtt ttaccgggat ccgcaggcaa atgttcaggg
     4381 gccatttagt gccgttgaga tgacggaatg gtatcgcgct ggctacttta atgagaacct
     4441 gtttgtacgc cggtactctg ataataggtt tagaccgctg ggagagctta taaaattttg
     4501 tcatggtaac atgccattta cgcacagtca cctacttcca tctccgatag aactagagaa
     4561 cattcctgtt ggtcaaatac cagcccctct aacggcgtca ctttcaatta caccccataa
     4621 gccatcaccg attcctatcg cactttctgt tgttgaacag cagttgcagc aacaaagaga
     4681 tgagcaacta aaggcaaatg ttacagcaac agctgaagct ctacgtgctg caataaaagg
     4741 aagttttggc ggaaatagca ttggtaatac gtctcatttg cttacaatgc ggtttcaaat
     4801 gctacaggat cagtacatac agcaccagga ataccaaata ctagctgagc tatccaaaaa
     4861 tgaatgcttt caacgtcttt cggctgtcga gcaagaaaca gttgttcgtc ggaaagttca
     4921 attgctgggt cttcctgagt atttgatcag cttaaacgga ttaagcaatt ctttgtccgt
     4981 actgaacccc gttgccggta gacagttgta cagggcagtg gttgagcacg ccaagaagga
     5041 tcaacaacat atttttgcaa acaccgagca acaacgttca gtgggaaatt tactggatgc
     5101 taataatttc attctaaatg cccaaataat gattcagcaa tcgcagcaag aggtaggtcc
     5161 cttggtatca tccgtggatt gtattatgca aggtggaact gcagctgacc taaataagcc
     5221 gaatgaaata ccgaggaatg agttagactt aattaatgaa tataacttac ggatgctgtt
     5281 gcgtggccaa ccaacaagta ctcaacagca accacctgcg ctgacgaact cagctaccga
     5341 aaatctccct ggagttgatt ttttaactga aacacaattg ttagagcggc aaaacttgat
     5401 gattccaatc tggttacccc ctaataataa taagcaacaa gaaaccgacc aacagtgggc
     5461 tgaaatgtct aatgcggatg catcattatg ggaaacggcc aacttaaatg aagagcgaaa
     5521 tgaagatcag caactattaa tacaaaagtc ttctgaagcg tgcttcgcag atacagaaaa
     5581 agatgtcaaa attgcccaat tatttcaagt tcaatcagga aatgttgtta accacaccgc
     5641 attagaggaa ttagatcagt cccctcaaaa tttaaaaggt tcacataatc aaaaaatagt
     5701 caaatccctt gtttccgata ttcaacagaa tcataatgag gaactaaatt cacatcaaca
     5761 ccaggtaaag caggctaaca agcagaatct gaatacaaaa cagaatgcgg cacagtcagc
     5821 cctaaagcca attaataacg aaaatgatcg caaacgagaa cagacagaag aaaaaaaaag
     5881 gcaaagagag gaacgcaagc gccagcaatt ggaagatgat aaacgtcggg cgttgaatga
     5941 atctgaagaa cagacccgcc aaattcaaga ggaaaaggaa aggcaacagc aaatacaagc
     6001 ccaacgtcga aaggcattgt tgggcaatgt ccattctcta tcagttcaaa atgggatgtc
     6061 aggaacatta gcgtctgcac aaagcaagaa gaacgacgat gccaaaacag cagaacccca
     6121 agtatcctcg cgcttgccgt ctacatccgt agcgccttgg tcttttcagt tgcaaaattc
     6181 tatgagatcg gcgcctggtc tggcagaaat acaaaaggca gaacgtcgag agcgtcgcgc
     6241 agaccaacag cgacatcaag agctattaga caagcaattg cgtgccaatg ccgcagctgc
     6301 ggctgaagcc aacgatgctt tgcttaaatg gcagtcaaca ccagcgtcgg cgcccgtaat
     6361 gagtctcgcc gaaattcaag cagaagaggc aaggcggttg gccaatgacc ttgtggatcg
     6421 gcagcgtcga cgagaactgg aacatcacca acaagctcct ttgtcatcag cggttttggt
     6481 tacgagtgca acttccaaca tctggggtaa cgccaataaa gcatggagtt cgtccgctgc
     6541 tcaatcactt tcgttaaaaa caagttctgg aactggtctg tgggacgaac cgaatccatt
     6601 aggttctaat ggatctggaa caagcggtac ctcatccgta actgcggcgg cagtcttggc
     6661 aggaggatta aactcagcta ataaatccac tctgcaagct caaaataagt cgtcggcttt
     6721 atttgcatcg cctcgaaatt tgcgcaagag tcaaacattg ccagccctta gtaacccaga
     6781 aaagtcaaat aaaaatggac caggtcaacg gccagaaaaa caaaagttgg ctcaaactcg
     6841 ttcaaagggt gccgctgtct caattgaaga gaaggataga gaaagaaaat tgaatgcaaa
     6901 aagccagcaa agcagcaccg accaagccat tagcaaggtt aatgagtatg aaaacgagtt
     6961 cactagctgg tgcataaaga gcctagataa tatgtccgcc aaagttgatg tacccacgtt
     7021 cgttgcattc ttgcaggact tggaagcgcc atatgaggta aaggactatg tccgaatata
     7081 ccttggcgat ggaaaagatt ctttggattt cgcaaagcag tttttggagc gacgtagcaa
     7141 atacaaaagc ttgcaacgtg cccaaaaggc acacaatgac gatatgtgca aaccagctcc
     7201 tgccattacg ccatctgcga atgactatgc cgacagcaag aacaaacaaa aaaagattaa
     7261 aaagaataag atgactaaga tggacgcccg cattcttggg ttttcagtaa ctgctgccga
     7321 gggtcgcata aatgttggca ttcgagacta cgtcgaagga ccataagcgg ccgctcgagt
     7381 ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
     7441 acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact
     7501 gtgccttcta aggcctgagc tcgctgatca gcctcgatcg aggatccaga catgataaga
     7561 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     7621 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     7681 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     7741 agcaagtaaa acctctacaa atgtggtatg gctgattatg atcagtcgac cgatgccctt
     7801 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
     7861 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt
     7921 cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt
     7981 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt
     8041 cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct
     8101 ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg
     8161 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg
     8221 acagcttcaa ggatcgctcg cggctcttac cagccagcaa aaggccagga accgtaaaaa
     8281 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8341 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8401 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8461 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     8521 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8581 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8641 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8701 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     8761 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8821 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8881 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8941 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9001 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9061 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9121 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9181 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9241 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9301 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9361 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9421 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9481 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9541 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9601 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9661 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9721 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9781 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9841 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9901 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9961 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10021 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    10081 aacctataaa aataggcgta tcacgaggcc ctttcgt
//
