LOCUS       sequence-291380-       10198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGIGYF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   Ruscica V, Bawankar P, Peter D, Helms S, Igreja C, Izaurralde E
  TITLE     Direct role for the Drosophila GIGYF protein in 4EHP-mediated mRNA 
            repression.
  JOURNAL   Nucleic Acids Res. 2019 Jul 26;47(13):7035-7048. doi: 
            10.1093/nar/gkz429.
  PUBMED    31114929
REFERENCE   2  (bases 1 to 10198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-07-26- 26"; volume: "47"; issue: 
            "13"; pages: "7035-7048"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGIGYF_AH
FEATURES             Location/Qualifiers
     source          1..10198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7483..7524
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7534..7551
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7574..7591)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7624..7758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7648..7667)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7702..7721
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8380..8968)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8460..8479)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9139..9930,9931..9999))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9762..9781
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10000..10104)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10172..10190
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctatgacaga ttcaatgaaa tttggcccgg aatggttgcg
     2761 caatatgtca gccgagcctt cgggctctcc cagtacctac aacgttggta atgcagctca
     2821 aaacaattcg attggagggc acaacctggg aaacaacacg gcggcatccg cttcccgtaa
     2881 tctatttcca gaataccggt acggacgcga ggaaatgctg tccttgttcg atcggaactg
     2941 cctactgccg cagatcctgc catcgttcaa aaagctcttc gtggaaaagg ttcagtgccc
     3001 tcttgcactg acaccgagct ccgaggagga catcaaccaa aactcacttg gcaataactc
     3061 tcgtcctgcc tggttgcagc gctcgcccag tggatttggt agtgcctccc gaggctctgg
     3121 acgtggtgga accgttgacc ggggacggat gcgcggaaaa tcggcctatc atccgatata
     3181 ccaacgccca agcggtctat atgatgaaag cttatcggtt atatcaaagg ccgaacgcac
     3241 atggagcgac cgcaacggaa ctggagattc tgcggcaacc accacctcca ctagtggctc
     3301 tggtgctcta gattggaatg gaactccaag ctcaagtcct cgaaaagatt attctaataa
     3361 tcatcgcaac ttggaaaact ggcgacgaac acgtaacgaa gatggatccg gtgatggtcc
     3421 atcctccagc ggttccatga gcggacccga tattgctggt tggcggagcg gtgtcgtcgg
     3481 tggaagtaca aacgcaggtt ttggtaccaa tagccatcgg tggggaagat ctacaagctg
     3541 gcgtgatgaa gatcctagcg tcgacaatgc agctagttta caacgctcaa tttcaacagt
     3601 tgcgacttta agtacagatc ggactggaaa caataaagga tctggaatcg gagctgcaga
     3661 gggagtaggg agcatatcgc acccacggtt gtcgagttct aaaatatctc aattatggac
     3721 tgttaataat gcagccggtg ttgatgccga cgaaaatcta cccgaatggg caattgaaaa
     3781 tccatcgaaa ttgggtggca gttttgatgc tagtggagcc tttcatggag atactgatct
     3841 aaaacctatc aaaagttcgc ataatacttt aaaaaccaaa agcttagact cctatgacga
     3901 tgtaaaaaga ccgaaaagta aagacctgtc tgatccagat agtggaaacg atataactcc
     3961 agaaacctca ttaacgaaag ataccaatac aacagctgtg caagaagaag ttgaaagcag
     4021 tttatctcca ataagctcta caacgacaaa ggaagtgatc catggagata tttcagatcg
     4081 gattaaagag gtcgccgatg aagtagaaaa acttataatg gatgatgatc ataaaatctc
     4141 ttcgaatcaa agccaacatc aaaatcatga cgggtttaca gctgcacttc caagaatggc
     4201 agacattgag ataaacgtaa aaccgagtgt tacagcagtg catcggcaag caccttccac
     4261 catgcctata caaattaccc ctacaattac agatgtcgct ccaccatccc atgcagttgt
     4321 gtcgttttcg gatcatgaga caatgcaaca ccataacatg caccacctgc cacaatttcc
     4381 aatgattcct acaccgcata tcatcactcc aaatctaaac gaattgtggt tttaccggga
     4441 tccgcaggca aatgttcagg ggccatttag tgccgttgag atgacggaat ggtatcgcgc
     4501 tggctacttt aatgagaacc tgtttgtacg ccggtactct gataataggt ttagaccgct
     4561 gggagagctt ataaaatttt gtcatggtaa catgccattt acgcacagtc acctacttcc
     4621 atctccgata gaactagaga acattcctgt tggtcaaata ccagcccctc taacggcgtc
     4681 actttcaatt acaccccata agccatcacc gattcctatc gcactttctg ttgttgaaca
     4741 gcagttgcag caacaaagag atgagcaact aaaggcaaat gttacagcaa cagctgaagc
     4801 tctacgtgct gcaataaaag gaagttttgg cggaaatagc attggtaata cgtctcattt
     4861 gcttacaatg cggtttcaaa tgctacagga tcagtacata cagcaccagg aataccaaat
     4921 actagctgag ctatccaaaa atgaatgctt tcaacgtctt tcggctgtcg agcaagaaac
     4981 agttgttcgt cggaaagttc aattgctggg tcttcctgag tatttgatca gcttaaacgg
     5041 attaagcaat tctttgtccg tactgaaccc cgttgccggt agacagttgt acagggcagt
     5101 ggttgagcac gccaagaagg atcaacaaca tatttttgca aacaccgagc aacaacgttc
     5161 agtgggaaat ttactggatg ctaataattt cattctaaat gcccaaataa tgattcagca
     5221 atcgcagcaa gaggtaggtc ccttggtatc atccgtggat tgtattatgc aaggtggaac
     5281 tgcagctgac ctaaataagc cgaatgaaat accgaggaat gagttagact taattaatga
     5341 atataactta cggatgctgt tgcgtggcca accaacaagt actcaacagc aaccacctgc
     5401 gctgacgaac tcagctaccg aaaatctccc tggagttgat tttttaactg aaacacaatt
     5461 gttagagcgg caaaacttga tgattccaat ctggttaccc cctaataata ataagcaaca
     5521 agaaaccgac caacagtggg ctgaaatgtc taatgcggat gcatcattat gggaaacggc
     5581 caacttaaat gaagagcgaa atgaagatca gcaactatta atacaaaagt cttctgaagc
     5641 gtgcttcgca gatacagaaa aagatgtcaa aattgcccaa ttatttcaag ttcaatcagg
     5701 aaatgttgtt aaccacaccg cattagagga attagatcag tcccctcaaa atttaaaagg
     5761 ttcacataat caaaaaatag tcaaatccct tgtttccgat attcaacaga atcataatga
     5821 ggaactaaat tcacatcaac accaggtaaa gcaggctaac aagcagaatc tgaatacaaa
     5881 acagaatgcg gcacagtcag ccctaaagcc aattaataac gaaaatgatc gcaaacgaga
     5941 acagacagaa gaaaaaaaaa ggcaaagaga ggaacgcaag cgccagcaat tggaagatga
     6001 taaacgtcgg gcgttgaatg aatctgaaga acagacccgc caaattcaag aggaaaagga
     6061 aaggcaacag caaatacaag cccaacgtcg aaaggcattg ttgggcaatg tccattctct
     6121 atcagttcaa aatgggatgt caggaacatt agcgtctgca caaagcaaga agaacgacga
     6181 tgccaaaaca gcagaacccc aagtatcctc gcgcttgccg tctacatccg tagcgccttg
     6241 gtcttttcag ttgcaaaatt ctatgagatc ggcgcctggt ctggcagaaa tacaaaaggc
     6301 agaacgtcga gagcgtcgcg cagaccaaca gcgacatcaa gagctattag acaagcaatt
     6361 gcgtgccaat gccgcagctg cggctgaagc caacgatgct ttgcttaaat ggcagtcaac
     6421 accagcgtcg gcgcccgtaa tgagtctcgc cgaaattcaa gcagaagagg caaggcggtt
     6481 ggccaatgac cttgtggatc ggcagcgtcg acgagaactg gaacatcacc aacaagctcc
     6541 tttgtcatca gcggttttgg ttacgagtgc aacttccaac atctggggta acgccaataa
     6601 agcatggagt tcgtccgctg ctcaatcact ttcgttaaaa acaagttctg gaactggtct
     6661 gtgggacgaa ccgaatccat taggttctaa tggatctgga acaagcggta cctcatccgt
     6721 aactgcggcg gcagtcttgg caggaggatt aaactcagct aataaatcca ctctgcaagc
     6781 tcaaaataag tcgtcggctt tatttgcatc gcctcgaaat ttgcgcaaga gtcaaacatt
     6841 gccagccctt agtaacccag aaaagtcaaa taaaaatgga ccaggtcaac ggccagaaaa
     6901 acaaaagttg gctcaaactc gttcaaaggg tgccgctgtc tcaattgaag agaaggatag
     6961 agaaagaaaa ttgaatgcaa aaagccagca aagcagcacc gaccaagcca ttagcaaggt
     7021 taatgagtat gaaaacgagt tcactagctg gtgcataaag agcctagata atatgtccgc
     7081 caaagttgat gtacccacgt tcgttgcatt cttgcaggac ttggaagcgc catatgaggt
     7141 aaaggactat gtccgaatat accttggcga tggaaaagat tctttggatt tcgcaaagca
     7201 gtttttggag cgacgtagca aatacaaaag cttgcaacgt gcccaaaagg cacacaatga
     7261 cgatatgtgc aaaccagctc ctgccattac gccatctgcg aatgactatg ccgacagcaa
     7321 gaacaaacaa aaaaagatta aaaagaataa gatgactaag atggacgccc gcattcttgg
     7381 gttttcagta actgctgccg agggtcgcat aaatgttggc attcgagact acgtcgaagg
     7441 accataagcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac
     7501 cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa
     7561 acccgctgat cagcctcgac tgtgccttct aaggcctgag ctcgctgatc agcctcgatc
     7621 gaggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     7681 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     7741 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     7801 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     7861 gatcagtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
     7921 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
     7981 ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat
     8041 gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac
     8101 tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga
     8161 cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat
     8221 gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca
     8281 ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta ccagccagca
     8341 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8401 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8461 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8521 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     8581 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8641 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8701 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8761 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8821 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8881 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8941 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9001 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9061 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9121 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9181 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9241 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9301 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9361 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9421 agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc
     9481 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9541 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9601 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9661 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9721 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     9781 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9841 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9901 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9961 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10021 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10081 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    10141 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgt
//
