LOCUS       sequence-291384-        8278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGIGYF1_641-1574.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Ruscica V, Bawankar P, Peter D, Helms S, Igreja C, Izaurralde E
  TITLE     Direct role for the Drosophila GIGYF protein in 4EHP-mediated mRNA 
            repression.
  JOURNAL   Nucleic Acids Res. 2019 Jul 26;47(13):7035-7048. doi: 
            10.1093/nar/gkz429.
  PUBMED    31114929
REFERENCE   2  (bases 1 to 8278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-07-26- 26"; volume: "47"; issue: 
            "13"; pages: "7035-7048"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGIGYF_641-1574_AH
FEATURES             Location/Qualifiers
     source          1..8278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5563..5604
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5614..5631
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5654..5671)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5704..5838
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5728..5747)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5782..5801
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6460..7048)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6540..6559)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7219..8010,8011..8079))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7842..7861
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8080..8184)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8252..8270
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctattcctgt tggtcaaata ccagcccctc taacggcgtc
     2761 actttcaatt acaccccata agccatcacc gattcctatc gcactttctg ttgttgaaca
     2821 gcagttgcag caacaaagag atgagcaact aaaggcaaat gttacagcaa cagctgaagc
     2881 tctacgtgct gcaataaaag gaagttttgg cggaaatagc attggtaata cgtctcattt
     2941 gcttacaatg cggtttcaaa tgctacagga tcagtacata cagcaccagg aataccaaat
     3001 actagctgag ctatccaaaa atgaatgctt tcaacgtctt tcggctgtcg agcaagaaac
     3061 agttgttcgt cggaaagttc aattgctggg tcttcctgag tatttgatca gcttaaacgg
     3121 attaagcaat tctttgtccg tactgaaccc cgttgccggt agacagttgt acagggcagt
     3181 ggttgagcac gccaagaagg atcaacaaca tatttttgca aacaccgagc aacaacgttc
     3241 agtgggaaat ttactggatg ctaataattt cattctaaat gcccaaataa tgattcagca
     3301 atcgcagcaa gaggtaggtc ccttggtatc atccgtggat tgtattatgc aaggtggaac
     3361 tgcagctgac ctaaataagc cgaatgaaat accgaggaat gagttagact taattaatga
     3421 atataactta cggatgctgt tgcgtggcca accaacaagt actcaacagc aaccacctgc
     3481 gctgacgaac tcagctaccg aaaatctccc tggagttgat tttttaactg aaacacaatt
     3541 gttagagcgg caaaacttga tgattccaat ctggttaccc cctaataata ataagcaaca
     3601 agaaaccgac caacagtggg ctgaaatgtc taatgcggat gcatcattat gggaaacggc
     3661 caacttaaat gaagagcgaa atgaagatca gcaactatta atacaaaagt cttctgaagc
     3721 gtgcttcgca gatacagaaa aagatgtcaa aattgcccaa ttatttcaag ttcaatcagg
     3781 aaatgttgtt aaccacaccg cattagagga attagatcag tcccctcaaa atttaaaagg
     3841 ttcacataat caaaaaatag tcaaatccct tgtttccgat attcaacaga atcataatga
     3901 ggaactaaat tcacatcaac accaggtaaa gcaggctaac aagcagaatc tgaatacaaa
     3961 acagaatgcg gcacagtcag ccctaaagcc aattaataac gaaaatgatc gcaaacgaga
     4021 acagacagaa gaaaaaaaaa ggcaaagaga ggaacgcaag cgccagcaat tggaagatga
     4081 taaacgtcgg gcgttgaatg aatctgaaga acagacccgc caaattcaag aggaaaagga
     4141 aaggcaacag caaatacaag cccaacgtcg aaaggcattg ttgggcaatg tccattctct
     4201 atcagttcaa aatgggatgt caggaacatt agcgtctgca caaagcaaga agaacgacga
     4261 tgccaaaaca gcagaacccc aagtatcctc gcgcttgccg tctacatccg tagcgccttg
     4321 gtcttttcag ttgcaaaatt ctatgagatc ggcgcctggt ctggcagaaa tacaaaaggc
     4381 agaacgtcga gagcgtcgcg cagaccaaca gcgacatcaa gagctattag acaagcaatt
     4441 gcgtgccaat gccgcagctg cggctgaagc caacgatgct ttgcttaaat ggcagtcaac
     4501 accagcgtcg gcgcccgtaa tgagtctcgc cgaaattcaa gcagaagagg caaggcggtt
     4561 ggccaatgac cttgtggatc ggcagcgtcg acgagaactg gaacatcacc aacaagctcc
     4621 tttgtcatca gcggttttgg ttacgagtgc aacttccaac atctggggta acgccaataa
     4681 agcatggagt tcgtccgctg ctcaatcact ttcgttaaaa acaagttctg gaactggtct
     4741 gtgggacgaa ccgaatccat taggttctaa tggatctgga acaagcggta cctcatccgt
     4801 aactgcggcg gcagtcttgg caggaggatt aaactcagct aataaatcca ctctgcaagc
     4861 tcaaaataag tcgtcggctt tatttgcatc gcctcgaaat ttgcgcaaga gtcaaacatt
     4921 gccagccctt agtaacccag aaaagtcaaa taaaaatgga ccaggtcaac ggccagaaaa
     4981 acaaaagttg gctcaaactc gttcaaaggg tgccgctgtc tcaattgaag agaaggatag
     5041 agaaagaaaa ttgaatgcaa aaagccagca aagcagcacc gaccaagcca ttagcaaggt
     5101 taatgagtat gaaaacgagt tcactagctg gtgcataaag agcctagata atatgtccgc
     5161 caaagttgat gtacccacgt tcgttgcatt cttgcaggac ttggaagcgc catatgaggt
     5221 aaaggactat gtccgaatat accttggcga tggaaaagat tctttggatt tcgcaaagca
     5281 gtttttggag cgacgtagca aatacaaaag cttgcaacgt gcccaaaagg cacacaatga
     5341 cgatatgtgc aaaccagctc ctgccattac gccatctgcg aatgactatg ccgacagcaa
     5401 gaacaaacaa aaaaagatta aaaagaataa gatgactaag atggacgccc gcattcttgg
     5461 gttttcagta actgctgccg agggtcgcat aaatgttggc attcgagact acgtcgaagg
     5521 accataagcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac
     5581 cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa
     5641 acccgctgat cagcctcgac tgtgccttct aaggcctgag ctcgctgatc agcctcgatc
     5701 gaggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     5761 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     5821 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     5881 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     5941 gatcagtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
     6001 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
     6061 ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat
     6121 gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac
     6181 tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga
     6241 cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat
     6301 gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca
     6361 ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta ccagccagca
     6421 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6481 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6541 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6601 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     6661 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6721 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6781 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6841 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6901 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6961 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7021 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7081 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7141 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7201 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7261 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7321 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7381 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7441 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7501 agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc
     7561 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7621 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7681 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7741 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7801 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     7861 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7921 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7981 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8041 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8101 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8161 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     8221 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgt
//
