LOCUS       sequence-291417-        8480 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Env gene from HIV patient plasma in pcDNA3.1 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8480)
  AUTHORS   Joshi A, Sedano M, Beauchamp B, Punke EB, Mulla ZD, Meza A, Alozie 
            OK, Mukherjee D, Garg H
  TITLE     HIV-1 Env Glycoprotein Phenotype along with Immune Activation 
            Determines CD4 T Cell Loss in HIV Patients.
  JOURNAL   J Immunol. 2016 Feb 15;196(4):1768-79. doi: 
            10.4049/jimmunol.1501588. Epub 2016 Jan 13.
  PUBMED    26764036
REFERENCE   2  (bases 1 to 8480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1501588"; journalName: "J Immunol"; date: 
            "2016-02-15- 15"; volume: "196"; issue: "4"; pages: "1768-79"
COMMENT     Sequence Label: pHP003Env
FEATURES             Location/Qualifiers
     source          1..8480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        156..359
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     310..330
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     404..423
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        404..422
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3519..3560
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3570..3587
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3610..3627)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3616..3840
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3886..4314
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3973..3992)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4183..4204
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4323..4343)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4328..4657
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4508..4643
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4570..4589
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4724..5518
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4778..4797)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5388..5407
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5694..5815
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5731..5750)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5785..5804
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5864..5880)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5864..5880)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5877..5899)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5888..5904
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5912..5918,5919..5936,5937..5942))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5957..5978
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6095..6112)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6266..6854)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6346..6365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7025..7816,7817..7885))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7648..7667
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7886..7990)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8065..8084)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8256..8480,1..155)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
       61 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      121 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      181 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      241 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      301 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
      361 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      421 gggagaccca agctggctag ttaagcttgg taccgagctc ggatccagta cccttcaccg
      481 atcaagcttt aggcatctcc tatggcagga agaagcggag acagcgacga agacctcctc
      541 aaggcagtca gagtcatcaa gttcctctat caaatcagta agtagtatat gtcatgtata
      601 tagcaataac agtagcagca tttgtagtag taacaataat agcaatagtt gtgtggtcca
      661 tagtaatcct agaatatagg aaaatattaa aacaaagaaa gatagacagg ttaattgata
      721 ggatagcaga aagagcagaa gacagtggca atgagagtga aggggatcag gaagaattat
      781 cagcacttgt ggagatgggg cgtaatgctc cttgggatgt taatgatcgg taaagctaca
      841 gaagattggt gggtcacagt ctattatggg gtacctgtgt ggagggatgc gaacaccact
      901 ctattttgtg catcaaatgc taaagcacat gttacagaag tacataatat ttgggccacg
      961 catgcctgtg tacccacaga ccccagccca caagaagtag tattggaaaa tgtgacagaa
     1021 aattttaaca tgtggaaaaa taatatggta gaacagatgc atgaggatgt aatcagttta
     1081 tgggatcaaa gcctaaaacc atgtgcaaaa ttaactccac tctgtgttac tctaaattgc
     1141 actgatttaa ggaataaaac agtcaatgct actgatacta aaggagagat gaaaaactgc
     1201 tctttcaatg tcaccacatt catgcaagat aaggtaaaga aagaatatgc acttttttat
     1261 agactagatg tagtaccaat aaatgaagct aataagacta acaataccag ctataccagc
     1321 tataggttga taaattgtaa tacctcagcc atcacacagg cctgtccaaa ggtaaccttt
     1381 gatccaattc ccatacatta ttgtgccccg gctggttttg cgattataaa gtgtaataat
     1441 aagacattca atggaacagg accatgtaca aatgtcagca cagtacaatg tacacatgga
     1501 attaaaccag tggtgtcaac tcaactgttg ttaaatggca gtctagcaga agaaggggta
     1561 atgcttagat caaaaaattt ctcagacaat gctaaaaaca taatagtaca gctgaaagag
     1621 cctgtaacaa ttaattgtac aagacccaac aacaatacaa gaaaaagtat aaatatggga
     1681 ccagggagag cattttgggc aacaggggat ataataggag atataagaca ggcacactgt
     1741 aacattagta gagaacaatg gaacaacact ttaaaacaga tagttgcaaa attaaaagaa
     1801 caatttaata ataaaacaat agtctttaat cactcctcag gaggggatcc agaaattgta
     1861 atgcaccatt ttaattgtca aggagaattt ttctactgtg atacaacaca attgtttaat
     1921 agtacttgga atgggactaa tactgagaat aaaactaaag agtcagatga cactactatc
     1981 acactcccat gtaggataaa acaaattata aacatgtggc aggaagtagg aagagcaatg
     2041 tatgcccctc ccatcagcgg gcaaattaac tgttcatcaa atattacagg gctgatatta
     2101 ataagagatg gtggagacaa gaatactacc gagaccttca gacctggagg aggagatatg
     2161 agggacaatt ggagaagtga attatataaa tacaaagtag tgagccttga gccaatagga
     2221 atagcaccaa caaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgaccctg
     2281 ggagctatgt tccttggatt cttgggagca gcaggaagca ctatgggcgc agcatcaata
     2341 acgctgacgg tacaggccag acaattattg tctggtatag tgcaacagca gaataatctg
     2401 ctgagagcta ttgaggcgca acagcatatg ttgcaactca cagtctgggg catcaaacag
     2461 ctccaggcaa gagtcctggc tgtggaaaga tacctaaggg atcaacggct cctgggaatt
     2521 tggggttgct ctggaaaact catctgcacc actactgtgc cttggaatac tagttggggg
     2581 ggtagtaata gaactctgaa tcagatatgg gataacatga cctggatgga gtgggaaaga
     2641 gaaattgaca attatacaga cataatatac aacttacttg aaaaatcgca aaaccagcag
     2701 gaaaagaatg aacaggaatt attagaattg gataagtggg caactttgtg gagttggttt
     2761 gacataacaa actggctgtg gtatataagg atatttataa taatagtagg aggcttaata
     2821 ggtttaagaa tagtttttac tgtattttct atagtgaata gagttaggca gggatactca
     2881 ccattatcgt ttcagacccg attcccagcc ccgaggggac cagacaggcc cgaaggaaca
     2941 gaagaaggag gtggagagag agacagagac acattcaagc cgttagcaac tggattctta
     3001 gcgattttct gggtcgacct gcggaacctg tgcctcttca gctaccaccg cttgatagac
     3061 ttactcttga ttgtaacgag gattgtggaa cttctgggac gcagggggtg ggaaatcctc
     3121 aagtattggt ggagtctcct acaatattgg ggtcaggaac taaagaatag tgctgttagc
     3181 ttgttcaacg ccactgccat aacagtagct gagggaacag ataggattat agaagcagta
     3241 caaagatttg gtagagctgt cctccacata cctagaagaa taagacaggg cttagaaagg
     3301 gctttgcaat aaaatgggtg gcaagtggtc aaaacctagt ttaggtagtg ggtgggctac
     3361 tataagggaa aaaatgagac gagcagagcc agcagcagag ccagcagcag atggggtggg
     3421 agcagtatct cgagacggat ccagctaagg gtcaagacaa ttctgcagat atccagcaca
     3481 gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
     3541 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc
     3601 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     3661 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     3721 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     3781 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     3841 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     3901 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3961 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4021 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4081 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4141 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4201 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     4261 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     4321 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4381 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4441 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4501 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4561 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     4621 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     4681 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     4741 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     4801 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     4861 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     4921 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     4981 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5041 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5101 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     5161 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     5221 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     5281 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     5341 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     5401 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     5461 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     5521 gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     5581 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     5641 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     5701 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5761 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5821 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     5881 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     5941 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6001 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6061 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6121 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6181 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6241 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6301 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6361 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6421 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6481 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6541 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6601 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6661 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6721 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6781 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6841 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6901 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6961 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7021 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7081 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7141 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7201 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7261 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7321 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7381 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7441 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7501 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7561 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7621 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7681 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7741 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7801 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7861 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     7921 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     7981 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     8041 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     8101 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     8161 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     8221 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
     8281 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     8341 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     8401 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     8461 gtatttacgg taaactgccc
//
