LOCUS       sequence-291419-        9935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 9935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: pLentiEKAR2G2
FEATURES             Location/Qualifiers
     source          1..9935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             608..625
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             635..664
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     2059..2080
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             join(2138..2140,2141..2143,2144..2656)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(2183..2204)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2444..2463)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             2789..2806
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    2848..3436
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2901..2921)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3319..3330)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3527..3760
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3832..3953
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3869..3888)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3923..3942
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3972..4107
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4127..4146)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4128..4146)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4156..4173)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4156..4172)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4165..4187)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4314..4769
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4401..4420)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4611..4632
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4795..4899
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4900..4968,4969..5760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5118..5137)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5931..6519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6420..6439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6673..6690
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6807..6828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6843..6848,6849..6866,6867..6873)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6881..6897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6886..6908
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6905..6921
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6905..6921
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6940..6960
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6942..6960
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6988..7214
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7215..7395
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7442..7567
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8060..8293
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8478..8522
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8789..8905
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(8917..8937)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8922..9251
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9102..9237
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9261..9860
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9261..9280)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9757..9777
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagaggatcc ggagatatac
      601 catggcacac catcaccacc accacgggag cggggagcag aaactaatat cagaggagga
      661 tcttggctca ggttcaggat ccgaggacaa gggcatcgtg aaggtgaagt ccgacatctc
      721 catggaggag gactccttca tctacgagat acacctcaag ggcgagaact tcccccccaa
      781 cggccccgtg atgcagaaga agaccaccgg ctgggacgcc tccaccgaga ggatgtacgt
      841 gcgcgacggc gtgctgaagg gcgacgtcaa gcacaagctg ctgctggagg gcggcggcca
      901 ccaccgcgtt gacttcaaga ccatctacag ggccaagaag gcggtgaagc tgcccgacta
      961 tcactttgtg gaccaccgca tcgagatcct gaaccacgac aaggactaca acaaggtgac
     1021 cgtttacgag agcgccgtgg cccgcaactc caccgacggc atggacgagc tgtacaaggg
     1081 tgctagcgga ggaatggtga gcaagggcga ggagaccaca atgggcgtaa tcaagcccga
     1141 catgaagatc aagctgaaga tggagggcaa cgtgaatggc cacgccttcg tgatcgaggg
     1201 cgagggcgag ggcaagccct acgacggcac caacaccatc aacctggagg tgaaggaggg
     1261 agcccccctg cccttctcct acgacattct gaccaccgcg ttcgcctacg gcaacagggc
     1321 cttcaccaag taccccgacg acatccccaa ctacttcaag cagtccttcc ccgagggcta
     1381 ctcttgggag cgcaccatga ccttctccgg aatggcggac gaggagaagc tgccgcccgg
     1441 ctgggagaag cgcatgagcc gcagctcagg ccgagtgtac tacttcaacc acatcactaa
     1501 cgccagccag tgggagcggc ccagcggcaa cagcagcagt ggtggcaaaa acgggcaggg
     1561 ggagcctgcc aggaagctgc gcattctgca gtcgactagg gagcaagggc ttgtgcccgg
     1621 gtctagaggc ggaggtggtg gaggaggtgg aggtggtgga ggtggaggtg gaggtggtgg
     1681 cggtggtgga ggaggaggag ctagaggcgg aggtggtgga ggaggtggag gtggtggagg
     1741 tggaggtgga ggtggtggcg gtggtggagg aggaggagcc agcggcggag gtggtggagg
     1801 aggtggaggt ggtggaggtg gaggtggagg tggtggcggt ggtggaggag gaggaaccgg
     1861 tgctgaccaa ctgactgaag agtggtcgac ggtacagcgg accgcgcctg acgtaccacg
     1921 tacaccagta ggaaaggcaa agctttcatt ccaattcccg cgggcgcggg acatcgcggc
     1981 cgccatgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
     2041 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     2101 cggcatggac gagctgtaca agggtggcag cggtggcatg gtgagcaagg gcgaggagct
     2161 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     2221 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat
     2281 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg
     2341 cctgatgtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     2401 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     2461 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     2521 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     2581 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
     2641 ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
     2701 catcggcgac ggccccgtgg gtaccgatat ccttcaaaaa aaacttgaag aacttgaact
     2761 tgacgaatga gatatctaat gactcgagca ccaccatcac catcaccagg atcctgcagt
     2821 ctagagctag catatgaatt cgtcgacaat caacctctgg attacaaaat ttgtgaaaga
     2881 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     2941 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3001 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     3061 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     3121 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     3181 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     3241 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3301 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3361 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3421 tgggccgcct ccccgcctgg aattaattcg agctcggtac ctttaagacc aatgacttac
     3481 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     3541 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     3601 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     3661 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     3721 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     3781 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     3841 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3901 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     3961 tggctctagc tatcccgccc ctaactccgc ccaktyccgc ccwtwctccg ccccatggct
     4021 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4081 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     4141 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4201 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4261 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     4321 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4381 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4441 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4501 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4561 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4621 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4681 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4741 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     4801 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4861 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4921 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4981 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5041 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5101 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5161 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5221 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5281 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5341 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5401 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5461 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5521 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5581 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5641 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5701 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5761 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5821 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5881 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5941 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6001 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6061 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6121 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6181 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6241 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6301 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6361 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6421 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6481 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6541 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6601 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6661 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6721 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6781 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     6841 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     6901 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     6961 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     7021 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     7081 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     7141 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     7201 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7261 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7321 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7381 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     7441 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     7501 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     7561 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     7621 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     7681 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     7741 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     7801 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     7861 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     7921 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     7981 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     8041 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     8101 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     8161 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     8221 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     8281 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     8341 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     8401 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     8461 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     8521 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     8581 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     8641 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     8701 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     8761 tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca
     8821 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     8881 aacaaattac aaaattcaaa attttatcga ccctgtggaa tgtgtgtcag ttagggtgtg
     8941 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     9001 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     9061 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     9121 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     9181 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     9241 gcttttgcaa aaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     9301 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     9361 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     9421 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     9481 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     9541 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     9601 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     9661 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     9721 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     9781 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     9841 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
     9901 cacgacccca tgcatcgata agcttgggag ttccg
//
