LOCUS       sequence-291422-       10484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-GLO^SEC^3xFlag vector with ccdB markers for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10484)
  TITLE     Unpublished SapTrap/SEC plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDD289
FEATURES             Location/Qualifiers
     source          1..10484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             289..594
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             1539..1559
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(1577..1610)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1620..1649
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3466..3789)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5707..5731
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5734..6759
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5734..6759
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6760..6784)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7137..7170)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7189..7212
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7216..7239
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7243..7266
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(7314..7619)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(7885..7902)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7885..7901)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7894..7916)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8110..8129)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8229..8251
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8289..8307)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8375..8479
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8480..8548,8549..9340)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8698..8717)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9511..10099
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10000..10019
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10253..10270
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10387..10408
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10423..10428,10429..10446,10447..10453)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10461..10477
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(10466..10484,1..4)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 caggaaacag ctatgaccat gttatcgatt cctaggaacc cgaagtatgt caaaaagagg
       61 tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag
      121 gcatatatga tgtcaatatc tccggttcgg taagcacaac catgcagaat gaagcccgtc
      181 gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt
      241 ttattgaaat gaacggctct tttgccgacg agaacagggg ctggtgaaat gcagtttaag
      301 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt
      361 attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat
      421 aaagtccccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg
      481 accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc
      541 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca
      601 ggctccctta tacacagcca gtctgcaggt cgacctaggg agcatcggga gcctcaggag
      661 catcgatgcg ttcttccaaa ggagaagaac tctttaccgg agtcgtccca attctcgtcg
      721 agctcgacgg agacgtaaac ggacacaaat tctcggtttc cggagaagga gaaggagatg
      781 ctacttatgg aaaactcacc cttaaattca tttgcaccac cggaaagctc ccagtgccgt
      841 gggtaagttt aaacatatat atactaacta accctgatta tttaaatttt cagccgaccc
      901 ttgtgactac attctgctac ggagttcaat gcttttcccg ctacccagac cacatgaagc
      961 gtcacgattt cttcaaatca gccatgccag aaggatacgt ccaggagcga acaattttct
     1021 tcaaggacga tggaaactac aagactcgta agtttaaaca gttcggtact aactaaccat
     1081 acatatttaa attttcaggt gctgaagtca agtttgaagg agatactctc gtgaaccgca
     1141 ttgagctcaa gggaatcgac ttcaaagaag atggaaacat tcttggacac aagctcgaat
     1201 acaactataa ctcgcacaac gtgtatatca tggccgacaa gcaaaaaaat ggaatcaagg
     1261 tcgtaagttt aaacatgatt ttactaacta actaatctga tttaaatttt cagaacttca
     1321 agattcgcca caacatcgaa gacgggtcgg ttcaactcgc tgatcactac cagcagaaca
     1381 caccaattgg agatggacca gtcctcctcc ctgataatca ctacctttcc actcaatccg
     1441 ctcttagcaa ggatccaaat gagaaaagag atcacatggt tcttctcgag tttgtcaccg
     1501 ccgccggaat cacccacgga atggacgagt tgtacaaaga gaatctgtac tttcaatccg
     1561 gaaaggtaag tttaaaataa cttcgtatag catacattat acgaagttat tttcagggag
     1621 agcaaaagct tatctccgag gaggacctct aaggatgatc gacgccaacg tcgttgaatt
     1681 ttcaaatttt aaatactgaa tatttgtttt ttttcctatt atttatttat tctctttgtg
     1741 ttttttttct tgctttctaa aaaattaatt caatccaaat ctaaacattt ttttttctct
     1801 ttccgtctcc caattcgtat tccgctcctc tcatctgaac acaatgtgca agtttattta
     1861 tcttctcgct ttcatttcat taggacgtgg ggggaattgg tggaaggggg aaacacacaa
     1921 aaggatgatg gaaatgaaat aaggacacac aatatgcaac aacattcaat tcagaaatat
     1981 ggaggaaggt ttaaaagaaa acataaaaat atatagagga ggaaggaaac tagctgccct
     2041 cgattgtcta tcttccattc tcaccaagtt tcaacaactt tattcagtca ttcttctctg
     2101 gttcatttcc caaccaatct tcccgaataa agcccatctg tcctttcggc cacattcctc
     2161 acctacatcc gtactcctta tctcccgttc ttatccagtc ttttatcgtc tctgcgtctc
     2221 gcacactgtc tcgtcggtaa tgtaaaaaaa aaacaaaaaa aacttgaaaa cttgcggaaa
     2281 gatttcctaa taaaaggaga cgaatccggt aggaaatcga caagtctctg cttctcgaag
     2341 actaggtgag ttacatttct ttggccgaat tattttgaaa tcctaaattt gataatttca
     2401 gagatgtctg taaaacttgc gtgttatgtg acggcttcgg tcactgtcgc cactcttatg
     2461 gtatgcttca tgaccatgtc aaccatctac tcggaggttg atggattcag agagaagctt
     2521 gataccgaga tgaatgtgtt cagagtgagt taaattatat tttgaatttt aaataatttt
     2581 taattttcta gcaatctacc aatggattgt ggaaggacat agttgtcatc ggaagatcta
     2641 gcaagcgtgt ccgttgtcaa tatgaagaga ccaacgctac cccaactcca catgctgatg
     2701 gatccccatc tgctccacca ggtcaaccac cagcagttcc accagtcttc aaccagccaa
     2761 agactccaaa tggagccaat ggaaatggac caacctgcaa ctgcaatgct gataacaagt
     2821 gcccagctgg accatccgga ccaaagggag ttccaggagt tccaggactc gacggagttc
     2881 caggacttga cggtgttcca ggagttggag ctgatgatat cgctccacaa cgcgagtctg
     2941 tcggatgctt cacttgccca caaggaccag ttggaccacc aggagctctt ggaagaccag
     3001 gaccacgtgg acttccagga ccaagaggac aaaatggaaa cccaggaaga gatggacaac
     3061 caggacatcc aggagagcaa ggatcatccg gtcaaatcgg aaagatcgga gagccaggac
     3121 caccaggaga gaagggacgc gacgccgagc atccaatcgg aagaccagga ccaaagggac
     3181 caagaggaga tcaaggacca acaggaccag ctggacagaa cggtcttcac ggaccaccag
     3241 gagagccagg aaccgttgga ccagaaggac catctggaaa gcaaggacgt caaggaccag
     3301 acggaaccca gggagagact ggaccagacg gaagaccagg aaaggatgcc gagtactgcc
     3361 agtgcccaga caagtctcca ccatcagagg ctgtcaacgc caaccgtgga tacagaaata
     3421 tctaaattgt tggtgttttc taataaaata tttgagattc agtgagactt ttttcttggc
     3481 ggcacaataa agtttcctct ttttgttggg tcactcaacg aacacaatac aaatatttga
     3541 gcgctcgcat ttttctcaac ctttcgggtt taaagcactt caaaatggaa ttgaagagag
     3601 attgtgaaag tacatcagac agaatcacat tttgatagca ttcacttcac tcagatgcaa
     3661 acttttcagt gactataaaa agtcataaaa taataacatt aaaaaagcaa ataaattagc
     3721 gagagaattt tttgacaaaa agaaagaaga gtgatagaga agaagggaat gcttgaaagg
     3781 attttgcatt tatcttaatc accgtcttcg agaagacgaa ccatggcgcc agtttcagaa
     3841 tccaagtttc taatatagtt catcacaatg ttgacgttgg tccaacctcc ggcttgcata
     3901 atctctggga tggaaactcc agcgcgagcc atgtcacggg cagctccgac acgggcggaa
     3961 tgtccagacc aagccaagta acgttgtccg gaatcatcct tggctccgta gatgagacgg
     4021 tgggtagctt cgaagattcc tgaaaattta ttttcagctt attcttagct aatatgttgt
     4081 ttaaacttac cttcaagagc tcgagtcgag agttgtgatg ttgcactcgg ggcagcaact
     4141 ccgttcttgc gaacacgaca gaacaggtag ttgtttggat catcggcgac tccggaaacc
     4201 gaaatccagc gctcaacgag cttggtaact ccaagggaga gcgctttttc gactccggca
     4261 gtcgagacca gcgtttttgt acgtccgatg tggatgagca ttcttcctcc gtctgtgcga
     4321 ctaatatcct tgacgcgaat gcgggcaatt tcggcgatac ggagaagtgt gttgtaagca
     4381 attccgagga aggcgaggtt tcggatgtcc tggcaacgat cggaattttc cataagagaa
     4441 cggacctgat cgaagtcggt tctgaaaatt tcgtcaagtc ttaggattga atacattaat
     4501 atttaaactt acctttcgaa ggcgagggct tgcttggctc tctctccagc atcaacattt
     4561 tccttgcgga tacgtctcat tacgagagat acggcattag aatctgatgg gcgtggaagt
     4621 ccagatcggc gatggagcat attgagctgt ccgagatgtt gttggatcgt cttcaccgcc
     4681 aatccacgag cttggaggta gagcaagtag tcacgaacgt cttctggctc ggctgggaac
     4741 cacttgcggt tgttaagctt gcaccaggca gcccaagaac ggcacactga gagaagcatc
     4801 ttccaggtgt gctcggagaa ggcttgtctg tcacggaaca tatccatgag gttcttgcga
     4861 acttcatcac ttgtcgcgtc aactggaagt gctggaagat tttgatgaac agtgagaaga
     4921 tttgacatat tttcgaagtt ttttagatgc actagaacaa agcgtgttgg cttcctctga
     4981 gcccgctttc cttatatacc cgcattctgc agccttacag aatgttctag aaggtcctag
     5041 atgcattcgt ttgaaaatac tcccggtggg tgcaaagaga cgcagacgga aaatgtatct
     5101 gggtctcttt attgtgtaca ctacttttcc atgtaccgaa tgtgagtcgc cctccttttg
     5161 caacaagcag ctcgaatgtt ctagaaaaag gtggaaaata gtataaatac cgttgaaaat
     5221 aaataccgaa caacatttgc tctaattgtg aaattagaaa tcttcaaact ataatcatgt
     5281 cactttacca ctatttccgt ccagctcaac gttccgtttt tggtgatcat ttttgctttc
     5341 gtcgtaaatc tacacacgcg tctcttccgt gcgagagtcc aagccagcag ccaaattcgt
     5401 tgactgagta ttcaacgttt atacgttgtc ggcaacgaga aataggaaaa tgcatcggga
     5461 aatgttcttt tttcgatttt ttccaaggtt ttgacaaatt ttaccacgaa ttttgctatg
     5521 ttttcaatta aaaaatatgt tattcaactg tttctatgag gaaaataagg ctttgcatgt
     5581 aattttctta ttcagcataa tttttaatta atttgaattt tctgtcctaa cgtttatttt
     5641 gttttcttgg ttatgactga tctgaaatta atttttgaat tttaaggtaa tatgtcaggc
     5701 ggtgccgcaa gtttgtacaa aaaagcaggc tccatgaaaa agcctgaact caccgcgacg
     5761 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     5821 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     5881 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     5941 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
     6001 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     6061 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
     6121 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     6181 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     6241 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     6301 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     6361 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     6421 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     6481 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     6541 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     6601 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     6661 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     6721 aaccgacgcc ccagcactcg tccgagggca aaggaataga cccagctttc ttgtacaaag
     6781 tgggtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta
     6841 tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca
     6901 cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa
     6961 aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc
     7021 tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa
     7081 ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa tttttaataa
     7141 cttcgtatag catacattat acgaagttat tttcagggag ccggatctga ttataaagac
     7201 gatgacgata agcgtgacta caaggacgac gacgacaagc gtgattacaa ggatgacgat
     7261 gacaagagac tagttcgacc tgcagactgg ctgtgtataa gggagcctga catttatatt
     7321 ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc
     7381 acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag
     7441 ctttcatccc cgatatgcac caccgggtaa agttcacggg ggactttatc tgacagcaga
     7501 cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt
     7561 acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt aaactgcatt
     7621 tcaccagccc ctgttctcgt cggcaaaaga gccgttcatt tcaataaacc gggcgacctc
     7681 agccatccct tcctgatttt ccgctttcca gcgttcggca cgcagacgac gggcttcatt
     7741 ctgcatggtt gtgcttaccg aaccggagat attgacatca tatatgcctt gagcaactga
     7801 tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcatacct ctttttgaca
     7861 tacttcgggt aactagtgcc ggcgactggc cgtcgtttta caacgtcgtg actgggaaaa
     7921 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     7981 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     8041 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     8101 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     8161 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     8221 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     8281 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     8341 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     8401 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     8461 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     8521 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8581 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     8641 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     8701 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     8761 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8821 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8881 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8941 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     9001 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     9061 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     9121 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     9181 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     9241 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     9301 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     9361 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9421 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9481 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9541 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9601 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     9661 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9721 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9781 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9841 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9901 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9961 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    10021 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    10081 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    10141 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    10201 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    10261 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
    10321 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
    10381 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
    10441 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt caca
//
