LOCUS       sequence-29144-e         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Chen LF, Mu Y, Greene WC
  TITLE     Acetylation of RelA at discrete sites regulates distinct nuclear 
            functions of NF-kappaB.
  JOURNAL   EMBO J. 2002 Dec 2. 21(23):6539-48.
  PUBMED    12456660
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2002 Dec 2. 21(23):6539-48."
COMMENT     Sequence Label: T7-RelA(KR)
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        298..501
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     452..472
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     498..522
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           591..630
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             666..698
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
       61 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      121 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      181 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      241 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      301 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
      361 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      421 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      481 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
      541 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgggg gatcttggtg
      601 gcgtgaaact cccgcacctc ttcggccagc gccttgtaga agcgcgtatg gcttcgtggg
      661 gatctatggc tagcatgact ggtggacagc aaatgggtcg cggatccga
//
