LOCUS       sequence-29151-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  TITLE     Rimm Plasmids
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGEX-Bcatfl
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             98..133
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 catctcaatc ggactgtctg gtggtggtgg tggtctggtt ccgcgtggat ccatggctac
       61 tcaagctgat ttgatggagt tggacatggc catggaacca gacagaaaag cggctgttag
      121 tcactggcag caacagtctt acctggactc tggaatccat tctggtgcca ctaccacagc
      181 tccttctctg agtggtaaag gcaatcctga ggaagaggat gtggatacct cccaagtcct
      241 gtatgagtgg gaacagggat tttctcagtc cttcactcaa gaacaagtag ctgatattga
      301 tggacagtat gcaatgactc gagctcagag ggtacgagct gctatgttcc ctgagacatt
      361 agatgagggc atgcagatcc catctacaca gtttgatgct gctcatccca ctaatgtcca
      421 gcgtttggct gaaccatcac agatgctgaa acatgcagtt gtaaacttga ttaactatca
      481 agatgatgca gaacttgcca cacgtgcaat ccctgaactg acaaaactgc taaatgacga
      541 ggaccaggtg gtggttaata aggctgcagt tatggtccat cagctttcta aaaaggaagc
      601 ttccagacac gctatcatgc gttctcctca gatggtgtct gctattgtac gtaccatgca
      661 gaatacaaat gatgtagaaa cagctcgttg taccgctggg accttgcata acctttccca
      721 tcatcgtgag ggcttactgg ccatctttaa gtctggaggc attcctgccn tggtgaaatg
      781 cttggttcac cagtggattc tgngttg
//
