LOCUS       sequence-29152-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Derijard B, Raingeaud J, Barrett T, Wu IH, Han J, Ulevitch RJ, Davis
            RJ
  TITLE     Independent human MAP-kinase signal transduction pathways defined by
            MEK and MKK isoforms.
  JOURNAL   Science. 1995 Feb 3. 267(5198):682-5.
  PUBMED    7839144
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1995 Feb 3. 267(5198):682-5."
COMMENT     Sequence Label: pCDNA3 Flag MKK4beta
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..119
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tcggcagcca acatggacta taaggacgat gatgacaaaa
      121 tggcggctcc gagcccgagc ggcggcggcg gctccggggg cggcagcggc agcggcaccc
      181 ccggccccgt agggtccccg gcgccaggcc acccggccgt cagcagcatg cagggtaaac
      241 gcaaagcact gaagttgaat tttgcaaatc cacctttcaa atctacagca aggtttactc
      301 tgaatcccaa tcctacagga gttcaaaacc cacacataga gagactgaga acacacagca
      361 ttgagtcatc aggaaaactg aagatctccc ctgaacaaca ctgggatttc actgcagagg
      421 acttgaaaga ccttggagaa attggacgag gagcttatgg ttctgtcaac aaaatggtcc
      481 acaaaccaag tgggcaaata atggcagtta aaagaattcg gtcaacagtg gatgaaaaag
      541 aacaaaaaca acttcttatg gatttggatg tagtaatgcg gagtagtgat tgcccataca
      601 ttgttcagtt ttatggtgca ctcttcagag agggtgactg ttggatctgt atggaactca
      661 tgtctacctc gtttgataag ttttacaaat atgtatatag tgtattagat gatgttattc
      721 cagaagaaat tttaggcaaa atcactttag caactgtgaa agcactaaac cacttaaaag
      781 aaaacttgaa a
//
