LOCUS       sequence-29155-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Hubner A, Barrett T, Flavell RA, Davis RJ
  TITLE     Multisite phosphorylation regulates Bim stability and apoptotic 
            activity.
  JOURNAL   Mol Cell. 2008 May 23. 30(4):415-25.
  PUBMED    18498746
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 May 23. 30(4):415-25."
COMMENT     Sequence Label: pCMV-Tag2B Flag BimEL(TD)
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        50..68
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             112..135
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(762..778)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(763..779)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gagctggttt agtgancgtc agatccgcta gcgattacgc caagctcgaa attaaccctc
       61 actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgccaccat ggattacaag
      121 gatgacgacg ataagagccc gggcggatca atggccaagc aaccttctga tgtaagttct
      181 gagtgtgaca gagaaggtgg acaattgcag cctgctgaga ggcctcccca gctcaggcct
      241 ggggccccta cctccctaca gacagaaccg caaggtaatc ccgacggcga aggggaccgc
      301 tgcccccacg gcagccctca gggcccgctg gccccaccgg ccagccctgg cccttttgct
      361 accagatccc cacttttcat ctttgtgaga agatcttctc tgctgtcccg gtcctccagt
      421 gggtatttct cttttgacac agacaggagc ccggcaccca tgagttgtga caagtcaaca
      481 caggatccaa gtcctccttg ccaggccttc aaccactatc tcagtgcaat ggcttccata
      541 agacagtctc aagaggaacc tgaagatctg cgcccggaga tacggattgc acaggagcta
      601 cggcggatcg gagacgagtt caacgaaact tacacaagga gggtgtttgc aaatgattac
      661 cgcgaggctg aagaccaccc tcaaatggtt atcttacaac tgttacgctt tatcttccgt
      721 ctggtatgga gaanncattg agaattcgat atcaagctta tcgataccgt cgacctcgag
      781 ggggggcccg gtaccttaaa t
//
