LOCUS       sequence-29161-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Hubner A, Barrett T, Flavell RA, Davis RJ
  TITLE     Multisite phosphorylation regulates Bim stability and apoptotic 
            activity.
  JOURNAL   Mol Cell. 2008 May 23. 30(4):415-25.
  PUBMED    18498746
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 May 23. 30(4):415-25."
COMMENT     Sequence Label: pCMV-Tag2B Flag BimS
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        50..68
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             112..135
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(504..520)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(505..521)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(576..595)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(577..595)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctggttt agtgancgtc agatccgcta gcgattacgc caagctcgaa attaaccctc
       61 actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgccaccat ggattacaag
      121 gatgacgacg ataagagccc gggcggatcc atggccaagc aaccttctga tgtaagttct
      181 gagtgtgaca gagaaggtgg acaattgcag cctgctgaga ggcctcccca gctcaggcct
      241 ggggccccta cctccctaca gacagaaccg caagcttcca taagacagtc tcaagaggaa
      301 cctgaagatc tgcgcccgga gatacggatt gcacaggagc tacggcggat cggagacgag
      361 ttcaacgaaa cttacacaag gagggtgttt gcaaatgatt accgcgaggc tgaagaccac
      421 cctcaaatgg ttatcttaca actgttacgc tttatcttcc gtctggtatg gagaaggcat
      481 tgagaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc cggtacctta
      541 attaattaag gtaccaggta agtgtaccca attcgcccta tagtgagtcg tattacaatt
      601 cactcgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaga tccaattttt
      661 aagtgtatnn tgtgttaaac tactgattct aattgtttgt gtattttaga ttcacagtcc
      721 ca
//
